FastQCFastQC Report
Fri 10 Feb 2017
ERR1630725.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630725.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1556040
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT79120.5084702192745687No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT76030.4886121179404128No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT61030.3922135677746073No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42750.2747358679725457No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAC551.9032199E-523.5454541
TAACACG609.238816E-418.54
CGTACAC953.6080473E-617.5263163
TCACGTA2100.016.73809625
CTTATAC13950.016.31182737
CGAACGA1151.24281E-616.08695616
TACTCCG2550.015.9607845
AGCGTAC700.002593046315.8571431
GTCTAAC700.002593046315.8571431
GCGTACA700.002593046315.8571432
GCTTTAT4850.015.6391751
GCAGTCG2250.015.6222229
GTCGAAG957.062242E-515.57894826
TCGGTGA2450.015.1020413
CGAAAGC2109.094947E-1214.976191519
TCCGGTC2251.8189894E-1214.88
TTGCGCG1001.093759E-414.79999918
ACGAACG1252.9601888E-614.79999915
TCTATGG5550.014.6666672
CAAGACG2650.014.66037754
Produced by FastQC (version 0.11.5)