##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630728.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	358146
Sequences flagged as poor quality	0
Sequence length	43
%GC	32
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.33596075343575	31.0	31.0	34.0	30.0	34.0
2	31.33402578836564	31.0	31.0	34.0	28.0	34.0
3	31.248577395810646	31.0	31.0	34.0	28.0	34.0
4	35.08863145197768	37.0	35.0	37.0	32.0	37.0
5	35.06721560480921	35.0	35.0	37.0	32.0	37.0
6	35.260809837328914	37.0	35.0	37.0	32.0	37.0
7	35.11456221764308	37.0	35.0	37.0	32.0	37.0
8	35.281834782463015	37.0	35.0	37.0	32.0	37.0
9	36.59234502130416	38.0	35.0	39.0	32.0	39.0
10	36.51539316368185	38.0	35.0	39.0	32.0	39.0
11	36.7751643184623	39.0	37.0	39.0	32.0	39.0
12	36.77498282823206	38.0	37.0	39.0	32.0	39.0
13	36.83777844789555	39.0	37.0	39.0	32.0	39.0
14	37.68174152440625	39.0	37.0	41.0	32.0	41.0
15	37.58610454954125	39.0	37.0	41.0	32.0	41.0
16	37.39681023939958	39.0	36.0	40.0	32.0	41.0
17	37.67876508463029	39.0	37.0	40.0	33.0	41.0
18	37.80849709336416	39.0	37.0	40.0	33.0	41.0
19	38.01441032428116	40.0	37.0	41.0	33.0	41.0
20	38.048013938449685	40.0	37.0	41.0	33.0	41.0
21	38.0328916140345	40.0	37.0	41.0	33.0	41.0
22	37.862779425150634	40.0	37.0	41.0	33.0	41.0
23	37.68694052146331	40.0	36.0	41.0	33.0	41.0
24	37.51358943000899	39.0	36.0	41.0	33.0	41.0
25	37.38747326509301	39.0	36.0	41.0	32.0	41.0
26	36.9463207742094	38.0	35.0	40.0	32.0	41.0
27	36.56375891396246	38.0	35.0	40.0	31.0	41.0
28	36.285782893010115	38.0	35.0	40.0	31.0	41.0
29	35.94656927621696	38.0	35.0	40.0	30.0	41.0
30	35.53721387367163	37.0	35.0	40.0	30.0	41.0
31	35.0465983146538	36.0	34.0	40.0	29.0	41.0
32	34.55477375148682	35.0	34.0	39.0	27.0	41.0
33	34.08427010213712	35.0	33.0	39.0	25.0	41.0
34	33.69769311956576	35.0	33.0	39.0	22.0	41.0
35	33.2407900688546	35.0	33.0	39.0	21.0	41.0
36	32.72274156349645	35.0	33.0	39.0	18.0	41.0
37	32.05042915459059	35.0	33.0	38.0	15.0	40.0
38	31.366869935724537	35.0	31.0	38.0	10.0	40.0
39	30.708694219675774	35.0	30.0	38.0	8.0	40.0
40	29.891150536373434	35.0	25.0	38.0	7.0	40.0
41	29.174166401411714	35.0	22.0	38.0	7.0	40.0
42	28.36162626414926	35.0	19.0	37.0	7.0	40.0
43	27.520527941118985	34.0	16.0	36.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	0.0
12	1.0
13	1.0
14	4.0
15	5.0
16	16.0
17	27.0
18	50.0
19	131.0
20	279.0
21	572.0
22	944.0
23	1479.0
24	2346.0
25	3549.0
26	5082.0
27	7132.0
28	9504.0
29	12131.0
30	14178.0
31	16531.0
32	19344.0
33	23071.0
34	26954.0
35	33259.0
36	48068.0
37	66170.0
38	50321.0
39	16993.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	52.99654330915325	20.52235680420834	14.310365046656951	12.17073483998146
2	15.599783328586666	28.910276814483478	32.13940683408442	23.350533022845433
3	26.691628553718317	32.40605786466972	28.796356793039713	12.105956788572259
4	13.903826930916441	18.271877949216243	28.26919747812345	39.55509764174387
5	9.357077839763672	49.07719198315771	27.106263925884978	14.459466251193648
6	28.458784964790894	28.051967633311552	20.44641012324583	23.042837278651724
7	21.14026123424525	39.94627889184857	23.51359501432377	15.399864859582404
8	35.44727569203621	27.73338247530337	21.216766346685432	15.602575485974993
9	29.84481189235675	10.732773784992713	26.364387707806316	33.05802661484422
10	21.15198829527623	34.635037107771694	25.60799227130835	18.604982325643732
11	23.18802946284476	27.981046835648034	20.32578892407007	28.505134777437135
12	19.21730244090399	36.38181076990948	30.790236384044494	13.610650405142035
13	28.929263484724103	21.211461247647605	34.78720968543555	15.072065582192737
14	22.838730573564973	25.08837178134057	37.34454663740486	14.728351007689602
15	26.91723487069519	23.801187225321517	35.40232195808413	13.879255945899157
16	16.57536312006835	25.70711385859398	39.84129377404745	17.876229247290212
17	14.274904647825187	32.002591122056366	37.545023537886784	16.17748069223166
18	12.375120760807047	23.84055664449694	43.88350002512942	19.9008225695666
19	13.756401020812742	28.892686222937016	46.9297437357949	10.421169020455345
20	15.360774656145818	23.848095469445422	48.33671184377321	12.454418030635551
21	18.708292149011854	24.722599163469646	44.67647272341447	11.892635964104024
22	16.778632177938608	26.110022169729664	44.05773064616106	13.053615006170668
23	14.893925940817432	27.661065598945683	43.914492972139854	13.530515488097034
24	12.256174856064286	30.074048013938448	44.82976216403366	12.8400149659636
25	12.275440742043747	29.61892635964104	44.578747214823004	13.526885683492207
26	13.704466893389847	29.303971006237678	44.2685385289798	12.723023571392671
27	11.879792040117717	31.368492179167156	44.12474242348093	12.626973357234201
28	12.007393632764291	31.02310231023102	45.51579523434577	11.453708822658916
29	11.168071121832995	32.4613425809586	45.114283001904255	11.25630329530415
30	10.608522781212132	33.79459773388506	45.291026564585394	10.305852920317413
31	10.624158862586766	34.08414445505464	43.63443958609059	11.657257096268001
32	9.411804124574893	35.43750314117706	44.001329066916846	11.1493636673312
33	9.929749320109677	35.11026229526506	43.84105923282684	11.11892915179843
34	10.060143070144578	36.1570421001491	41.411882304981766	12.370932524724553
35	9.986988546570393	36.434582544548874	41.36218190346953	12.216247005411201
36	10.33181998402886	36.521697855064694	39.80862553260402	13.337856628302426
37	10.353878027396648	36.78918653286649	39.8049957279992	13.05193971173767
38	10.827707136195853	35.99230481423777	39.49478704215599	13.685201007410386
39	11.281991143276764	36.31675350276144	39.34903642648529	13.052218927476503
40	10.949165982588106	36.45664058791666	39.062002646965205	13.53219078253003
41	10.610756507122794	37.384753703796775	37.73656553472606	14.26792425435437
42	10.74980594506151	36.9801700982281	37.6209702188493	14.649053737861095
43	10.478966678393729	37.75415612627253	36.37985625973765	15.387020935596096
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	43.0
1	134.0
2	225.0
3	928.5
4	1632.0
5	1632.0
6	2724.5
7	3817.0
8	4387.5
9	4958.0
10	6899.0
11	8840.0
12	8840.0
13	15661.5
14	22483.0
15	30990.5
16	39498.0
17	35607.5
18	31717.0
19	31717.0
20	32366.0
21	33015.0
22	24022.0
23	15029.0
24	13262.0
25	11495.0
26	11495.0
27	11856.5
28	12218.0
29	12219.5
30	12221.0
31	11823.0
32	11425.0
33	11425.0
34	10627.5
35	9830.0
36	9373.0
37	8916.0
38	9510.0
39	10104.0
40	10104.0
41	11027.5
42	11951.0
43	12546.0
44	13141.0
45	15206.5
46	17272.0
47	17272.0
48	18656.0
49	20040.0
50	17103.5
51	14167.0
52	11596.5
53	9026.0
54	9026.0
55	7405.0
56	5784.0
57	6313.0
58	6842.0
59	6164.0
60	5486.0
61	5486.0
62	4755.5
63	4025.0
64	3745.0
65	3465.0
66	3197.0
67	2929.0
68	2929.0
69	2660.5
70	2392.0
71	2024.0
72	1656.0
73	1410.0
74	1164.0
75	1164.0
76	932.5
77	701.0
78	524.5
79	348.0
80	259.5
81	171.0
82	171.0
83	124.0
84	77.0
85	55.0
86	33.0
87	19.5
88	6.0
89	6.0
90	4.0
91	2.0
92	2.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	358146.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.4899801921875
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.37744194127589	30.508386864693215
2	9.319276592757234	6.987589897359707
3	3.0126949494294375	3.3883762193763887
4	1.563512973033985	2.3446428155708916
5	0.9034475574408612	1.6935115516569035
6	0.5813500763107646	1.3076881707370356
7	0.4667854326390881	1.224984363654876
8	0.32632663724086347	0.9787183333074503
9	0.27180199012596834	0.9170866103417727
>10	1.8174552643122246	13.254672157498753
>50	0.18443345768075625	4.763662283091431
>100	0.1530723029334212	10.997825710513784
>500	0.012693800731064197	3.3309524303052798
>1k	0.006720247445857515	4.295330203429213
>5k	7.46694160650835E-4	2.348658785634196
>10k+	0.0022400824819525053	11.657913602829092
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	15374	4.292662768814952	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	14644	4.088835279467033	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	11627	3.2464413954085765	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8390	2.342620048806911	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2697	0.7530448476319713	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2308	0.6444299252260252	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2144	0.5986385440574514	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	1565	0.4369726312732796	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	1512	0.4221741971151429	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	1392	0.388668308455211	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1339	0.3738698742970744	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	1207	0.3370133967711492	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1180	0.3294745718226645	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	961	0.2683263250182886	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	941	0.2627420102416333	No Hit
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT	852	0.23789180948551708	RNA PCR Primer, Index 47 (95% over 22bp)
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	823	0.22979455305936683	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	803	0.22421023828271153	No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGT	779	0.21750906055072514	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	761	0.21248317725173532	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT	720	0.20103533195959192	No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	667	0.18623689780145528	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	636	0.1775812098976395	No Hit
GAACAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAA	634	0.17702277841997396	No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA	623	0.17395140529281353	No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA	586	0.16362042295600118	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	571	0.15943218687350968	No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA	529	0.1477051258425335	No Hit
GAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAA	512	0.14295845828237647	No Hit
CATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC	501	0.13988708515521603	No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA	499	0.1393286536775505	No Hit
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCC	476	0.13290669168439687	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	472	0.1317898287290658	No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA	459	0.12816002412423982	No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA	457	0.1276015926465743	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC	455	0.12704316116890876	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	439	0.1225757093475845	No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	427	0.1192251204815913	No Hit
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATG	423	0.11810825752626025	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT	419	0.11699139457092918	No Hit
GTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAA	402	0.11224472701077215	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG	393	0.10973178536127724	No Hit
GTACATGGGAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	390	0.10889413814477895	No Hit
GTACATGGGAATGGTATCAACGCAGAAAAAAAAAAAAAAAAAA	378	0.10554354927878573	No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	370	0.10330982336812361	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	8.376472164982996E-4	0.0
3	0.0	0.0	0.0	8.376472164982996E-4	0.0
4	0.0	0.0	0.0	0.001954510171829366	0.0
5	0.0	0.0	0.0	0.0022337259106621323	0.0
6	0.0	0.0	0.0	0.002792157388327665	0.0
7	0.0	0.0	0.0	0.0033505888659931984	0.0
8	0.0	0.0	0.0	0.003629804604825965	0.0
9	0.0	0.0	0.0	0.008934903642648529	0.0
10	0.0	0.0	0.0	0.013402355463972794	0.0
11	0.0	0.0	0.0	0.016194512852300457	0.0
12	0.0	0.0	0.0	0.019824317457126425	0.0
13	0.0	0.0	0.0	0.02233725910662132	0.0
14	0.0	0.0	0.0	0.028480005360942185	0.0
15	0.0	0.0	0.0	0.03629804604825965	0.0
16	0.0	0.0	0.0	0.062265109759706935	0.0
17	0.0	0.0	0.0	0.09660864563613722	0.0
18	0.0	0.0	0.0	0.10945256962244448	0.0
19	0.0	0.0	0.0	0.14295845828237647	0.0
20	0.0	0.0	0.0	0.16222434426183735	0.0
21	0.0	0.0	0.0	0.21471690316239747	0.0
22	0.0	0.0	0.0	0.2979231933345619	0.0
23	0.0	0.0	0.0	0.4098887046065013	0.0
24	0.0	0.0	0.0	0.6070150162224345	0.0
25	0.0	0.0	0.0	0.6483389455696839	0.0
26	0.0	0.0	0.0	0.7231687635768653	0.0
27	0.0	0.0	0.0	0.767843281790108	0.0
28	0.0	0.0	0.0	0.8303876072886477	0.0
29	0.0	0.0	0.0	0.9336974306567712	0.0
30	0.0	0.0	0.0	1.1082072674272503	0.0
31	0.0	0.0	0.0	1.5770104929274653	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCATTG	25	0.005491354	29.6	11
TGTTCTG	25	0.005491354	29.6	5
ATACACT	70	2.055458E-10	29.07143	4
GATACAC	75	1.3669705E-8	24.666666	3
CCGTCTT	170	0.0	22.852942	37
GCCGTCT	170	0.0	22.852942	36
TACACTG	50	2.6970712E-4	22.2	5
TGAACCA	45	0.0038207422	20.555557	5
GCAGCGT	55	5.1335973E-4	20.181818	1
TGGAGCT	95	1.668086E-7	19.473684	4
TGCCGTC	210	0.0	18.500002	35
TTGGAGC	80	1.6116117E-5	18.5	3
CGTCTTC	70	1.216225E-4	18.5	37
GAGCACA	50	0.007025658	18.5	1
ACACTGT	50	0.007025658	18.5	6
ATGCCGT	215	0.0	18.069767	34
TATGCCG	220	0.0	17.65909	33
AGTGGCT	75	2.0622631E-4	17.266666	27
GTGGCTG	75	2.0622631E-4	17.266666	28
TTTGGAG	75	2.0622631E-4	17.266666	2
>>END_MODULE