##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630719.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1797964
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.957673234836736	33.0	31.0	34.0	30.0	34.0
2	32.12732179287238	33.0	31.0	34.0	30.0	34.0
3	32.226143015099304	34.0	31.0	34.0	30.0	34.0
4	35.82245083883771	37.0	35.0	37.0	35.0	37.0
5	35.79687246240748	37.0	35.0	37.0	35.0	37.0
6	35.829855881430326	37.0	35.0	37.0	35.0	37.0
7	35.80961020354134	37.0	35.0	37.0	35.0	37.0
8	35.797960915791414	37.0	35.0	37.0	35.0	37.0
9	37.52336309292066	39.0	37.0	39.0	35.0	39.0
10	37.42051843084734	39.0	37.0	39.0	34.0	39.0
11	37.53365195298682	39.0	37.0	39.0	35.0	39.0
12	37.46381017639953	39.0	37.0	39.0	35.0	39.0
13	37.50693395418373	39.0	37.0	39.0	35.0	39.0
14	38.77673245960431	40.0	38.0	41.0	35.0	41.0
15	38.75534048512651	40.0	38.0	41.0	35.0	41.0
16	38.723459980288816	40.0	38.0	41.0	35.0	41.0
17	38.712255084084	40.0	38.0	41.0	35.0	41.0
18	38.69679203810532	40.0	38.0	41.0	35.0	41.0
19	38.73894861076195	40.0	38.0	41.0	34.0	41.0
20	38.71599320119869	40.0	38.0	41.0	34.0	41.0
21	38.636461575426424	40.0	38.0	41.0	34.0	41.0
22	38.58333036701514	40.0	38.0	41.0	34.0	41.0
23	38.558158005388314	40.0	38.0	41.0	34.0	41.0
24	38.50422088540149	40.0	38.0	41.0	34.0	41.0
25	38.45626720001068	40.0	38.0	41.0	34.0	41.0
26	38.26275720759704	40.0	38.0	41.0	34.0	41.0
27	38.15578565533014	40.0	38.0	41.0	33.0	41.0
28	38.05407505378306	40.0	38.0	41.0	33.0	41.0
29	37.95968273002129	40.0	37.0	41.0	33.0	41.0
30	37.87648807206374	40.0	37.0	41.0	33.0	41.0
31	37.83641830425971	40.0	37.0	41.0	33.0	41.0
32	37.72670976727009	40.0	37.0	41.0	33.0	41.0
33	37.698559592961814	40.0	37.0	41.0	33.0	41.0
34	37.62253248674612	40.0	37.0	41.0	33.0	41.0
35	37.5572074858006	40.0	37.0	41.0	32.0	41.0
36	37.51641078464307	40.0	37.0	41.0	32.0	41.0
37	37.32259544684988	40.0	36.0	41.0	31.0	41.0
38	37.208411291883486	40.0	36.0	41.0	31.0	41.0
39	37.14808917197452	39.0	36.0	41.0	31.0	41.0
40	37.04729794367407	39.0	36.0	41.0	31.0	41.0
41	36.90957549761842	39.0	35.0	41.0	31.0	41.0
42	36.827009328329154	39.0	35.0	41.0	31.0	41.0
43	35.961027584534506	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	5.0
11	5.0
12	7.0
13	8.0
14	3.0
15	5.0
16	11.0
17	18.0
18	59.0
19	136.0
20	290.0
21	538.0
22	967.0
23	1671.0
24	2843.0
25	4473.0
26	6834.0
27	10035.0
28	14309.0
29	19984.0
30	26692.0
31	35220.0
32	45333.0
33	58535.0
34	77281.0
35	103491.0
36	145704.0
37	223536.0
38	407762.0
39	612205.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.6581600076531	18.89982224338196	12.175215966504336	25.266801782460607
2	18.774625075919207	21.198422215350252	34.535229848873506	25.491722859857035
3	20.50230149213221	23.395685341864464	29.25375591502388	26.84825725097944
4	14.789561971207432	15.595807257542422	33.692888177961294	35.921742593288855
5	14.375871819458009	37.09690516606562	34.06436391384922	14.462859100627155
6	34.924559112418265	34.05802340870006	15.876513656558197	15.140903822323473
7	29.098524775801966	30.448106858646778	21.437414764700517	19.01595360085074
8	27.191812516824587	33.23509258249887	20.26358703511305	19.309507865563493
9	26.69697502285919	13.916352051542747	19.821865176388403	39.56480774920966
10	17.15946481687064	26.756820492512638	32.80543993094411	23.278274759672605
11	35.220727445043394	21.87162813048537	21.279068991370238	21.628575433101
12	21.4575486494724	25.643450035707055	28.957309490067658	23.941691824752887
13	29.582405431921888	19.845725498397076	26.171491754006198	24.40037731567484
14	23.24262332282515	21.14992291280582	25.1702481251015	30.43720563926753
15	25.94601449194756	27.261669310397764	22.71124449655277	24.081071701101912
16	25.536885054428232	26.358592274372565	24.19581259691518	23.908710074284024
17	23.745358638993885	26.62839745400909	25.553459357361994	24.07278454963503
18	23.304304201863886	25.04338240365213	27.345152628195002	24.30716076628898
19	24.174232632021553	25.363355439819706	27.07278899911233	23.38962292904641
20	24.704665944368184	24.408775704074166	27.05482423452305	23.8317341170346
21	24.778249175178146	25.186043769508178	26.069876816221015	23.96583023909266
22	24.763232189298563	24.876471386523868	26.114093496866452	24.246202927311113
23	23.952426188733476	25.239548734012473	26.624893490637184	24.183131586616863
24	23.99241586594615	25.766033135257437	26.527783648615877	23.713767350180536
25	24.471513333971092	25.094662629507596	26.351584347628766	24.08223968889255
26	24.076622223804257	26.15686409739016	26.343074722297	23.423438956508587
27	25.038321123226048	24.985594817248842	25.599344591994054	24.376739467531053
28	23.67878333492773	25.37903984729394	26.785964568812275	24.156212248966053
29	23.350189436495945	25.743229564106958	26.858435430297828	24.04814556909927
30	23.20980842775495	26.562378334605143	26.98196404377396	23.24584919386595
31	23.9573206137609	26.095238836817646	26.13656335721961	23.810877192201847
32	23.28261300003782	25.718590583571192	26.41270904200529	24.58608737438569
33	23.234892356020474	25.629100471422117	26.72973429946317	24.406272873094235
34	24.202041865131893	25.407349646600263	26.521109432669398	23.869499055598446
35	24.025286379482573	25.446671902218288	27.19476029553428	23.33328142276486
36	23.198851589909474	25.975102949780975	26.674838873303358	24.15120658700619
37	24.216280192484387	25.014516419683595	26.66510564171474	24.104097746117276
38	23.51615493969846	24.98787517436389	27.28864426651479	24.20732561942286
39	23.76621556382664	25.163963238418567	26.823451415045017	24.246369782709777
40	22.965198413316397	25.03937787408424	27.787263816183195	24.208159896416166
41	22.350892453908976	25.223252523409812	28.36213628304015	24.06371873964106
42	23.4068090351086	24.774800830272465	27.813738206104237	24.004651928514697
43	22.578427599217783	24.51144739271754	28.049671739812364	24.86045326825231
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	277.0
1	261.0
2	245.0
3	685.0
4	1125.0
5	1125.0
6	1568.5
7	2012.0
8	1971.5
9	1931.0
10	2799.0
11	3667.0
12	3667.0
13	6238.0
14	8809.0
15	12249.5
16	15690.0
17	15281.5
18	14873.0
19	14873.0
20	17811.5
21	20750.0
22	20222.0
23	19694.0
24	22026.5
25	24359.0
26	24359.0
27	27162.5
28	29966.0
29	36245.5
30	42525.0
31	48130.5
32	53736.0
33	53736.0
34	58751.0
35	63766.0
36	69916.5
37	76067.0
38	84532.0
39	92997.0
40	92997.0
41	97616.0
42	102235.0
43	100846.5
44	99458.0
45	105800.0
46	112142.0
47	112142.0
48	116115.0
49	120088.0
50	125295.0
51	130502.0
52	138886.5
53	147271.0
54	147271.0
55	135970.0
56	124669.0
57	119007.0
58	113345.0
59	101942.0
60	90539.0
61	90539.0
62	85569.0
63	80599.0
64	71979.0
65	63359.0
66	55137.5
67	46916.0
68	46916.0
69	40155.5
70	33395.0
71	29188.5
72	24982.0
73	19878.5
74	14775.0
75	14775.0
76	11587.5
77	8400.0
78	7007.0
79	5614.0
80	4344.0
81	3074.0
82	3074.0
83	2323.0
84	1572.0
85	1357.0
86	1142.0
87	1000.0
88	858.0
89	858.0
90	662.0
91	466.0
92	259.0
93	52.0
94	35.0
95	18.0
96	18.0
97	11.0
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1797964.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.41158812699654
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.91931121209232	45.0316755140342
2	8.363744773100318	8.76714292494108
3	2.2072132990896494	3.470506630209478
4	0.978150631310992	2.0506571205773345
5	0.5268750976437117	1.3807180306036653
6	0.32622919725911026	1.0258914193047115
7	0.24082866625081228	0.8835549005298041
8	0.17102438959328545	0.7170927893627417
9	0.13643478055773206	0.6435687172310107
>10	0.84971367134439	8.808608036485367
>50	0.12416028806245048	4.5727460981689605
>100	0.14034214662332042	15.024668880455108
>500	0.012777477657524452	4.645680743875047
>1k	0.002981411453422372	2.2916161737730922
>5k	2.1295796095874083E-4	0.6858720204483673
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6513	0.3622430704952936	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5777	0.32130787935687255	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4642	0.25818092019639993	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3275	0.18215047687272937	No Hit
GGGATATACAAAGTGGAAATAGACACCAAATCTTACTGGAAGG	1835	0.10205988551494913	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1822	0.10133684545408027	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.1123693244136144E-4	0.0
2	0.0	0.0	0.0	6.11803128427488E-4	0.0
3	0.0	0.0	0.0	0.0010011323919722531	0.0
4	0.0	0.0	0.0	0.0018910278515031447	0.0
5	0.0	0.0	0.0	0.0020022647839445062	0.0
6	0.0	0.0	0.0	0.0020578832501651867	0.0
7	0.0	0.0	0.0	0.0021135017163858676	0.0
8	0.0	0.0	0.0	0.002224738648827229	0.0
9	0.0	0.0	0.0	0.0037264372367856085	0.0
10	0.0	0.0	0.0	0.006006794351833518	0.0
11	0.0	5.561846622068072E-5	0.0	0.006674215946481687	0.0
12	0.0	5.561846622068072E-5	0.0	0.007341637541129856	0.0
13	0.0	5.561846622068072E-5	0.0	0.007730966804674621	0.0
14	0.0	5.561846622068072E-5	0.0	0.008231533000660747	0.0
15	0.0	5.561846622068072E-5	0.0	0.009399520791295043	0.0
16	0.0	1.1123693244136144E-4	0.0	0.011624259440122272	0.0
17	5.561846622068072E-5	1.1123693244136144E-4	0.0	0.014238327352494265	0.0
18	5.561846622068072E-5	1.1123693244136144E-4	0.0	0.015517552075569922	0.0
19	5.561846622068072E-5	1.1123693244136144E-4	0.0	0.01768667225817647	0.0
20	5.561846622068072E-5	1.1123693244136144E-4	0.0	0.019410844711017575	0.0
21	5.561846622068072E-5	1.1123693244136144E-4	0.0	0.02297042654914114	0.0
22	5.561846622068072E-5	1.1123693244136144E-4	0.0	0.029477787096960785	0.0
23	5.561846622068072E-5	1.1123693244136144E-4	0.0	0.04049024340865557	0.0
24	5.561846622068072E-5	1.6685539866204217E-4	0.0	0.05383867530161894	0.0
25	5.561846622068072E-5	1.6685539866204217E-4	0.0	0.0582881525992734	0.0
26	5.561846622068072E-5	1.6685539866204217E-4	0.0	0.06540731627552053	0.0
27	5.561846622068072E-5	1.6685539866204217E-4	0.0	0.07836641890493914	0.0
28	5.561846622068072E-5	2.224738648827229E-4	0.0	0.10773296906945856	0.0
29	5.561846622068072E-5	2.224738648827229E-4	0.0	0.1480007386132314	0.0
30	5.561846622068072E-5	2.224738648827229E-4	0.0	0.20873610372621476	0.0
31	5.561846622068072E-5	2.224738648827229E-4	0.0	0.40373444629592137	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACCGTG	180	0.0	18.5	7
CCTATAC	150	2.5102054E-10	17.266666	3
AAGACGG	440	0.0	17.238636	5
GGTATCA	3285	0.0	17.176561	1
TATACTG	155	4.0199666E-10	16.709677	5
CAAGACG	445	0.0	16.629213	4
AGTACCG	90	4.448201E-5	16.444445	5
ACGGACC	435	0.0	16.160921	8
GCGCAAG	470	0.0	16.138298	1
CGCAAGA	470	0.0	16.138298	2
GACGGAC	455	0.0	15.857142	7
CTAGCGG	250	0.0	15.540001	29
TCTAGCG	255	0.0	15.235294	28
GGACCGT	100	1.0939088E-4	14.8	6
CAGTCGG	365	0.0	14.69863	10
CGGACCA	470	0.0	14.56383	9
CGCAATA	280	0.0	14.535714	36
AGTCGGT	360	0.0	14.388889	11
CCCTATA	155	1.2111923E-7	14.32258	2
TAAGGGT	155	1.2111923E-7	14.32258	4
>>END_MODULE