##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630729.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1028499
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.01763832536541	33.0	31.0	34.0	30.0	34.0
2	32.176486316467006	33.0	31.0	34.0	30.0	34.0
3	32.26201872826323	34.0	31.0	34.0	30.0	34.0
4	35.85591429840962	37.0	35.0	37.0	35.0	37.0
5	35.825467015524566	37.0	35.0	37.0	35.0	37.0
6	35.91682442083074	37.0	35.0	37.0	35.0	37.0
7	35.861249257412986	37.0	35.0	37.0	35.0	37.0
8	35.87993862901179	37.0	35.0	37.0	35.0	37.0
9	37.55207345850604	39.0	37.0	39.0	35.0	39.0
10	37.47826881698475	39.0	37.0	39.0	35.0	39.0
11	37.578862983824	39.0	37.0	39.0	35.0	39.0
12	37.53642055072489	39.0	37.0	39.0	35.0	39.0
13	37.57847503983961	39.0	37.0	39.0	35.0	39.0
14	38.830198182010875	40.0	38.0	41.0	35.0	41.0
15	38.83088073007363	40.0	38.0	41.0	35.0	41.0
16	38.78247134902416	40.0	38.0	41.0	35.0	41.0
17	38.81651124600024	40.0	38.0	41.0	35.0	41.0
18	38.830256519452135	40.0	38.0	41.0	35.0	41.0
19	38.89758667728408	40.0	38.0	41.0	35.0	41.0
20	38.88108690431395	40.0	38.0	41.0	35.0	41.0
21	38.834004700053185	40.0	38.0	41.0	35.0	41.0
22	38.74833713985137	40.0	38.0	41.0	35.0	41.0
23	38.68268807261845	40.0	38.0	41.0	34.0	41.0
24	38.62017269827195	40.0	38.0	41.0	34.0	41.0
25	38.56956788484967	40.0	38.0	41.0	34.0	41.0
26	38.39871404833646	40.0	38.0	41.0	34.0	41.0
27	38.23823260887954	40.0	38.0	41.0	34.0	41.0
28	38.131951513807984	40.0	37.0	41.0	33.0	41.0
29	38.031630560651976	40.0	37.0	41.0	33.0	41.0
30	37.90243062949016	40.0	37.0	41.0	33.0	41.0
31	37.791234604992326	40.0	37.0	41.0	33.0	41.0
32	37.65147268009011	40.0	36.0	41.0	33.0	41.0
33	37.5207238898628	40.0	36.0	41.0	33.0	41.0
34	37.432337804898204	40.0	36.0	41.0	33.0	41.0
35	37.32332846215699	40.0	36.0	41.0	33.0	41.0
36	37.226932646507194	40.0	36.0	41.0	32.0	41.0
37	37.03101510064667	40.0	35.0	41.0	31.0	41.0
38	36.82830415975125	39.0	35.0	41.0	31.0	41.0
39	36.69452279486903	39.0	35.0	41.0	31.0	41.0
40	36.43443698049293	39.0	35.0	41.0	30.0	41.0
41	36.296279335225414	39.0	35.0	41.0	30.0	41.0
42	36.08215856310993	39.0	35.0	41.0	29.0	41.0
43	35.31668868905074	38.0	35.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	9.0
11	7.0
12	3.0
13	5.0
14	7.0
15	2.0
16	5.0
17	12.0
18	37.0
19	67.0
20	147.0
21	291.0
22	581.0
23	957.0
24	1597.0
25	2686.0
26	4218.0
27	6381.0
28	9092.0
29	12502.0
30	16439.0
31	20960.0
32	26612.0
33	34420.0
34	44331.0
35	58695.0
36	81741.0
37	132480.0
38	222909.0
39	351305.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.984000956734036	19.710276820881692	12.832875870564775	22.472846351819495
2	18.396128727397887	22.302987168679795	33.997796789301695	25.303087314620626
3	21.211688100814875	24.69025249416869	29.824919615867394	24.27313978914904
4	14.078088554291254	15.70375858411141	34.3063046245062	35.911848237091135
5	13.604680218454272	38.83319283732896	33.34626479947963	14.215862144737137
6	33.706692957406865	34.20226952092321	16.28956372344553	15.801473798224402
7	27.63959906621202	32.182238388175385	21.81023024815775	18.367932297454832
8	28.75831673146984	31.86595222746935	20.487331538484725	18.888399502576085
9	26.92992409326601	13.424223066818733	21.49783325020248	38.14801958971278
10	17.918442312535063	28.13643960762237	32.17407114639878	21.771046933443785
11	33.31029004403504	23.034246994892555	21.485582387537566	22.16988057353483
12	21.609160533943154	27.931675188794546	29.115828017334	21.343336259928304
13	30.176208241330325	20.428216264673082	26.522339836985743	22.87323565701085
14	22.576589768196175	22.124766285625945	27.68325491808937	27.615389028088504
15	25.450778270080963	28.06371226418305	24.677904402435004	21.807605063300986
16	23.146352111183386	26.796136894639666	26.88228184956913	23.175229144607822
17	21.819953155034668	27.609944200237436	27.723896668834875	22.846205975893024
18	21.248246230672077	25.249708555866363	30.250977395213802	23.251067818247755
19	22.323405273121317	26.093754101851342	30.726816457770013	20.856024167257335
20	23.300654643320023	25.129922343142773	30.096869321214704	21.472553692322503
21	23.769590441993625	25.3841763579741	29.413057280561283	21.433175919470994
22	23.300849101457562	25.837847192850944	29.629100271366333	21.232203434325168
23	22.564338905531265	25.847667328796625	29.234350252163587	22.35364351350852
24	21.82180050734128	26.779996869223986	29.75297010497823	21.64523251845651
25	22.10716782417873	26.7153395384925	29.287145636505237	21.890347000823528
26	21.907264858789365	26.989039367077655	29.400125814414984	21.703569959717996
27	21.944406363059176	26.41840196247152	29.54528881408732	22.091902860381975
28	21.45135775533083	26.784761093593673	29.896966355825334	21.866914795250167
29	21.32894635775047	27.398859891939615	30.068186745927804	21.204007004382113
30	21.654760967195884	27.547036992743795	29.42171066768174	21.376491372378585
31	21.080234399838986	27.99030431726234	29.50348031451659	21.42598096838208
32	20.67508087027795	27.47362904582309	29.58904189503344	22.26224818886552
33	20.295401356734423	27.669837306599227	30.0849101457561	21.94985119091025
34	21.700264171379846	26.82715296757702	29.558025822096084	21.914557038947045
35	21.038134213061948	27.077809506863886	29.72730163082317	22.156754649250995
36	20.617326803429076	27.844071797833543	29.3641510589704	22.17445033976698
37	21.057774484953313	26.902505495873115	29.82550299028001	22.21421702889356
38	20.9853388287203	26.900658143566496	29.764734822299292	22.349268205413907
39	20.905902679535906	26.65146004031117	30.136636010341284	22.306001269811638
40	20.163169823208385	26.607706959365057	30.762110609733213	22.46701260769335
41	19.631326817041145	26.91699262711972	30.798474281452876	22.653206274386264
42	19.935751031357345	26.794775687676896	30.927302797571997	22.34217048339376
43	19.88188612725924	26.566676292344475	30.47382642083269	23.077611159563595
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	517.0
1	452.5
2	388.0
3	973.0
4	1558.0
5	1558.0
6	2252.5
7	2947.0
8	3006.0
9	3065.0
10	4391.5
11	5718.0
12	5718.0
13	9896.0
14	14074.0
15	19493.0
16	24912.0
17	23064.0
18	21216.0
19	21216.0
20	23324.5
21	25433.0
22	21540.5
23	17648.0
24	19017.5
25	20387.0
26	20387.0
27	22522.5
28	24658.0
29	26993.5
30	29329.0
31	32156.5
32	34984.0
33	34984.0
34	37906.5
35	40829.0
36	43717.0
37	46605.0
38	49113.0
39	51621.0
40	51621.0
41	53398.5
42	55176.0
43	56072.0
44	56968.0
45	58282.0
46	59596.0
47	59596.0
48	61614.5
49	63633.0
50	61992.0
51	60351.0
52	59755.5
53	59160.0
54	59160.0
55	57646.0
56	56132.0
57	54762.5
58	53393.0
59	52080.0
60	50767.0
61	50767.0
62	46848.0
63	42929.0
64	39107.0
65	35285.0
66	30089.0
67	24893.0
68	24893.0
69	21746.5
70	18600.0
71	15539.0
72	12478.0
73	9376.5
74	6275.0
75	6275.0
76	4557.5
77	2840.0
78	2233.0
79	1626.0
80	1327.0
81	1028.0
82	1028.0
83	808.0
84	588.0
85	501.0
86	414.0
87	353.0
88	292.0
89	292.0
90	228.0
91	164.0
92	90.5
93	17.0
94	10.5
95	4.0
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1028499.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.204544570738285
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.08585016983596	54.793401929409356
2	7.754441187858791	9.647229649826617
3	1.6477862520609035	3.074993800781168
4	0.6458592727163793	1.6070152766444252
5	0.35858135594431584	1.115269496903698
6	0.23047148461095104	0.8601824242059676
7	0.14686815996245287	0.6395106901684703
8	0.1231907833938039	0.6130421261059232
9	0.09949805859952568	0.5570308278770513
>10	0.7242004469968117	8.906259738622012
>50	0.10414281677133884	4.593984144326357
>100	0.07504507022615386	8.590163499798953
>500	0.0021888143968157997	0.8688583192541196
>1k	0.0012507510838947427	0.9226392293221215
>5k	4.690316564605286E-4	2.202877746745586
>10k+	1.5634388548684284E-4	1.0075411000081351
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	10360	1.0072931524483737	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	9960	0.9684015249407145	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7469	0.7262039146367668	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5222	0.5077301971124911	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1427	0.13874588113357425	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1324	0.12873128705035203	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1233	0.11988344179235955	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	1192	0.11589704997282448	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	1154	0.11220234535959685	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1120	0.10889655702144582	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	1036	0.10072931524483739	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.889162750765922E-4	0.0
3	0.0	0.0	0.0	7.778325501531844E-4	0.0
4	0.0	0.0	0.0	8.750616189223324E-4	0.0
5	0.0	0.0	0.0	8.750616189223324E-4	0.0
6	0.0	0.0	0.0	8.750616189223324E-4	0.0
7	0.0	0.0	0.0	8.750616189223324E-4	0.0
8	0.0	0.0	0.0	9.722906876914805E-4	0.0
9	0.0	0.0	0.0	0.0013612069627680727	0.0
10	0.0	0.0	0.0	0.002041810444152109	0.0
11	0.0	0.0	0.0	0.003597475544458478	0.0
12	9.722906876914805E-5	0.0	0.0	0.004569766232149958	0.0
13	9.722906876914805E-5	0.0	0.0	0.005250369713533994	0.0
14	2.9168720630744417E-4	0.0	0.0	0.006611576676302067	0.0
15	2.9168720630744417E-4	0.0	0.0	0.009139532464299917	0.0
16	2.9168720630744417E-4	0.0	0.0	0.016723399828293465	0.0
17	2.9168720630744417E-4	9.722906876914805E-5	0.0	0.025765703223824234	0.0
18	3.889162750765922E-4	9.722906876914805E-5	0.0	0.0297520950433593	0.0
19	3.889162750765922E-4	9.722906876914805E-5	0.0	0.035683068238277334	0.0
20	3.889162750765922E-4	9.722906876914805E-5	0.0	0.04054452167673474	0.0
21	3.889162750765922E-4	9.722906876914805E-5	0.0	0.05153140644764847	0.0
22	3.889162750765922E-4	9.722906876914805E-5	0.0	0.07292180157686104	0.0
23	3.889162750765922E-4	9.722906876914805E-5	0.0	0.10257666755145119	0.0
24	3.889162750765922E-4	9.722906876914805E-5	0.0	0.14807987173541248	0.0
25	3.889162750765922E-4	9.722906876914805E-5	0.0	0.15935844371263366	0.0
26	3.889162750765922E-4	9.722906876914805E-5	0.0	0.17773473771000264	0.0
27	3.889162750765922E-4	9.722906876914805E-5	0.0	0.20573670951551729	0.0
28	3.889162750765922E-4	9.722906876914805E-5	0.0	0.25299003693732325	0.0
29	3.889162750765922E-4	9.722906876914805E-5	0.0	0.33174558264033316	0.0
30	3.889162750765922E-4	9.722906876914805E-5	0.0	0.4600879534156086	0.0
31	3.889162750765922E-4	9.722906876914805E-5	0.0	0.8249886485062212	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTAGA	45	0.0038255383	20.555555	2
AGCGTAT	55	5.1425875E-4	20.181818	14
CGTAGAC	60	9.2359167E-4	18.5	3
AACGCTA	70	1.2190391E-4	18.5	33
TTGCGCG	90	2.151768E-6	18.5	18
GCAGTCG	115	6.4030246E-8	17.695652	9
ATTAGGC	85	2.7226644E-5	17.411764	3
ACGCTAA	75	2.067E-4	17.266666	34
GTATTGG	65	0.0015797814	17.076923	1
TAACGCT	65	0.0015797814	17.076923	32
ATACCGT	110	7.805629E-7	16.818182	6
CAGTCGG	100	5.87658E-6	16.650002	10
TCTATAC	80	3.3821326E-4	16.1875	3
GGTATCA	4795	0.0	15.934307	1
CAATACG	140	3.4713594E-8	15.857142	4
CTCTATG	270	0.0	15.759258	1
AAGACGG	165	9.731593E-10	15.69697	5
TCGGTGA	120	1.9349209E-6	15.416666	13
ATAAGAC	145	5.3427357E-8	15.310345	3
CTTAACG	85	5.363469E-4	15.235294	30
>>END_MODULE