##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630726.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2054732
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.14038424475795	33.0	31.0	34.0	30.0	34.0
2	32.32011376666154	34.0	31.0	34.0	30.0	34.0
3	32.415434226945415	34.0	31.0	34.0	30.0	34.0
4	35.977530402991725	37.0	35.0	37.0	35.0	37.0
5	35.930371454768796	37.0	35.0	37.0	35.0	37.0
6	36.00717952511568	37.0	35.0	37.0	35.0	37.0
7	35.9606308754621	37.0	35.0	37.0	35.0	37.0
8	35.954671460803645	37.0	35.0	37.0	35.0	37.0
9	37.68667300650401	39.0	37.0	39.0	35.0	39.0
10	37.599470879900636	39.0	37.0	39.0	35.0	39.0
11	37.690236488262215	39.0	37.0	39.0	35.0	39.0
12	37.640013393474185	39.0	37.0	39.0	35.0	39.0
13	37.665405999419875	39.0	37.0	39.0	35.0	39.0
14	39.001111093806884	40.0	38.0	41.0	36.0	41.0
15	39.020400227377586	40.0	38.0	41.0	36.0	41.0
16	38.98620501359788	40.0	38.0	41.0	36.0	41.0
17	38.96722054263038	40.0	38.0	41.0	36.0	41.0
18	38.93763517577961	40.0	38.0	41.0	36.0	41.0
19	39.01362270116005	40.0	39.0	41.0	36.0	41.0
20	38.9807609946212	40.0	39.0	41.0	35.0	41.0
21	38.938059075344135	40.0	38.0	41.0	35.0	41.0
22	38.883560970481795	40.0	38.0	41.0	35.0	41.0
23	38.83300206547618	40.0	38.0	41.0	35.0	41.0
24	38.794076794443264	40.0	38.0	41.0	35.0	41.0
25	38.74608026740227	40.0	38.0	41.0	35.0	41.0
26	38.62859438603185	40.0	38.0	41.0	34.0	41.0
27	38.51512849364297	40.0	38.0	41.0	34.0	41.0
28	38.43034030715441	40.0	38.0	41.0	34.0	41.0
29	38.37334795973392	40.0	38.0	41.0	34.0	41.0
30	38.30325171360547	40.0	38.0	41.0	34.0	41.0
31	38.26358279327912	40.0	38.0	41.0	34.0	41.0
32	38.17829283819009	40.0	38.0	41.0	34.0	41.0
33	38.11848309171221	40.0	38.0	41.0	33.0	41.0
34	38.0728567034533	40.0	38.0	41.0	33.0	41.0
35	38.01985173735553	40.0	38.0	41.0	33.0	41.0
36	37.996618537113356	40.0	38.0	41.0	33.0	41.0
37	37.88675360095623	40.0	37.0	41.0	33.0	41.0
38	37.74005758415209	40.0	37.0	41.0	33.0	41.0
39	37.700953701017944	40.0	37.0	41.0	33.0	41.0
40	37.55649787904213	40.0	37.0	41.0	32.0	41.0
41	37.511871134532385	40.0	37.0	41.0	32.0	41.0
42	37.39874056567961	40.0	37.0	41.0	32.0	41.0
43	36.60630048103597	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	2.0
10	0.0
11	6.0
12	7.0
13	3.0
14	4.0
15	7.0
16	6.0
17	19.0
18	42.0
19	89.0
20	208.0
21	420.0
22	766.0
23	1337.0
24	2304.0
25	3624.0
26	5617.0
27	8616.0
28	12701.0
29	18116.0
30	25101.0
31	33241.0
32	43262.0
33	56998.0
34	76324.0
35	104100.0
36	148059.0
37	232919.0
38	450627.0
39	830203.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.58125536566326	19.128090670705475	12.164798134257898	25.125855829373368
2	18.379963907701832	21.519594769536855	35.360718575463856	24.739722747297456
3	20.278411004452163	23.77380602433797	29.811673736526224	26.136109234683648
4	14.686148850555695	15.460653749491419	34.1680569534129	35.68514044653999
5	13.918165483381776	37.78239692573046	34.42818820167302	13.871249389214748
6	34.89997722330698	34.39879264059741	15.980040219357075	14.721189916738533
7	28.533550847507122	30.777785132075618	21.662095105347074	19.02656891507019
8	26.299682878351042	34.401566725003555	20.417650574381476	18.881099822263923
9	26.354969893883972	14.308532694288111	19.75727248127736	39.57922493055055
10	16.41931891847696	27.591140839778618	33.368049945199665	22.621490296544756
11	34.8790985880397	22.16035959920807	21.92573046022547	21.034811352526752
12	21.275475341796398	25.961293248949254	29.112701802473513	23.650529606780836
13	29.3009988650588	20.41224841001162	25.978959786483102	24.307792938446475
14	22.85329668297374	21.434668852190946	25.160312877786495	30.551721587048824
15	25.372944014109873	28.255023039501015	22.97725445459554	23.394778491793577
16	25.22027203547713	26.9607909936673	23.929592764409175	23.88934420644639
17	23.636561848455177	27.005127676018088	25.347977254454594	24.010333221072138
18	22.565229918062307	25.753577595520973	27.442410981091452	24.238781505325267
19	24.151324844310594	26.013319498601277	27.126311363233746	22.70904429385438
20	24.504850267577474	25.000827358507095	27.325753431591078	23.168568942324352
21	24.133414966039364	26.19704175532381	25.833490693676843	23.836052584959987
22	24.240484890486936	25.553794850131307	26.00524058612023	24.200479673261523
23	23.312918667738664	26.27953426529591	26.45147883032921	23.956068236636213
24	24.530644385739844	26.28250302229196	26.115522608301227	23.07132998366697
25	24.28598960837715	25.563187802594207	26.4269987521487	23.723823836879944
26	24.196829562200815	26.061500964602686	26.16287671579554	23.578792757400965
27	24.49462995660748	25.359949618733737	25.849843191228832	24.295577233429956
28	23.36543159886545	25.78219446623696	26.794394597446285	24.057979337451307
29	23.38558021192058	26.40874819684514	26.549545147493685	23.656126443740593
30	22.90624762742781	27.29767191049733	26.500292982247807	23.295787479827055
31	23.91397028906933	26.311265897450376	26.30065624130057	23.47410757217973
32	22.95817654078488	26.346258295485736	26.29520540878324	24.400359754946145
33	23.192805679767485	25.915009840699422	26.685815960426957	24.206368519106142
34	23.699830440174193	25.85339596599459	26.559619454021256	23.88715413980996
35	23.85148038770993	25.99701566919676	26.609212296299468	23.54229164679384
36	23.271988755711206	26.525795091525318	26.504332438488326	23.697883714275147
37	24.428003262712608	25.431345791081274	26.078340143629436	24.062310802576686
38	23.441548581518173	25.82463309083618	27.20865786876342	23.52516045888223
39	23.64274270318465	25.644609613321833	26.95519415670754	23.757453526785973
40	23.127103680674658	25.13816887068484	27.814138291514418	23.920589157126088
41	22.350068038070173	25.923575434655227	28.05835505554982	23.66800147172478
42	23.49722494223091	25.000876026654574	27.66691714539901	23.834981885715507
43	22.56532725435726	25.054264984435925	27.726632962352266	24.653774798854545
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	108.0
1	121.0
2	134.0
3	497.0
4	860.0
5	860.0
6	1212.0
7	1564.0
8	1617.5
9	1671.0
10	2514.0
11	3357.0
12	3357.0
13	5395.5
14	7434.0
15	11010.5
16	14587.0
17	14797.0
18	15007.0
19	15007.0
20	16710.0
21	18413.0
22	19604.0
23	20795.0
24	23470.5
25	26146.0
26	26146.0
27	30391.0
28	34636.0
29	46099.0
30	57562.0
31	61368.5
32	65175.0
33	65175.0
34	71462.5
35	77750.0
36	82920.0
37	88090.0
38	103137.0
39	118184.0
40	118184.0
41	126631.5
42	135079.0
43	127600.5
44	120122.0
45	127857.0
46	135592.0
47	135592.0
48	142095.0
49	148598.0
50	153277.5
51	157957.0
52	168005.0
53	178053.0
54	178053.0
55	156399.5
56	134746.0
57	126290.0
58	117834.0
59	105774.5
60	93715.0
61	93715.0
62	87369.0
63	81023.0
64	72024.5
65	63026.0
66	54763.5
67	46501.0
68	46501.0
69	39434.0
70	32367.0
71	28111.0
72	23855.0
73	19040.0
74	14225.0
75	14225.0
76	11281.0
77	8337.0
78	6925.5
79	5514.0
80	4269.0
81	3024.0
82	3024.0
83	2289.5
84	1555.0
85	1274.0
86	993.0
87	852.0
88	711.0
89	711.0
90	545.0
91	379.0
92	210.5
93	42.0
94	25.5
95	9.0
96	9.0
97	5.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2054732.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.92653813070288
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.18624166399216	43.38240384312567
2	9.045492268544498	9.21311213850019
3	2.3291010402267642	3.558391588061041
4	0.9956991079500419	2.028300343508986
5	0.5452368925689492	1.3883513699839272
6	0.3378984202188031	1.0324798068946288
7	0.22550466673317407	0.8038920406326904
8	0.16821593502911572	0.685332418356168
9	0.11809225375868934	0.541262669758428
>10	0.7923504654239361	7.7962765875360995
>50	0.1048435290416173	3.7965794008719405
>100	0.12717425777423672	14.234644319187456
>500	0.01792046136511906	6.092298056682211
>1k	0.00613320602870385	5.192213519265102
>5k	9.583134419849765E-5	0.25446189763556953
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5214	0.25375572094073584	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4885	0.23774390042107682	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4170	0.20294617497561726	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	3918	0.19068180181162311	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	3069	0.14936254460435716	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	3022	0.1470751416729773	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	2824	0.1374388484726962	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2757	0.13417808259179298	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	2641	0.1285325774845576	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	2515	0.12240039090256052	No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT	2181	0.10614522964552067	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	2082	0.10132708304538013	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.8668147476167206E-5	0.0
2	0.0	4.8668147476167206E-5	0.0	0.0012167036869041802	0.0
3	0.0	9.733629495233441E-5	0.0	0.0020440621939990227	0.0
4	0.0	9.733629495233441E-5	0.0	0.004088124387998045	0.0
5	0.0	2.920088848570033E-4	0.0	0.004623474010235885	0.0
6	0.0	2.920088848570033E-4	0.0	0.004866814747616721	0.0
7	0.0	2.920088848570033E-4	0.0	0.005353496222378393	0.0
8	0.0	2.920088848570033E-4	0.0	0.005694173254711564	0.0
9	0.0	2.920088848570033E-4	0.0	0.009684961347757275	0.0
10	4.8668147476167206E-5	2.920088848570033E-4	0.0	0.01411376276808849	0.0
11	4.8668147476167206E-5	2.920088848570033E-4	0.0	0.015963152372182845	0.0
12	4.8668147476167206E-5	2.920088848570033E-4	0.0	0.017033851616658522	0.0
13	4.8668147476167206E-5	2.920088848570033E-4	0.0	0.018007214566181866	0.0
14	4.8668147476167206E-5	2.920088848570033E-4	0.0	0.018931909368229045	0.0
15	4.8668147476167206E-5	2.920088848570033E-4	0.0	0.020489290087466396	0.0
16	4.8668147476167206E-5	2.920088848570033E-4	0.0	0.022776693018846255	0.0
17	4.8668147476167206E-5	2.920088848570033E-4	0.0	0.02579411816236862	0.0
18	4.8668147476167206E-5	3.8934517980933765E-4	0.0	0.027935516651319978	0.0
19	4.8668147476167206E-5	4.3801332728550486E-4	0.0	0.030904273647366177	0.0
20	4.8668147476167206E-5	4.3801332728550486E-4	0.0	0.033337681021174535	0.0
21	4.8668147476167206E-5	4.3801332728550486E-4	0.0	0.03684178763945858	0.0
22	4.8668147476167206E-5	4.3801332728550486E-4	0.0	0.043119978663884145	0.0
23	4.8668147476167206E-5	4.3801332728550486E-4	0.0	0.05212358594697508	0.0
24	4.8668147476167206E-5	4.8668147476167207E-4	0.0	0.06380394134125521	0.0
25	4.8668147476167206E-5	4.8668147476167207E-4	0.0	0.06774606128682475	0.0
26	4.8668147476167206E-5	4.8668147476167207E-4	0.0	0.07344023454153632	0.0
27	4.8668147476167206E-5	4.8668147476167207E-4	0.0	0.08078912481043757	0.0
28	4.8668147476167206E-5	4.8668147476167207E-4	0.0	0.09519489646338306	0.0
29	4.8668147476167206E-5	5.353496222378393E-4	0.0	0.11957763834894283	0.0
30	4.8668147476167206E-5	5.353496222378393E-4	0.0	0.15729545264297243	0.0
31	4.8668147476167206E-5	5.353496222378393E-4	0.0	0.3024725365643792	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTATCG	25	0.0054973387	29.6	5
TCGCCAT	675	0.0	18.91111	13
AACCGAT	65	0.0015805071	17.076921	7
GTATTAC	135	2.2220775E-8	16.444445	1
GCGTTAT	255	0.0	15.960784	1
TACACCG	70	0.0025934256	15.857143	5
TCTAGCG	225	0.0	15.622222	28
GGTATCA	3040	0.0	15.518092	1
GTATTAT	215	0.0	15.488372	1
GTCGCCA	835	0.0	15.287425	12
GTAGAAC	1045	0.0	14.870813	3
GTCTACG	100	1.0940296E-4	14.8	1
CGCATGA	75	0.00410587	14.799999	37
AGAGGTC	955	0.0	14.528795	8
TATACTG	220	1.8189894E-11	14.295454	5
CAAGACG	350	0.0	14.271429	4
AAGACGG	325	0.0	14.230769	5
ATACCGC	325	0.0	14.230769	27
TACCGCA	300	0.0	14.183332	28
TTGGCCG	895	0.0	14.055866	31
>>END_MODULE