##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630727.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1800584
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.06376597814931	33.0	31.0	34.0	30.0	34.0
2	32.24740306478343	34.0	31.0	34.0	30.0	34.0
3	32.366325592141216	34.0	31.0	34.0	30.0	34.0
4	35.94199159828145	37.0	35.0	37.0	35.0	37.0
5	35.89876229045687	37.0	35.0	37.0	35.0	37.0
6	35.96287315670916	37.0	35.0	37.0	35.0	37.0
7	35.90200235034855	37.0	35.0	37.0	35.0	37.0
8	35.89987470731718	37.0	35.0	37.0	35.0	37.0
9	37.638376215716676	39.0	37.0	39.0	35.0	39.0
10	37.5357111914801	39.0	37.0	39.0	35.0	39.0
11	37.625453741674924	39.0	37.0	39.0	35.0	39.0
12	37.548069404148876	39.0	37.0	39.0	35.0	39.0
13	37.603725235812384	39.0	37.0	39.0	35.0	39.0
14	38.931317283725726	40.0	38.0	41.0	36.0	41.0
15	38.93852216836315	40.0	38.0	41.0	36.0	41.0
16	38.92035750623131	40.0	38.0	41.0	36.0	41.0
17	38.86116948723303	40.0	38.0	41.0	35.0	41.0
18	38.84373181145673	40.0	38.0	41.0	35.0	41.0
19	38.887425413088195	40.0	38.0	41.0	35.0	41.0
20	38.86262068306727	40.0	38.0	41.0	35.0	41.0
21	38.81554873307771	40.0	38.0	41.0	35.0	41.0
22	38.76064710116273	40.0	38.0	41.0	35.0	41.0
23	38.72441163533609	40.0	38.0	41.0	35.0	41.0
24	38.67895305078797	40.0	38.0	41.0	34.0	41.0
25	38.630934741172865	40.0	38.0	41.0	34.0	41.0
26	38.5038965135756	40.0	38.0	41.0	34.0	41.0
27	38.385319429696146	40.0	38.0	41.0	34.0	41.0
28	38.30024203258498	40.0	38.0	41.0	34.0	41.0
29	38.22517749796732	40.0	38.0	41.0	34.0	41.0
30	38.13901156513664	40.0	38.0	41.0	33.0	41.0
31	38.07500622020411	40.0	38.0	41.0	33.0	41.0
32	38.01613643129118	40.0	37.0	41.0	33.0	41.0
33	37.943624957236096	40.0	37.0	41.0	33.0	41.0
34	37.8677895616089	40.0	37.0	41.0	33.0	41.0
35	37.8296591550297	40.0	37.0	41.0	33.0	41.0
36	37.788449747415285	40.0	37.0	41.0	33.0	41.0
37	37.6482452359901	40.0	37.0	41.0	33.0	41.0
38	37.54646103708575	40.0	37.0	41.0	32.0	41.0
39	37.46370899663665	40.0	36.0	41.0	32.0	41.0
40	37.294405592852094	40.0	36.0	41.0	32.0	41.0
41	37.23242847876023	39.0	36.0	41.0	31.0	41.0
42	37.12066918288733	39.0	36.0	41.0	31.0	41.0
43	36.30399026093756	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	6.0
10	3.0
11	5.0
12	0.0
13	1.0
14	0.0
15	4.0
16	6.0
17	14.0
18	55.0
19	90.0
20	164.0
21	349.0
22	679.0
23	1157.0
24	2148.0
25	3520.0
26	5547.0
27	8384.0
28	12213.0
29	17049.0
30	23573.0
31	31296.0
32	40798.0
33	52840.0
34	70634.0
35	95829.0
36	138589.0
37	221399.0
38	410187.0
39	664043.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.8195229992047	18.158164240046563	10.8023841153759	25.219928645372836
2	19.552434099158937	20.150017994161896	34.01868504885081	26.27886285782835
3	21.082160010307767	21.47975323561689	27.96831472455603	29.46977202951931
4	13.673063850395206	15.012573698311215	33.57605088126963	37.738311570023946
5	15.81881211873481	35.52147525469515	33.2662069639628	15.393505662607243
6	37.64095426817077	33.12791849755413	15.226115526962364	14.00501170731274
7	32.12685439835076	28.64981583752827	19.840729452222167	19.382600311898806
8	28.220121916000586	32.668234306202876	19.341224847049624	19.770418930746914
9	27.001683898113054	12.463845063601587	18.72598001537279	41.80849102291256
10	16.94072589781982	25.980626285693976	32.85173032749375	24.226917488992463
11	37.45718055919635	21.151748543805788	20.041941947723625	21.349128949274235
12	22.37290790099212	24.02414994246311	28.17741355027036	25.425528606274412
13	31.368322721961317	18.84499695654299	24.476558716505313	25.310121604990382
14	23.95456140896509	19.769030492329158	24.141000919701607	32.13540717900415
15	25.452908611872594	27.248992549084072	22.193132894660845	25.10496594438249
16	27.737389646914558	24.617512984676083	22.85669538327565	24.788401985133714
17	24.89125750312121	25.282019611414963	23.906299289563833	25.9204235959
18	24.310001643911086	23.586458615649146	26.308242214748105	25.795297525691662
19	26.95630973062073	24.49760744291852	24.814060327093877	23.732022499366874
20	26.67612285791721	24.083686181816567	24.75913370328738	24.481057256978847
21	25.293127118757024	24.503272271662972	24.631952744220765	25.57164786535924
22	26.807302519626962	23.168483114367337	24.849493275515055	25.174721090490642
23	23.85798163262586	24.261683986973114	25.451797861138388	26.42853651926264
24	25.226370999631232	24.886592350037542	24.432628524967456	25.45440812536377
25	25.3511638446193	24.50499393530099	25.010829819658507	25.133012400421194
26	26.4326462969792	24.650169056261745	23.672819485233678	25.244365161525373
27	26.45774926357226	23.86164711004874	23.515092880976393	26.165510745402603
28	25.179164093427463	24.58324632452582	25.075586587462734	25.162002994583982
29	25.72265442767458	25.036543699155384	25.390651033220337	23.850150839949706
30	25.70799251798305	24.117453004136436	24.120563106192215	26.053991371688294
31	24.3747028741786	23.961947901347564	25.17422125266025	26.48912797181359
32	24.164604372803492	24.745804694476902	24.750303234950437	26.33928769776917
33	25.075031212095634	23.612672332976413	25.117850652899282	26.194445802028675
34	26.21493915307478	24.129226961919024	25.282852674465616	24.37298121054058
35	26.345285751733883	23.861758185122163	24.194039267259956	25.598916795884
36	24.5384275324006	24.30472557792361	23.95372834591444	27.203118543761356
37	24.737862826727326	23.83326742878977	26.452695347731627	24.976174396751276
38	24.483723058740942	23.12394200992567	25.15461650220151	27.23771842913188
39	24.096182127576387	24.322608664744326	26.62830503880963	24.952904168869654
40	25.771083159685972	23.14254708472362	25.478122653539074	25.608247102051333
41	23.34020517787562	24.17349037867714	26.67512318225642	25.811181261190814
42	23.750627574164827	24.963234150697776	25.939806196211894	25.346332078925503
43	24.481057256978847	23.07440252718007	25.693330608291532	26.751209607549555
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	80.0
1	105.5
2	131.0
3	440.0
4	749.0
5	749.0
6	1014.0
7	1279.0
8	1276.5
9	1274.0
10	1769.5
11	2265.0
12	2265.0
13	3837.0
14	5409.0
15	7382.0
16	9355.0
17	9268.5
18	9182.0
19	9182.0
20	11174.0
21	13166.0
22	13249.0
23	13332.0
24	15509.5
25	17687.0
26	17687.0
27	19918.0
28	22149.0
29	25592.5
30	29036.0
31	33601.0
32	38166.0
33	38166.0
34	51399.0
35	64632.0
36	69491.0
37	74350.0
38	76174.0
39	77998.0
40	77998.0
41	81200.0
42	84402.0
43	92026.5
44	99651.0
45	99555.0
46	99459.0
47	99459.0
48	100716.0
49	101973.0
50	109931.5
51	117890.0
52	132089.0
53	146288.0
54	146288.0
55	144851.5
56	143415.0
57	129891.5
58	116368.0
59	116786.5
60	117205.0
61	117205.0
62	112808.0
63	108411.0
64	88656.0
65	68901.0
66	63198.5
67	57496.0
68	57496.0
69	53131.5
70	48767.0
71	50601.5
72	52436.0
73	46128.5
74	39821.0
75	39821.0
76	23668.0
77	7515.0
78	5887.0
79	4259.0
80	3452.0
81	2645.0
82	2645.0
83	2062.0
84	1479.0
85	1209.0
86	939.0
87	795.0
88	651.0
89	651.0
90	483.0
91	315.0
92	178.0
93	41.0
94	27.0
95	13.0
96	13.0
97	8.5
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1800584.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.73088714780165
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.88733811357076	40.9947884292393
2	8.423614670308066	8.041332024100816
3	2.1741682042077346	3.1132493158613386
4	0.9133760382413685	1.743849944192197
5	0.524449063478358	1.2516209531827887
6	0.33657316311321556	0.9638961399321319
7	0.23890815567022605	0.7982308751889492
8	0.17095623283606498	0.6527914125369216
9	0.12801619831958042	0.549929404357424
>10	0.9550150150531521	9.151092932427908
>50	0.11564230745695941	3.8647570287954167
>100	0.1017913317860201	9.947747151421112
>500	0.015426351885792413	5.2557162342376635
>1k	0.014374555166306568	12.7613163422998
>5k	3.5059890649528214E-4	0.9096818122261495
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG	5927	0.32917098008201784	No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT	5278	0.2931271187570255	No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	5103	0.28340804983272094	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	4855	0.26963474072856364	No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA	4468	0.24814171402167298	No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC	4375	0.24297672310761398	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4105	0.22798158819582978	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3777	0.20976527615484755	No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT	3532	0.19615857966082118	No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC	3505	0.19465906616964274	No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA	3464	0.19238202716451996	No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA	3389	0.18821671191124656	No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC	3385	0.18799456176440535	No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG	3343	0.18566198522257224	No Hit
GCCAAGAAGTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAG	3324	0.1846067720250763	No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT	3306	0.1836070963642907	No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC	3079	0.17100007553104993	No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC	3068	0.1703891626272365	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2986	0.16583508461699092	No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG	2965	0.1646687963460744	No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC	2964	0.16461325880936406	No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA	2964	0.16461325880936406	No Hit
GTTCCTGCTTCCCCGCGGCAGCAGCCAGGGACTTCTGCAGAAA	2873	0.1595593429687257	No Hit
GTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCA	2866	0.15917058021175354	No Hit
CCCTCGGACCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGG	2654	0.14739662242916743	No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC	2499	0.1387883042390691	No Hit
GATCAGCAGGCAGCAGCGATGGCTCCGAACCTCGCTCCTAAAG	2263	0.12568144557543554	No Hit
GAGTTTGACCAGCCACTCTCCAGCTCGGCTTTCGCGGCGCCGA	2250	0.12495945759820148	No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT	2244	0.1246262323779396	No Hit
GGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTG	2229	0.12379316932728493	No Hit
TAGCAGATCTCTGCAGCTCAAGCCTCATTTCATCCTGCTCAGC	2192	0.1217382804690034	No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA	2169	0.12046091712466622	No Hit
GCCAGGGACTTCTGCAGAAACTGACGGAGTCTGGGGTCCGAGG	2158	0.11985000422085278	No Hit
GAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTGATATGGAC	2148	0.11929462885374967	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2086	0.11585130157771034	No Hit
CGGCAGGACAGCATCTCGGCGCCGCGAAAGCCGAGCTGGAGAG	2080	0.11551807635744847	No Hit
CTCCAGCTCGGCTTTCGCGGCGCCGAGATGCTGTCCTGCCGCC	2070	0.11496270099034536	No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG	2060	0.11440732562324224	No Hit
GATCTGCTAACTCAAACCCGGCTATGGCACCCCGAGAACGCAA	2052	0.11396302532955974	No Hit
CGCCTAGAGTTTGACCAGCCACTCTCCAGCTCGGCTTTCGCGG	2045	0.11357426257258756	No Hit
CCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGGCTGCTGCC	2017	0.11201921154469882	No Hit
ATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTGCG	1990	0.11051969805352041	No Hit
CTGCAGCTCAAGCCTCATTTCATCCTGCTCAGCAGCCTGGGAC	1951	0.10835373412181824	No Hit
ATGCTGTCCTGCCGCCTCCAGTGCGCGCTGGCTGCGCTGTCCA	1950	0.10829819658510795	No Hit
AGTCTGGGGTCCGAGGGAGCGCCGGTGACACAGCCCAGGGCCA	1945	0.10802050890155639	No Hit
GTATTGTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCC	1943	0.10790943382813577	No Hit
GTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAGCCAGGGAC	1914	0.10629884526353672	No Hit
GTTCGGAGCCATCGCTGCTGCCTGCTGATCCGCGCCTAGAGTT	1901	0.10557685728630267	No Hit
GCCATAGCCGGGTTTGAGTTAGCAGATCTCTGCAGCTCAAGCC	1850	0.10274444291407676	No Hit
GCTCAGCAGCCTGGGACAGATCTTCAGGTTCCAGGGCATCATT	1828	0.10152261710644991	No Hit
GTGTGGGGGCGAGGGATCAGAGGTCTGATATGGACAATACTAG	1820	0.10107831681276742	No Hit
GTCCTGCCGCCTCCAGTGCGCGCTGGCTGCGCTGTCCATCGTC	1814	0.10074509159250554	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.887627569721824E-4	0.0
3	0.0	0.0	0.0	7.775255139443648E-4	0.0
4	0.0	0.0	0.0	0.001666126101309353	0.0
5	0.0	0.0	0.0	0.0018882762481506	0.0
6	0.0	0.0	0.0	0.0018882762481506	0.0
7	0.0	0.0	0.0	0.002165963931702159	0.0
8	0.0	5.553753671031177E-5	0.0	0.002277039005122782	0.0
9	0.0	5.553753671031177E-5	0.0	0.003554402349459953	0.0
10	5.553753671031177E-5	5.553753671031177E-5	0.0	0.0054982161343208645	0.0
11	5.553753671031177E-5	5.553753671031177E-5	0.0	0.005831441354582735	0.0
12	5.553753671031177E-5	5.553753671031177E-5	0.0	0.006275741648265229	0.0
13	5.553753671031177E-5	5.553753671031177E-5	0.0	0.006553429331816788	0.0
14	5.553753671031177E-5	5.553753671031177E-5	0.0	0.0068311170153683475	0.0
15	5.553753671031177E-5	5.553753671031177E-5	0.0	0.007775255139443647	0.0
16	5.553753671031177E-5	5.553753671031177E-5	0.0	0.008997080947070506	0.0
17	5.553753671031177E-5	5.553753671031177E-5	0.0	0.010996432268641729	0.0
18	5.553753671031177E-5	5.553753671031177E-5	0.0	0.011885032856006717	0.0
19	5.553753671031177E-5	5.553753671031177E-5	0.0	0.012551483296530459	0.0
20	5.553753671031177E-5	5.553753671031177E-5	0.0	0.01382884664086763	0.0
21	5.553753671031177E-5	5.553753671031177E-5	0.0	0.01571712288901823	0.0
22	5.553753671031177E-5	5.553753671031177E-5	0.0	0.019327062775188496	0.0
23	5.553753671031177E-5	5.553753671031177E-5	0.0	0.024436516152537176	0.0
24	5.553753671031177E-5	5.553753671031177E-5	0.0	0.0316008583881674	0.0
25	5.553753671031177E-5	5.553753671031177E-5	0.0	0.03432219768697267	0.0
26	5.553753671031177E-5	5.553753671031177E-5	0.0	0.03904288830734917	0.0
27	5.553753671031177E-5	5.553753671031177E-5	0.0	0.047928894180999056	0.0
28	1.666126101309353E-4	5.553753671031177E-5	0.0	0.07253202294366716	0.0
29	1.666126101309353E-4	5.553753671031177E-5	0.0	0.11090846081049259	0.0
30	1.666126101309353E-4	5.553753671031177E-5	0.0	0.15883735499149165	0.0
31	1.666126101309353E-4	5.553753671031177E-5	0.0	0.36049415078663366	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2025	0.0	18.545677	1
TAGACCG	60	9.2395843E-4	18.5	5
TATTAGA	200	0.0	18.499998	2
TTAACGG	150	2.5102054E-10	17.266666	35
TTCGGTC	80	3.383898E-4	16.1875	27
CTAACCG	115	1.2430755E-6	16.086956	21
CAGTCGG	245	0.0	15.857143	10
GTAAACG	140	3.4762706E-8	15.857143	27
AATTTCG	155	7.215931E-9	15.5161295	28
ATTTCGG	155	7.215931E-9	15.5161295	29
TAACCGT	110	1.4526973E-5	15.136364	22
ACGCTAT	110	1.4526973E-5	15.136364	10
TATACGC	110	1.4526973E-5	15.136364	7
CTATACT	320	0.0	15.03125	4
GTCCTAA	315	0.0	14.682539	1
TCTTATA	1305	0.0	14.601532	37
ATACTTC	165	1.6465492E-8	14.575757	6
GCTATCC	115	2.211109E-5	14.478261	3
CGGTACC	115	2.211109E-5	14.478261	14
TAACGGC	180	3.3342076E-9	14.388888	36
>>END_MODULE