FastQCFastQC Report
Fri 10 Feb 2017
ERR1630721.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630721.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences646784
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT147192.2757211062735005No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT144422.2328938254502275No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT117861.822246685137542No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85061.3151222046309123No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT25190.3894654165842074No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22630.3498849693251534No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22580.34911191371462497No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA16510.25526296259647735No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGT16320.2523253512764694No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC15870.24536785078171386No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA15530.24011107263012071No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT14890.2302159608153572No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14830.2292882940827231No Hit
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT13960.215837126459529TruSeq Adapter, Index 12 (95% over 22bp)
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC13530.20918884820898478No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA13170.2036228478131803No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC12740.19697456956263604No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT12710.196510736196319No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTC12350.19094473580051455No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA12340.19079012467840886No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG12260.1895532357015634No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC11920.18429645754997032No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG11650.18012195725311694No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTG11190.17300984563625568No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA11160.17254601226993865No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10490.1621870670888581No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCAT10430.16125940035622402No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC10310.15940406689095588No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAG9100.1406961211161686No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8960.1385315654066891No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC8930.13806773204037207No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC8890.13744928755194935No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8810.1362123985751039No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC8740.13513012072036415No Hit
CGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA8650.13373862062141303No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC8460.1308010093014051No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA8350.12910028695824263No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGAC8300.12832723134771423No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA8170.1263172867603404No Hit
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC8150.12600806451612903No Hit
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC8010.12384350880664953No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC7840.12121511973085296No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG7670.1185867306550564No Hit
GTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCAC7640.11812289728873936No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG7500.11595834157925985No Hit
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATG7430.11487606372452008No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT7420.11472145260241441No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG7390.11425761923609737No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC7350.11363917474767465No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA6840.10575400752028498No Hit
ACGCAGAGTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTT6710.10374406293291114No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA6570.10157950722343163No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAAT6540.10111567385711459No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGCC250.00549458829.632
GATATAG250.00549458829.66
GCAGCGT1000.027.7500021
ATACACT556.2546314E-726.909094
GCCGTCT2000.026.82536
AAGTTCT358.865185E-426.428576
GTACTGG509.083064E-625.9000021
CCGTCTT2250.024.66666637
CTATATC400.001930278623.1250024
TATTATA400.001930278623.1250022
CTTTGCG400.001930278623.1250029
AGCGTCA1201.0913936E-1121.5833323
CAGCGTC1251.8189894E-1120.7200012
CGTCACT1001.2871169E-820.351
AAATAAC555.139752E-420.18181833
GCGTTAT555.139752E-420.1818181
TCTTTGC656.896167E-519.9230778
GATACAC759.255502E-619.7333343
GTCACTA1153.0431693E-919.3043462
GAGCTGA1205.160473E-918.56
Produced by FastQC (version 0.11.5)