##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630723.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1567870
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.783288155267975	31.0	31.0	34.0	30.0	34.0
2	31.94260940001403	33.0	31.0	34.0	30.0	34.0
3	32.045109607301626	33.0	31.0	34.0	30.0	34.0
4	35.66866640729142	37.0	35.0	37.0	33.0	37.0
5	35.64903212638803	37.0	35.0	37.0	33.0	37.0
6	35.689969831682475	37.0	35.0	37.0	33.0	37.0
7	35.66381460197593	37.0	35.0	37.0	33.0	37.0
8	35.665675725666034	37.0	35.0	37.0	33.0	37.0
9	37.33658020116464	39.0	37.0	39.0	34.0	39.0
10	37.23186616237316	39.0	37.0	39.0	34.0	39.0
11	37.35190736476876	39.0	37.0	39.0	34.0	39.0
12	37.282004247801154	39.0	37.0	39.0	34.0	39.0
13	37.34380146313151	39.0	37.0	39.0	34.0	39.0
14	38.55147620657325	40.0	38.0	41.0	34.0	41.0
15	38.5148724065133	40.0	38.0	41.0	34.0	41.0
16	38.48382518958842	40.0	38.0	41.0	34.0	41.0
17	38.49253828442409	40.0	38.0	41.0	34.0	41.0
18	38.48365425704937	40.0	38.0	41.0	34.0	41.0
19	38.50823601446549	40.0	38.0	41.0	34.0	41.0
20	38.503148858004806	40.0	38.0	41.0	34.0	41.0
21	38.42977670342567	40.0	38.0	41.0	34.0	41.0
22	38.3592644798357	40.0	38.0	41.0	34.0	41.0
23	38.34411143781053	40.0	38.0	41.0	34.0	41.0
24	38.28121272809608	40.0	38.0	41.0	34.0	41.0
25	38.23737108306173	40.0	38.0	41.0	34.0	41.0
26	38.01393674220439	40.0	37.0	41.0	33.0	41.0
27	37.88295203046171	40.0	37.0	41.0	33.0	41.0
28	37.77870550492069	40.0	37.0	41.0	33.0	41.0
29	37.66404421284928	40.0	37.0	41.0	32.0	41.0
30	37.58027451255525	40.0	37.0	41.0	32.0	41.0
31	37.51376070720149	40.0	37.0	41.0	32.0	41.0
32	37.417366235721076	40.0	36.0	41.0	32.0	41.0
33	37.35776371765516	40.0	36.0	41.0	32.0	41.0
34	37.259963517383454	39.0	36.0	41.0	31.0	41.0
35	37.18669468769732	39.0	36.0	41.0	31.0	41.0
36	37.13164930765944	39.0	36.0	41.0	31.0	41.0
37	36.91343414951495	39.0	35.0	41.0	31.0	41.0
38	36.78314273504819	39.0	35.0	41.0	30.0	41.0
39	36.68129628094166	39.0	35.0	41.0	30.0	41.0
40	36.53804652171417	39.0	35.0	41.0	30.0	41.0
41	36.38912154706704	39.0	35.0	41.0	30.0	41.0
42	36.27266610114359	39.0	35.0	41.0	30.0	41.0
43	35.4308839380816	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	9.0
11	5.0
12	12.0
13	5.0
14	3.0
15	8.0
16	13.0
17	29.0
18	55.0
19	145.0
20	271.0
21	544.0
22	1020.0
23	1729.0
24	2894.0
25	4624.0
26	7202.0
27	10634.0
28	14792.0
29	20581.0
30	27172.0
31	35001.0
32	45019.0
33	57986.0
34	74976.0
35	99020.0
36	137798.0
37	210921.0
38	372913.0
39	442486.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.93406341086952	19.786717010976677	12.366395173069195	23.912824405084603
2	19.194002053741702	21.55382780460115	33.36054647387857	25.89162366777858
3	21.674373513110144	23.851658619655964	28.694534623406277	25.779433243827615
4	14.930510820412406	15.463144265787342	33.95727962139718	35.64906529240307
5	14.470842608124398	37.522626238144746	33.45162545364093	14.554905700089932
6	34.933572298723746	33.90057849183925	15.530624350233118	15.63522485920389
7	28.791608998195002	30.854917818441578	21.450885596382353	18.902587586981063
8	28.57813466677722	31.642483114033688	20.106258809722743	19.673123409466346
9	26.907332878363636	13.844451389464687	20.717980444807285	38.530235287364384
10	18.253235280986306	26.950257355520545	32.13888906605777	22.657618297435373
11	34.62474567406736	22.31511541135426	21.219106175894687	21.84103273868369
12	22.460471850344735	26.493140375158653	28.303877234719714	22.742510539776895
13	30.31660788203104	20.169146676701512	25.665584519124675	23.84866092214278
14	22.960385746267228	21.57449278320269	26.047185034473518	29.417936436056564
15	26.12614566258682	27.65331309356005	23.19331322112165	23.027228022731478
16	24.703706302180667	26.29631283205878	25.26484976432995	23.735131101430603
17	23.523506413159254	26.54569575283665	25.994884779988137	23.93591305401596
18	22.869944574486407	24.80160982734538	28.226383564963932	24.102062033204284
19	23.965443563560754	25.29705906739717	28.38315676682378	22.354340602218294
20	24.70619375330863	24.63979794243145	27.557004088349164	23.097004215910758
21	24.70134641264901	24.981152774145816	27.476640282676495	22.84086053052868
22	24.500181775274736	25.118026366981955	27.685139711838353	22.696652145904952
23	23.900068245454023	25.09748894997672	27.316167794523782	23.686275010045478
24	23.24223309330493	25.860179734289197	27.82392672861908	23.073660443786796
25	23.831057421852577	25.760681689170656	27.17489332661509	23.233367562361675
26	23.58741477290847	26.07103905298271	27.331666528474933	23.009879645633884
27	23.74712189148335	25.25470861742364	27.411009841377155	23.587159649715854
28	23.06721858317335	25.720244663141717	27.64164120749807	23.570895546186865
29	23.07659436050183	26.21142058971726	27.95544273441038	22.756542315370535
30	23.27144469885896	26.15733447288359	27.41777060598136	23.15345022227608
31	22.722547149955037	26.77352076383884	27.561213621027253	22.942718465178874
32	22.590967363365586	26.016378908965663	27.499728931607848	23.8929247960609
33	22.101896203129087	26.39549197318655	27.93197140069011	23.570640422994256
34	23.682703285348914	25.494651980074877	27.499856493204156	23.322788241372052
35	22.955474624809455	25.66552073832652	27.692984750011163	23.686019886852865
36	22.345475071275043	26.558770816458	27.68692557418663	23.408828538080325
37	23.07315019740157	25.357204360055363	28.048562699713624	23.521082742829442
38	22.9575793911485	25.478196534151426	27.87711991427861	23.687104160421462
39	22.85144814302206	25.356630332871987	28.14589219769496	23.646029326410993
40	22.059354410761095	25.238508294692803	28.652247954230898	24.049889340315207
41	21.402475970584295	25.53285667816847	29.02963893690166	24.03502841434558
42	21.865333222779952	25.446242354276823	29.264862520489583	23.423561902453645
43	21.978863043492126	25.090791966170666	28.830387723471972	24.09995726686524
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	634.0
1	598.5
2	563.0
3	1141.0
4	1719.0
5	1719.0
6	2324.0
7	2929.0
8	2804.0
9	2679.0
10	4070.0
11	5461.0
12	5461.0
13	9476.5
14	13492.0
15	17113.5
16	20735.0
17	20101.0
18	19467.0
19	19467.0
20	23011.0
21	26555.0
22	24296.5
23	22038.0
24	24359.0
25	26680.0
26	26680.0
27	29637.0
28	32594.0
29	36134.0
30	39674.0
31	44075.0
32	48476.0
33	48476.0
34	53723.0
35	58970.0
36	63730.0
37	68490.0
38	72837.0
39	77184.0
40	77184.0
41	79343.0
42	81502.0
43	82839.5
44	84177.0
45	86483.0
46	88789.0
47	88789.0
48	94240.5
49	99692.0
50	98683.5
51	97675.0
52	97719.0
53	97763.0
54	97763.0
55	95459.5
56	93156.0
57	91082.5
58	89009.0
59	89857.0
60	90705.0
61	90705.0
62	84230.5
63	77756.0
64	70939.0
65	64122.0
66	55977.0
67	47832.0
68	47832.0
69	42037.0
70	36242.0
71	30721.0
72	25200.0
73	18641.5
74	12083.0
75	12083.0
76	8754.0
77	5425.0
78	4298.0
79	3171.0
80	2661.0
81	2151.0
82	2151.0
83	1664.0
84	1177.0
85	1028.5
86	880.0
87	755.0
88	630.0
89	630.0
90	479.5
91	329.0
92	188.5
93	48.0
94	30.5
95	13.0
96	13.0
97	7.0
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1567870.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.37335015270693
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.81036717406785	52.13595677263692
2	7.85170867657711	9.323644971029198
3	1.7410386901495938	3.1011389933898617
4	0.698114229090421	1.6579752228149052
5	0.3678981701556737	1.0921673438596484
6	0.2327264501300655	0.829064940802132
7	0.1612624513189762	0.67022843920518
8	0.1365262107562808	0.6484814813003938
9	0.09759995816171983	0.5215352841742804
>10	0.702632387991115	8.344848174499937
>50	0.09530216661751272	3.9566286669590327
>100	0.0946863031467228	11.202579453696703
>500	0.007872453447919998	3.150754098574459
>1k	0.0018333110769128763	1.5548236420029993
>5k	4.3136731221479443E-4	1.810172515054433
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	9146	0.5833391799064973	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7406	0.47236059112043727	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6538	0.4169988583237131	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5181	0.3304483152302168	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	2172	0.13853189358811635	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	2113	0.1347688264970948	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	1986	0.12666866513167546	No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT	1835	0.11703776461058632	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	5.740271833761728E-4	0.0
3	0.0	0.0	0.0	8.291503759878051E-4	0.0
4	0.0	0.0	0.0	0.0014669583575168859	0.0
5	0.0	0.0	0.0	0.0017858623482814263	0.0
6	0.0	0.0	0.0	0.0019134239445872427	0.0
7	0.0	0.0	0.0	0.0019134239445872427	0.0
8	0.0	0.0	0.0	0.0019134239445872427	0.0
9	0.0	0.0	0.0	0.0033166015039512203	0.0
10	6.378079815290808E-5	0.0	0.0	0.004974902255926831	0.0
11	6.378079815290808E-5	0.0	0.0	0.005548929439303003	0.0
12	6.378079815290808E-5	0.0	0.0	0.0063142990171379	0.0
13	6.378079815290808E-5	0.0	0.0	0.006888326200514073	0.0
14	6.378079815290808E-5	0.0	0.0	0.007908818970960603	0.0
15	6.378079815290808E-5	0.0	0.0	0.009184434934018765	0.0
16	2.551231926116323E-4	0.0	0.0	0.01199079005274672	0.0
17	2.551231926116323E-4	0.0	0.0	0.016838130712367734	0.0
18	3.1890399076454044E-4	0.0	0.0	0.01824130827173171	0.0
19	3.826847889174485E-4	0.0	0.0	0.020920101794153854	0.0
20	3.826847889174485E-4	0.0	0.0	0.023216210527658543	0.0
21	3.826847889174485E-4	0.0	0.0	0.028765139966961548	0.0
22	3.826847889174485E-4	0.0	0.0	0.036036150956393065	0.0
23	3.826847889174485E-4	0.0	0.0	0.044710339505188566	0.0
24	3.826847889174485E-4	0.0	0.0	0.06435482533628425	0.0
25	5.102463852232646E-4	0.0	0.0	0.07047778195896344	0.0
26	5.102463852232646E-4	0.0	0.0	0.07998112088374675	0.0
27	5.102463852232646E-4	0.0	0.0	0.10109256507235932	0.0
28	5.102463852232646E-4	0.0	0.0	0.14165715269760887	0.0
29	5.740271833761728E-4	0.0	0.0	0.20141976056688374	0.0
30	6.378079815290809E-4	0.0	0.0	0.27719134877253854	0.0
31	6.378079815290809E-4	0.0	0.0	0.5119684667733932	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGGGTA	50	0.0070359968	18.5	25
GGTATCA	4240	0.0	18.194576	1
TCGTTTA	135	1.1532393E-9	17.814816	30
TCTAGCG	160	3.45608E-11	17.34375	28
GTACTAG	90	4.4476183E-5	16.444445	1
CTAGCGG	170	8.54925E-11	16.32353	29
TACACGT	70	0.0025930577	15.857143	5
TGCACCG	85	5.3656986E-4	15.235294	5
CAGTCGG	260	0.0	14.942307	10
CGTTATT	175	2.239176E-9	14.799999	2
TCTTATA	1360	0.0	14.691176	37
GCAGTCG	280	0.0	14.535715	9
TTTCGGA	205	9.276846E-11	14.439025	30
GTTTTCG	205	9.276846E-11	14.439025	28
ACGTTCG	90	8.276384E-4	14.388888	7
TTAACGG	90	8.276384E-4	14.388888	35
CGCAATA	195	6.730261E-10	14.23077	36
ATAAGAC	130	4.4461576E-6	14.230769	3
TTCGGAA	210	1.364242E-10	14.095238	31
GCGTTAT	185	4.9003575E-9	14.0	1
>>END_MODULE