FastQCFastQC Report
Fri 10 Feb 2017
ERR1630727.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630727.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1800584
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG59270.32917098008201784No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT52780.2931271187570255No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG51030.28340804983272094No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG48550.26963474072856364No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA44680.24814171402167298No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC43750.24297672310761398No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT41050.22798158819582978No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT37770.20976527615484755No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT35320.19615857966082118No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC35050.19465906616964274No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA34640.19238202716451996No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA33890.18821671191124656No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC33850.18799456176440535No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG33430.18566198522257224No Hit
GCCAAGAAGTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAG33240.1846067720250763No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT33060.1836070963642907No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC30790.17100007553104993No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC30680.1703891626272365No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT29860.16583508461699092No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG29650.1646687963460744No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC29640.16461325880936406No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA29640.16461325880936406No Hit
GTTCCTGCTTCCCCGCGGCAGCAGCCAGGGACTTCTGCAGAAA28730.1595593429687257No Hit
GTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCA28660.15917058021175354No Hit
CCCTCGGACCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGG26540.14739662242916743No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC24990.1387883042390691No Hit
GATCAGCAGGCAGCAGCGATGGCTCCGAACCTCGCTCCTAAAG22630.12568144557543554No Hit
GAGTTTGACCAGCCACTCTCCAGCTCGGCTTTCGCGGCGCCGA22500.12495945759820148No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT22440.1246262323779396No Hit
GGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTG22290.12379316932728493No Hit
TAGCAGATCTCTGCAGCTCAAGCCTCATTTCATCCTGCTCAGC21920.1217382804690034No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA21690.12046091712466622No Hit
GCCAGGGACTTCTGCAGAAACTGACGGAGTCTGGGGTCCGAGG21580.11985000422085278No Hit
GAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTGATATGGAC21480.11929462885374967No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20860.11585130157771034No Hit
CGGCAGGACAGCATCTCGGCGCCGCGAAAGCCGAGCTGGAGAG20800.11551807635744847No Hit
CTCCAGCTCGGCTTTCGCGGCGCCGAGATGCTGTCCTGCCGCC20700.11496270099034536No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG20600.11440732562324224No Hit
GATCTGCTAACTCAAACCCGGCTATGGCACCCCGAGAACGCAA20520.11396302532955974No Hit
CGCCTAGAGTTTGACCAGCCACTCTCCAGCTCGGCTTTCGCGG20450.11357426257258756No Hit
CCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGGCTGCTGCC20170.11201921154469882No Hit
ATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTGCG19900.11051969805352041No Hit
CTGCAGCTCAAGCCTCATTTCATCCTGCTCAGCAGCCTGGGAC19510.10835373412181824No Hit
ATGCTGTCCTGCCGCCTCCAGTGCGCGCTGGCTGCGCTGTCCA19500.10829819658510795No Hit
AGTCTGGGGTCCGAGGGAGCGCCGGTGACACAGCCCAGGGCCA19450.10802050890155639No Hit
GTATTGTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCC19430.10790943382813577No Hit
GTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAGCCAGGGAC19140.10629884526353672No Hit
GTTCGGAGCCATCGCTGCTGCCTGCTGATCCGCGCCTAGAGTT19010.10557685728630267No Hit
GCCATAGCCGGGTTTGAGTTAGCAGATCTCTGCAGCTCAAGCC18500.10274444291407676No Hit
GCTCAGCAGCCTGGGACAGATCTTCAGGTTCCAGGGCATCATT18280.10152261710644991No Hit
GTGTGGGGGCGAGGGATCAGAGGTCTGATATGGACAATACTAG18200.10107831681276742No Hit
GTCCTGCCGCCTCCAGTGCGCGCTGGCTGCGCTGTCCATCGTC18140.10074509159250554No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20250.018.5456771
TAGACCG609.2395843E-418.55
TATTAGA2000.018.4999982
TTAACGG1502.5102054E-1017.26666635
TTCGGTC803.383898E-416.187527
CTAACCG1151.2430755E-616.08695621
CAGTCGG2450.015.85714310
GTAAACG1403.4762706E-815.85714327
AATTTCG1557.215931E-915.516129528
ATTTCGG1557.215931E-915.516129529
TAACCGT1101.4526973E-515.13636422
ACGCTAT1101.4526973E-515.13636410
TATACGC1101.4526973E-515.1363647
CTATACT3200.015.031254
GTCCTAA3150.014.6825391
TCTTATA13050.014.60153237
ATACTTC1651.6465492E-814.5757576
GCTATCC1152.211109E-514.4782613
CGGTACC1152.211109E-514.47826114
TAACGGC1803.3342076E-914.38888836
Produced by FastQC (version 0.11.5)