FastQCFastQC Report
Fri 10 Feb 2017
ERR1630718.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630718.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1285
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT80.622568093385214No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT60.4669260700389105No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.38910505836575876No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT40.311284046692607No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC30.23346303501945526No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.23346303501945526No Hit
ACTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT20.1556420233463035TruSeq Adapter, Index 11 (95% over 21bp)
TCCATGAGCAGCGCCAGCCCAGCTCTCCCCTCTGCACCCTTGG20.1556420233463035No Hit
CCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGG20.1556420233463035No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAG20.1556420233463035No Hit
AACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAACTTC20.1556420233463035No Hit
GTGAAAGGCCGAGGAAGGCGAGATTTCCCAGAAGAGGTCGCCA20.1556420233463035No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT20.1556420233463035No Hit
CATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGC20.1556420233463035No Hit
CCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTA20.1556420233463035No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAAT20.1556420233463035No Hit
CCGCTACACCATTGCCGCCCTGCTGAGCCCCTACTCCTATTCC20.1556420233463035No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCC20.1556420233463035No Hit
TGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGA20.1556420233463035No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.1556420233463035No Hit
GAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTG20.1556420233463035No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.1556420233463035No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA20.1556420233463035No Hit
GATTAAAGTCCTACGTGATCTGAGTTCAGACCGGAGTAATCCA20.1556420233463035No Hit
GTCGTAGGAGACAGAAGGTGGCATTATAAGTCCAGCGGGCTGA20.1556420233463035No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTG20.1556420233463035No Hit
TAGGTGGCCTGACTGGCATTGTATTAGCAAACTCATCACTAGA20.1556420233463035No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers