FastQCFastQC Report
Fri 10 Feb 2017
ERR1630726.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630726.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2054732
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT52140.25375572094073584No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT48850.23774390042107682No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT41700.20294617497561726No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG39180.19068180181162311No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG30690.14936254460435716No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT30220.1470751416729773No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA28240.1374388484726962No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27570.13417808259179298No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC26410.1285325774845576No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC25150.12240039090256052No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT21810.10614522964552067No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA20820.10132708304538013No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTATCG250.005497338729.65
TCGCCAT6750.018.9111113
AACCGAT650.001580507117.0769217
GTATTAC1352.2220775E-816.4444451
GCGTTAT2550.015.9607841
TACACCG700.002593425615.8571435
TCTAGCG2250.015.62222228
GGTATCA30400.015.5180921
GTATTAT2150.015.4883721
GTCGCCA8350.015.28742512
GTAGAAC10450.014.8708133
GTCTACG1001.0940296E-414.81
CGCATGA750.0041058714.79999937
AGAGGTC9550.014.5287958
TATACTG2201.8189894E-1114.2954545
CAAGACG3500.014.2714294
AAGACGG3250.014.2307695
ATACCGC3250.014.23076927
TACCGCA3000.014.18333228
TTGGCCG8950.014.05586631
Produced by FastQC (version 0.11.5)