FastQCFastQC Report
Fri 10 Feb 2017
ERR1630722.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630722.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1716
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT170.9906759906759907No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT140.8158508158508158No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT130.7575757575757576No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT50.2913752913752914No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.2913752913752914No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGT40.2331002331002331No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG40.2331002331002331No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC30.17482517482517482No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.17482517482517482No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.17482517482517482No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA30.17482517482517482No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.17482517482517482No Hit
CTACAGGAGGAAACGCGAGCGTGGTGGGACTGGCTCCAGGCAC30.17482517482517482No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG30.17482517482517482No Hit
ATTTAAGGTTCCAAAGACTGGGGTAGGTAAAACTATTGAAGAT20.11655011655011654No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA20.11655011655011654No Hit
CTGGAGGAGAAGTCCCTCATTCCTTGGGATTGGTGACGACAGC20.11655011655011654No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAG20.11655011655011654No Hit
GTGAAAGGCCGAGGAAGGCGAGATTTCCCAGAAGAGGTCGCCA20.11655011655011654No Hit
GGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAATAG20.11655011655011654No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG20.11655011655011654No Hit
CTTAGTAACCTATTGACTATATTTTTTAAGTAGTTGTATTAAT20.11655011655011654No Hit
GTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGC20.11655011655011654No Hit
TCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGG20.11655011655011654No Hit
GACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTC20.11655011655011654No Hit
ATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGAC20.11655011655011654No Hit
GGTATCAACGCAGAGTACATGGGAAACAAAAAAAAACAAAAAA20.11655011655011654No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAA20.11655011655011654No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC20.11655011655011654No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.11655011655011654No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTC20.11655011655011654No Hit
TCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA20.11655011655011654No Hit
GGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGG20.11655011655011654No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG20.11655011655011654No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT20.11655011655011654RNA PCR Primer, Index 26 (100% over 22bp)
GGTATCAACGCAGAGTACATGGGAATGGTATCAACGCAAAAAA20.11655011655011654No Hit
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTC20.11655011655011654RNA PCR Primer, Index 26 (100% over 23bp)
AGGCAGACCCACTCAGTGATCCTGATCAGATGAACGAGGACAA20.11655011655011654No Hit
GCTTGGCCTTCCAAATAAGAACTTACATCACTGGTAAAGGTCC20.11655011655011654No Hit
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC20.11655011655011654No Hit
CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.11655011655011654No Hit
GCGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACG20.11655011655011654No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG20.11655011655011654No Hit
AGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGG20.11655011655011654No Hit
GCCCAGAACTCCTGGACTCAAGCGATCCTCCAGCCTCAGCCTC20.11655011655011654No Hit
CTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGG20.11655011655011654No Hit
GATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGT20.11655011655011654No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.11655011655011654No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGT20.11655011655011654No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)