FastQCFastQC Report
Fri 10 Feb 2017
ERR1630728.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630728.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences358146
Sequences flagged as poor quality0
Sequence length43
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT153744.292662768814952No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT146444.088835279467033No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT116273.2464413954085765No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83902.342620048806911No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT26970.7530448476319713No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23080.6444299252260252No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21440.5986385440574514No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA15650.4369726312732796No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG15120.4221741971151429No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT13920.388668308455211No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13390.3738698742970744No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA12070.3370133967711492No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11800.3294745718226645No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG9610.2683263250182886No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9410.2627420102416333No Hit
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT8520.23789180948551708RNA PCR Primer, Index 47 (95% over 22bp)
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA8230.22979455305936683No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA8030.22421023828271153No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGT7790.21750906055072514No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7610.21248317725173532No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT7200.20103533195959192No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA6670.18623689780145528No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC6360.1775812098976395No Hit
GAACAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAA6340.17702277841997396No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA6230.17395140529281353No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA5860.16362042295600118No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA5710.15943218687350968No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA5290.1477051258425335No Hit
GAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAA5120.14295845828237647No Hit
CATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC5010.13988708515521603No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA4990.1393286536775505No Hit
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCC4760.13290669168439687No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG4720.1317898287290658No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA4590.12816002412423982No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA4570.1276015926465743No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC4550.12704316116890876No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA4390.1225757093475845No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4270.1192251204815913No Hit
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATG4230.11810825752626025No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT4190.11699139457092918No Hit
GTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAA4020.11224472701077215No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG3930.10973178536127724No Hit
GTACATGGGAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAA3900.10889413814477895No Hit
GTACATGGGAATGGTATCAACGCAGAAAAAAAAAAAAAAAAAA3780.10554354927878573No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA3700.10330982336812361No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCATTG250.00549135429.611
TGTTCTG250.00549135429.65
ATACACT702.055458E-1029.071434
GATACAC751.3669705E-824.6666663
CCGTCTT1700.022.85294237
GCCGTCT1700.022.85294236
TACACTG502.6970712E-422.25
TGAACCA450.003820742220.5555575
GCAGCGT555.1335973E-420.1818181
TGGAGCT951.668086E-719.4736844
TGCCGTC2100.018.50000235
TTGGAGC801.6116117E-518.53
CGTCTTC701.216225E-418.537
GAGCACA500.00702565818.51
ACACTGT500.00702565818.56
ATGCCGT2150.018.06976734
TATGCCG2200.017.6590933
AGTGGCT752.0622631E-417.26666627
GTGGCTG752.0622631E-417.26666628
TTTGGAG752.0622631E-417.2666662
Produced by FastQC (version 0.11.5)