FastQCFastQC Report
Fri 10 Feb 2017
ERR1630723.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630723.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1567870
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT91460.5833391799064973No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT74060.47236059112043727No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT65380.4169988583237131No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51810.3304483152302168No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC21720.13853189358811635No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA21130.1347688264970948No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA19860.12666866513167546No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT18350.11703776461058632No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGTA500.007035996818.525
GGTATCA42400.018.1945761
TCGTTTA1351.1532393E-917.81481630
TCTAGCG1603.45608E-1117.3437528
GTACTAG904.4476183E-516.4444451
CTAGCGG1708.54925E-1116.3235329
TACACGT700.002593057715.8571435
TGCACCG855.3656986E-415.2352945
CAGTCGG2600.014.94230710
CGTTATT1752.239176E-914.7999992
TCTTATA13600.014.69117637
GCAGTCG2800.014.5357159
TTTCGGA2059.276846E-1114.43902530
GTTTTCG2059.276846E-1114.43902528
ACGTTCG908.276384E-414.3888887
TTAACGG908.276384E-414.38888835
CGCAATA1956.730261E-1014.2307736
ATAAGAC1304.4461576E-614.2307693
TTCGGAA2101.364242E-1014.09523831
GCGTTAT1854.9003575E-914.01
Produced by FastQC (version 0.11.5)