##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630721.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	646784
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.526709071343756	31.0	31.0	34.0	30.0	34.0
2	31.606752795369086	31.0	31.0	34.0	30.0	34.0
3	31.617153485553136	31.0	31.0	34.0	28.0	34.0
4	35.33938068968929	37.0	35.0	37.0	33.0	37.0
5	35.36241001632693	37.0	35.0	37.0	33.0	37.0
6	35.45275238719572	37.0	35.0	37.0	33.0	37.0
7	35.39891215614487	37.0	35.0	37.0	33.0	37.0
8	35.47505194933703	37.0	35.0	37.0	33.0	37.0
9	36.99181952552939	39.0	37.0	39.0	33.0	39.0
10	36.87893021472393	39.0	37.0	39.0	33.0	39.0
11	37.075815109835744	39.0	37.0	39.0	33.0	39.0
12	37.04139248961013	39.0	37.0	39.0	33.0	39.0
13	37.099503079853555	39.0	37.0	39.0	33.0	39.0
14	38.10754595042549	40.0	37.0	41.0	33.0	41.0
15	38.02274484217297	40.0	37.0	41.0	33.0	41.0
16	37.91584207401544	40.0	37.0	41.0	33.0	41.0
17	38.06467847071047	40.0	37.0	41.0	33.0	41.0
18	38.09179571541659	40.0	37.0	41.0	33.0	41.0
19	38.18181340292895	40.0	38.0	41.0	33.0	41.0
20	38.18735466554522	40.0	38.0	41.0	34.0	41.0
21	38.10950641945379	40.0	37.0	41.0	33.0	41.0
22	37.984617739461704	40.0	37.0	41.0	33.0	41.0
23	37.88269963388086	40.0	37.0	41.0	33.0	41.0
24	37.76484421383336	40.0	37.0	41.0	33.0	41.0
25	37.675423325252325	40.0	36.0	41.0	33.0	41.0
26	37.31181971106273	39.0	36.0	41.0	32.0	41.0
27	37.032018417276866	39.0	35.0	40.0	31.0	41.0
28	36.82123089006531	39.0	35.0	40.0	31.0	41.0
29	36.59488639174748	39.0	35.0	40.0	31.0	41.0
30	36.32757149218286	38.0	35.0	40.0	30.0	41.0
31	36.102270618939244	38.0	35.0	40.0	30.0	41.0
32	35.772570131604986	38.0	35.0	40.0	30.0	41.0
33	35.542748429151	38.0	35.0	40.0	29.0	41.0
34	35.30444785276074	38.0	35.0	40.0	28.0	41.0
35	35.013290372056204	37.0	34.0	40.0	26.0	41.0
36	34.76697475509598	37.0	34.0	40.0	25.0	41.0
37	34.30211167870572	37.0	33.0	40.0	22.0	41.0
38	33.921689466653476	36.0	33.0	40.0	21.0	41.0
39	33.54642971996834	36.0	33.0	40.0	18.0	41.0
40	33.10642501979022	36.0	33.0	40.0	15.0	41.0
41	32.66014774638828	35.0	32.0	40.0	12.0	41.0
42	32.194626026617854	35.0	31.0	40.0	10.0	41.0
43	31.28863577330299	35.0	30.0	39.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	3.0
10	9.0
11	6.0
12	2.0
13	7.0
14	4.0
15	7.0
16	9.0
17	34.0
18	53.0
19	135.0
20	338.0
21	601.0
22	1029.0
23	1765.0
24	2793.0
25	4333.0
26	6426.0
27	8732.0
28	11702.0
29	15351.0
30	18756.0
31	22535.0
32	27553.0
33	33300.0
34	41082.0
35	51387.0
36	71682.0
37	110555.0
38	134299.0
39	82294.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.15886602018603	20.62527830001979	13.444828567187809	18.771027112606372
2	19.313866762319414	23.852167029487433	32.00759449831783	24.82637170987532
3	24.577293192163072	27.50949312289729	28.393250296853356	19.519963388086286
4	14.601319760538296	17.0296420443301	30.462874777359982	37.90616341777162
5	12.373373490995448	42.495794577478726	29.47954804076786	15.651283890757966
6	31.18212571739561	30.977884425094004	17.827435434395408	20.01255442311498
7	25.6206090441322	35.031788046704925	21.24836112210568	18.099241787057192
8	32.52260414605185	28.194884227191768	19.6046902830002	19.677821343756186
9	29.632458440530378	11.801621561448645	23.109415198891746	35.45650479912923
10	21.78733549376608	29.673584999010487	27.370188501880072	21.16889100534336
11	30.357275380961806	24.53539357807243	19.192497031466456	25.914834009499305
12	22.53719943597863	31.34415817336236	26.62805511577281	19.490587274886206
13	29.951266574312292	19.95751286364536	29.892050514545815	20.199170047496537
14	23.12070181080546	23.579278398970906	29.965181575301802	23.33483821492183
15	27.45878067484663	25.492900257272904	28.24961656441718	18.79870250346329
16	21.95725311696022	25.224649960419555	30.87460419552741	21.943492727092817
17	21.399725410647143	28.014298436572332	29.251496635661983	21.33447951711854
18	19.638550118741342	22.312085642192756	34.439627449040174	23.609736790025725
19	20.097435929151	26.34712670690679	35.20371561448644	18.35172174945577
20	21.23475534336038	22.56348332673659	36.7923448941223	19.409416435780724
21	23.027007470809423	24.492411686127053	34.456016227983376	18.02456461508015
22	23.248410597664755	24.884969325153374	33.14321319018405	18.72340688699782
23	20.789784533940235	24.5862606372452	34.0129007520285	20.61105407678607
24	19.110398525628337	27.124202206609933	34.1191185929151	19.646280674846626
25	18.654604937660796	26.490760439342964	34.87965070255294	19.9749839204433
26	19.984724421135958	27.607052740945974	33.54767588561251	18.86054695230556
27	19.791615129625963	27.435279784286564	34.22054348901643	18.552561597071048
28	18.60110948941223	27.716981248763112	34.6308814070849	19.051027854739758
29	18.46056797941817	27.49279512170987	35.59503636453592	18.451600534336038
30	15.921234909954482	29.422341925588757	36.116385068276266	18.540038096180485
31	19.21244186621809	29.36544503265387	32.793328220858896	18.628784880269148
32	16.997328319810016	28.90686844448842	34.212349099544824	19.88345413615674
33	16.34069488422719	29.536135711458538	34.18544676429844	19.93772264001583
34	18.40599025331486	29.15749307342173	33.69672100732238	18.739795665941024
35	16.85786908767069	30.410152384721943	34.24388976845438	18.48808875915298
36	17.51218335642193	30.399638828418762	32.482405254304375	19.605772560854938
37	17.623194142093805	29.240673857114587	33.23938130813378	19.89675069265783
38	17.384938402928952	28.256419453789828	32.61645309717	21.74218904611122
39	18.187679348901643	29.25443424698199	32.98380912329309	19.574077280823275
40	16.798653027904216	29.631685384919848	32.70442682564813	20.865234761527805
41	17.2937178408866	29.53845487829013	32.42566297249159	20.742164308331684
42	16.718873688897684	30.276722986344744	33.18928730457154	19.81511602018603
43	15.82212918068474	28.636762814169803	32.31372452008708	23.227383485058382
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	830.0
1	601.5
2	373.0
3	1116.5
4	1860.0
5	1860.0
6	2970.5
7	4081.0
8	4459.5
9	4838.0
10	6747.5
11	8657.0
12	8657.0
13	15526.5
14	22396.0
15	29991.0
16	37586.0
17	33604.5
18	29623.0
19	29623.0
20	30350.5
21	31078.0
22	22614.0
23	14150.0
24	12860.5
25	11571.0
26	11571.0
27	11794.0
28	12017.0
29	12252.0
30	12487.0
31	13089.5
32	13692.0
33	13692.0
34	14861.0
35	16030.0
36	16598.5
37	17167.0
38	19850.0
39	22533.0
40	22533.0
41	24495.5
42	26458.0
43	28367.0
44	30276.0
45	32814.0
46	35352.0
47	35352.0
48	38756.0
49	42160.0
50	40739.0
51	39318.0
52	37950.5
53	36583.0
54	36583.0
55	35001.0
56	33419.0
57	31547.0
58	29675.0
59	27894.5
60	26114.0
61	26114.0
62	24649.0
63	23184.0
64	21777.5
65	20371.0
66	18985.0
67	17599.0
68	17599.0
69	13626.5
70	9654.0
71	8407.5
72	7161.0
73	5241.0
74	3321.0
75	3321.0
76	2672.0
77	2023.0
78	1550.5
79	1078.0
80	897.5
81	717.0
82	717.0
83	615.0
84	513.0
85	469.0
86	425.0
87	333.0
88	241.0
89	241.0
90	184.0
91	127.0
92	83.0
93	39.0
94	23.0
95	7.0
96	7.0
97	3.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	646784.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.07929411209518
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.96410327679656	24.54148590037419
2	9.460561838215334	5.880551676711164
3	3.3327536681434253	3.1073889436618067
4	1.787463515365328	2.2221241723471437
5	1.130565671357436	1.7568591506578048
6	0.7684580728580883	1.4329880675502238
7	0.5686845648028623	1.2372020392561907
8	0.44225060516592063	1.0995869303362982
9	0.35368973775870544	0.9893184645809359
>10	2.5668815881512304	15.527896154324969
>50	0.32054488610717247	6.923882319176528
>100	0.2640369732676631	16.79154140619756
>500	0.026003641306663793	5.448186188523389
>1k	0.012001680603075597	5.355090666431661
>5k	5.000700251281498E-4	1.3219874973493067
>10k+	0.0015002100753844496	6.363910422520816
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	14719	2.2757211062735005	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	14442	2.2328938254502275	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	11786	1.822246685137542	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8506	1.3151222046309123	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2519	0.3894654165842074	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2263	0.3498849693251534	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2258	0.34911191371462497	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1651	0.25526296259647735	No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGT	1632	0.2523253512764694	No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC	1587	0.24536785078171386	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	1553	0.24011107263012071	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	1489	0.2302159608153572	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1483	0.2292882940827231	No Hit
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT	1396	0.215837126459529	TruSeq Adapter, Index 12 (95% over 22bp)
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	1353	0.20918884820898478	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	1317	0.2036228478131803	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	1274	0.19697456956263604	No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT	1271	0.196510736196319	No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTC	1235	0.19094473580051455	No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA	1234	0.19079012467840886	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	1226	0.1895532357015634	No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC	1192	0.18429645754997032	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	1165	0.18012195725311694	No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTG	1119	0.17300984563625568	No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA	1116	0.17254601226993865	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1049	0.1621870670888581	No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCAT	1043	0.16125940035622402	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	1031	0.15940406689095588	No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAG	910	0.1406961211161686	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	896	0.1385315654066891	No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC	893	0.13806773204037207	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	889	0.13744928755194935	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	881	0.1362123985751039	No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC	874	0.13513012072036415	No Hit
CGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA	865	0.13373862062141303	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	846	0.1308010093014051	No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	835	0.12910028695824263	No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGAC	830	0.12832723134771423	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	817	0.1263172867603404	No Hit
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC	815	0.12600806451612903	No Hit
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC	801	0.12384350880664953	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	784	0.12121511973085296	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG	767	0.1185867306550564	No Hit
GTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCAC	764	0.11812289728873936	No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG	750	0.11595834157925985	No Hit
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATG	743	0.11487606372452008	No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT	742	0.11472145260241441	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	739	0.11425761923609737	No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC	735	0.11363917474767465	No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	684	0.10575400752028498	No Hit
ACGCAGAGTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTT	671	0.10374406293291114	No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA	657	0.10157950722343163	No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAAT	654	0.10111567385711459	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0010822778547397586	0.0
3	0.0	0.0	0.0	0.002009944587373837	0.0
4	0.0	0.0	0.0	0.002783000197902236	0.0
5	0.0	0.0	0.0	0.0032468335642192757	0.0
6	0.0	0.0	0.0	0.0032468335642192757	0.0
7	0.0	0.0	0.0	0.003710666930536315	0.0
8	0.0	0.0	0.0	0.0038652780526419947	0.0
9	0.0	0.0	0.0	0.005566000395804472	0.0
10	0.0	0.0	0.0	0.00788516722738967	0.0
11	0.0	0.0	0.0	0.011905056402137345	0.0
12	0.0	0.0	0.0	0.017625667920047498	0.0
13	0.0	0.0	0.0	0.019481001385315656	0.0
14	0.0	0.0	0.0	0.024428557292697408	0.0
15	0.0	0.0	0.0	0.03231372452008708	0.0
16	0.0	0.0	0.0	0.04715639224223234	0.0
17	0.0	0.0	0.0	0.07390411636651494	0.0
18	0.0	0.0	0.0	0.08565456164654661	0.0
19	0.0	0.0	0.0	0.10358945181080546	0.0
20	0.0	0.0	0.0	0.11503067484662577	0.0
21	0.0	0.0	0.0	0.14425217692459924	0.0
22	0.0	0.0	0.0	0.1988299030279042	0.0
23	0.0	0.0	0.0	0.2908235206807837	0.0
24	0.0	0.0	0.0	0.4310558084306353	0.0
25	0.0	0.0	0.0	0.46491564417177916	0.0
26	0.0	0.0	0.0	0.5227402038393034	0.0
27	0.0	0.0	0.0	0.5748441519889175	0.0
28	0.0	0.0	0.0	0.6538504353849198	0.0
29	0.0	0.0	0.0	0.7647066099346923	0.0
30	0.0	0.0	0.0	0.9681748466257669	0.0
31	0.0	0.0	0.0	1.4921519394419156	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGCC	25	0.005494588	29.6	32
GATATAG	25	0.005494588	29.6	6
GCAGCGT	100	0.0	27.750002	1
ATACACT	55	6.2546314E-7	26.90909	4
GCCGTCT	200	0.0	26.825	36
AAGTTCT	35	8.865185E-4	26.42857	6
GTACTGG	50	9.083064E-6	25.900002	1
CCGTCTT	225	0.0	24.666666	37
CTATATC	40	0.0019302786	23.125002	4
TATTATA	40	0.0019302786	23.125002	2
CTTTGCG	40	0.0019302786	23.125002	9
AGCGTCA	120	1.0913936E-11	21.583332	3
CAGCGTC	125	1.8189894E-11	20.720001	2
CGTCACT	100	1.2871169E-8	20.35	1
AAATAAC	55	5.139752E-4	20.181818	33
GCGTTAT	55	5.139752E-4	20.181818	1
TCTTTGC	65	6.896167E-5	19.923077	8
GATACAC	75	9.255502E-6	19.733334	3
GTCACTA	115	3.0431693E-9	19.304346	2
GAGCTGA	120	5.160473E-9	18.5	6
>>END_MODULE