FastQCFastQC Report
Fri 10 Feb 2017
ERR1630719.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630719.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1797964
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT65130.3622430704952936No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT57770.32130787935687255No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT46420.25818092019639993No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32750.18215047687272937No Hit
GGGATATACAAAGTGGAAATAGACACCAAATCTTACTGGAAGG18350.10205988551494913No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA18220.10133684545408027No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTG1800.018.57
CCTATAC1502.5102054E-1017.2666663
AAGACGG4400.017.2386365
GGTATCA32850.017.1765611
TATACTG1554.0199666E-1016.7096775
CAAGACG4450.016.6292134
AGTACCG904.448201E-516.4444455
ACGGACC4350.016.1609218
GCGCAAG4700.016.1382981
CGCAAGA4700.016.1382982
GACGGAC4550.015.8571427
CTAGCGG2500.015.54000129
TCTAGCG2550.015.23529428
GGACCGT1001.0939088E-414.86
CAGTCGG3650.014.6986310
CGGACCA4700.014.563839
CGCAATA2800.014.53571436
AGTCGGT3600.014.38888911
CCCTATA1551.2111923E-714.322582
TAAGGGT1551.2111923E-714.322584
Produced by FastQC (version 0.11.5)