##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630725.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1556040
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.114693709673276	33.0	31.0	34.0	30.0	34.0
2	32.284803732551865	34.0	31.0	34.0	30.0	34.0
3	32.391428240919254	34.0	31.0	34.0	30.0	34.0
4	35.9567453278836	37.0	35.0	37.0	35.0	37.0
5	35.91266355620678	37.0	35.0	37.0	35.0	37.0
6	35.990259890491245	37.0	35.0	37.0	35.0	37.0
7	35.93919436518341	37.0	35.0	37.0	35.0	37.0
8	35.944890876841214	37.0	35.0	37.0	35.0	37.0
9	37.66159738824195	39.0	37.0	39.0	35.0	39.0
10	37.57923703760829	39.0	37.0	39.0	35.0	39.0
11	37.67414141024652	39.0	37.0	39.0	35.0	39.0
12	37.63018688465592	39.0	37.0	39.0	35.0	39.0
13	37.6699403614303	39.0	37.0	39.0	35.0	39.0
14	38.968896043803504	40.0	38.0	41.0	36.0	41.0
15	38.980170175573896	40.0	38.0	41.0	36.0	41.0
16	38.94320133158531	40.0	38.0	41.0	36.0	41.0
17	38.940618493097865	40.0	38.0	41.0	36.0	41.0
18	38.936737487468186	40.0	38.0	41.0	36.0	41.0
19	38.99731048045037	40.0	38.0	41.0	35.0	41.0
20	38.96925143312511	40.0	38.0	41.0	35.0	41.0
21	38.92878460707951	40.0	38.0	41.0	35.0	41.0
22	38.85837639135241	40.0	38.0	41.0	35.0	41.0
23	38.811444435875686	40.0	38.0	41.0	35.0	41.0
24	38.760637258682294	40.0	38.0	41.0	35.0	41.0
25	38.70763476517313	40.0	38.0	41.0	35.0	41.0
26	38.57298848358654	40.0	38.0	41.0	34.0	41.0
27	38.448376005758206	40.0	38.0	41.0	34.0	41.0
28	38.35201151641346	40.0	38.0	41.0	34.0	41.0
29	38.2834894989846	40.0	38.0	41.0	34.0	41.0
30	38.18973226909334	40.0	38.0	41.0	34.0	41.0
31	38.13420991748284	40.0	37.0	41.0	34.0	41.0
32	38.02987969460939	40.0	37.0	41.0	33.0	41.0
33	37.94083506850723	40.0	37.0	41.0	33.0	41.0
34	37.869556052543636	40.0	37.0	41.0	33.0	41.0
35	37.802032724094495	40.0	37.0	41.0	33.0	41.0
36	37.750829027531424	40.0	37.0	41.0	33.0	41.0
37	37.60938793347215	40.0	37.0	41.0	33.0	41.0
38	37.458508778694636	40.0	37.0	41.0	32.0	41.0
39	37.3884315313231	40.0	36.0	41.0	32.0	41.0
40	37.197824606051256	40.0	36.0	41.0	31.0	41.0
41	37.1244800904861	40.0	36.0	41.0	31.0	41.0
42	36.97451993522017	40.0	36.0	41.0	31.0	41.0
43	36.20982301226189	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	6.0
10	5.0
11	6.0
12	8.0
13	5.0
14	3.0
15	6.0
16	2.0
17	9.0
18	39.0
19	90.0
20	177.0
21	316.0
22	654.0
23	1087.0
24	1962.0
25	3026.0
26	4688.0
27	7117.0
28	10603.0
29	14953.0
30	20425.0
31	26862.0
32	34786.0
33	45555.0
34	61216.0
35	82543.0
36	116533.0
37	185524.0
38	339631.0
39	598201.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.195007840415414	19.373216626821932	12.16022724351559	24.271548289247065
2	18.56269761702784	21.05299349631115	34.76652271149842	25.617786175162593
3	20.606218349142697	23.652862394282923	29.328166371044446	26.412752885529933
4	14.125536618595923	15.244466723220482	34.65367214210431	35.97632451607928
5	14.108891802267292	37.669275854091154	34.08588468162772	14.135947662013832
6	34.793450039844735	34.617940412842856	15.737513174468523	14.851096372843886
7	28.796624765430195	30.73744890876841	21.696293154417624	18.769633171383767
8	27.97903652862394	32.73964679571219	20.044728927276935	19.23658774838693
9	26.634276753810955	13.506208066630679	20.63822266779774	39.22129251176062
10	17.118454538443743	27.28078969692296	33.018302871391484	22.58245289324182
11	34.79184339734197	22.15810647541194	21.801688902598904	21.24836122464718
12	21.963252872676794	26.193735379553225	29.127978715200125	22.715033032569856
13	30.145818873550805	20.1976170278399	25.61605100125961	24.040513097349685
14	22.664584457983086	21.355556412431557	26.420914629443974	29.558944500141383
15	25.547222436441224	28.113287576154853	23.27478728053263	23.064702706871287
16	24.391596617053544	26.687938613403254	25.176602143903754	23.743862625639444
17	22.951787871777075	26.88472018714172	26.51840569651166	23.645086244569548
18	22.66413459808231	25.37788231664996	28.31039047839387	23.64759260687386
19	23.56160510012596	25.828256343024602	28.33712500963986	22.273013547209583
20	24.197128608519062	25.11702783990129	27.796393408909797	22.889450142669855
21	24.367175651011543	25.398190277884886	27.404629701035965	22.83000437006761
22	24.197578468419835	25.549150407444536	27.454756947122185	22.798514177013445
23	23.6551759594869	25.583918151204337	27.28695920413357	23.473946685175186
24	23.303771111282487	26.125613737436055	27.54093725097041	23.029677900311047
25	23.55916300352176	25.942713554921465	27.178992827947866	23.319130613608905
26	23.2190689185368	26.426120151152926	27.350517981542893	23.004292948767382
27	23.43821495591373	25.756985681602014	27.38258656589805	23.422212796586205
28	22.941312562659057	26.209416210380198	27.687463047222437	23.161808179738312
29	22.89253489627516	26.549060435464384	27.99805917585666	22.560345492403794
30	22.95847150458857	26.61049844477006	27.438304927893885	22.992725122747487
31	22.619662733605818	26.962288887175134	27.394025860517722	23.02402251870132
32	22.36125035346135	26.445335595485975	27.566514999614405	23.626899051438265
33	22.149109277396466	26.550667077967148	27.926595717333747	23.37362792730264
34	23.385774144623532	25.67607516516285	27.603532042878076	23.334618647335546
35	22.743117143518162	25.900362458548624	27.890671190971954	23.465849206961263
36	22.24351559085885	26.40118505951004	27.776471041875528	23.578828307755582
37	22.625510912315878	25.28823166499576	28.387573584226626	23.698683838461736
38	22.502763425104753	25.3561605100126	28.44830467083108	23.69277139405157
39	22.32526156139945	25.15417341456518	28.838204673401712	23.68236035063366
40	21.70304105292923	25.128402868820853	29.319233438729082	23.849322639520835
41	21.04978021130562	25.334760031875785	29.696858692578598	23.918601064239994
42	21.28672784761317	25.36766407033238	29.74878537826791	23.596822703786536
43	21.194827896455102	25.073391429526232	29.53792961620524	24.193851057813422
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	459.0
1	401.0
2	343.0
3	936.5
4	1530.0
5	1530.0
6	2070.5
7	2611.0
8	2583.0
9	2555.0
10	3676.5
11	4798.0
12	4798.0
13	8172.0
14	11546.0
15	15725.5
16	19905.0
17	19110.0
18	18315.0
19	18315.0
20	21047.0
21	23779.0
22	22434.0
23	21089.0
24	23623.0
25	26157.0
26	26157.0
27	29298.0
28	32439.0
29	36690.5
30	40942.0
31	45700.5
32	50459.0
33	50459.0
34	55995.0
35	61531.0
36	66880.5
37	72230.0
38	77023.0
39	81816.0
40	81816.0
41	85245.5
42	88675.0
43	90655.0
44	92635.0
45	94372.0
46	96109.0
47	96109.0
48	99093.0
49	102077.0
50	100893.5
51	99710.0
52	98195.5
53	96681.0
54	96681.0
55	94061.0
56	91441.0
57	88830.5
58	86220.0
59	84541.0
60	82862.0
61	82862.0
62	76235.5
63	69609.0
64	63018.0
65	56427.0
66	49076.5
67	41726.0
68	41726.0
69	36625.0
70	31524.0
71	26595.0
72	21666.0
73	16591.0
74	11516.0
75	11516.0
76	8780.0
77	6044.0
78	4806.0
79	3568.0
80	2869.5
81	2171.0
82	2171.0
83	1700.0
84	1229.0
85	999.0
86	769.0
87	679.0
88	589.0
89	589.0
90	411.5
91	234.0
92	135.5
93	37.0
94	26.0
95	15.0
96	15.0
97	8.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1556040.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.64437951493233
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.48438945909375	56.55374073825802
2	8.023756200753212	10.373814819535646
3	1.88749488959376	3.66047807926183
4	0.7372595643934052	1.906387483266439
5	0.42854106102561196	1.385138549333572
6	0.2544379809467749	0.9868791242001863
7	0.17513605533693133	0.7925093139564144
8	0.12422070697864701	0.6424136420432688
9	0.10234409168376657	0.5954373273524792
>10	0.6314826562193627	8.034784278177613
>50	0.07855091445951308	3.54529900010609
>100	0.06860291871152191	8.214952166766881
>500	0.002787842697569806	1.1840346341985037
>1k	6.969606743924515E-4	0.7327163663685458
>5k	2.9869743188247927E-4	1.3914144771745627
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7912	0.5084702192745687	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7603	0.4886121179404128	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6103	0.3922135677746073	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4275	0.2747358679725457	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.28531400221074E-4	0.0
2	0.0	0.0	0.0	3.2132850055268503E-4	0.0
3	0.0	0.0	0.0	5.14125600884296E-4	0.0
4	0.0	0.0	0.0	6.426570011053701E-4	0.0
5	1.28531400221074E-4	0.0	0.0	8.35454101436981E-4	0.0
6	1.28531400221074E-4	0.0	0.0	8.99719801547518E-4	0.0
7	1.28531400221074E-4	0.0	0.0	0.001028251201768592	0.0
8	1.28531400221074E-4	0.0	0.0	0.001156782601989666	0.0
9	1.28531400221074E-4	0.0	0.0	0.001670908202873962	0.0
10	1.28531400221074E-4	1.28531400221074E-4	0.0	0.002763425104753091	0.0
11	1.28531400221074E-4	1.28531400221074E-4	0.0	0.003470347805968998	0.0
12	1.28531400221074E-4	1.28531400221074E-4	0.0	0.003984473406853295	0.0
13	1.28531400221074E-4	1.28531400221074E-4	0.0	0.004562864707848127	0.0
14	1.28531400221074E-4	1.28531400221074E-4	0.0	0.005205521708953498	0.0
15	1.28531400221074E-4	1.28531400221074E-4	0.0	0.0061052415105010156	0.0
16	1.28531400221074E-4	1.28531400221074E-4	0.0	0.009382792216138403	0.0
17	1.28531400221074E-4	1.28531400221074E-4	0.0	0.014395516824760288	0.0
18	1.28531400221074E-4	1.28531400221074E-4	0.0	0.01593789362741318	0.0
19	1.28531400221074E-4	1.28531400221074E-4	0.0	0.01889411583249788	0.0
20	1.28531400221074E-4	1.28531400221074E-4	0.0	0.02075782113570345	0.0
21	1.28531400221074E-4	1.28531400221074E-4	0.0	0.02615613994498856	0.0
22	1.28531400221074E-4	1.28531400221074E-4	0.0	0.03586026066167965	0.0
23	1.28531400221074E-4	1.28531400221074E-4	0.0	0.053019202591193026	0.0
24	1.28531400221074E-4	1.28531400221074E-4	0.0	0.08001079663761856	0.0
25	1.9279710033161102E-4	1.28531400221074E-4	0.0	0.08727282075010925	0.0
26	1.9279710033161102E-4	1.28531400221074E-4	0.0	0.09993316367188504	0.0
27	2.57062800442148E-4	1.28531400221074E-4	0.0	0.11959846790570937	0.0
28	2.57062800442148E-4	1.28531400221074E-4	0.0	0.16194956427855325	0.0
29	2.57062800442148E-4	1.28531400221074E-4	0.0	0.22885015809362227	0.0
30	2.57062800442148E-4	1.28531400221074E-4	0.0	0.33463150047556617	0.0
31	2.57062800442148E-4	1.28531400221074E-4	0.0	0.6952263437957893	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGTAC	55	1.9032199E-5	23.545454	1
TAACACG	60	9.238816E-4	18.5	4
CGTACAC	95	3.6080473E-6	17.526316	3
TCACGTA	210	0.0	16.738096	25
CTTATAC	1395	0.0	16.311827	37
CGAACGA	115	1.24281E-6	16.086956	16
TACTCCG	255	0.0	15.960784	5
AGCGTAC	70	0.0025930463	15.857143	1
GTCTAAC	70	0.0025930463	15.857143	1
GCGTACA	70	0.0025930463	15.857143	2
GCTTTAT	485	0.0	15.639175	1
GCAGTCG	225	0.0	15.622222	9
GTCGAAG	95	7.062242E-5	15.578948	26
TCGGTGA	245	0.0	15.10204	13
CGAAAGC	210	9.094947E-12	14.9761915	19
TCCGGTC	225	1.8189894E-12	14.8	8
TTGCGCG	100	1.093759E-4	14.799999	18
ACGAACG	125	2.9601888E-6	14.799999	15
TCTATGG	555	0.0	14.666667	2
CAAGACG	265	0.0	14.6603775	4
>>END_MODULE