##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630718.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1285
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.18443579766537	31.0	30.0	31.0	26.0	34.0
2	30.24591439688716	31.0	30.0	31.0	26.0	34.0
3	30.48326848249027	31.0	30.0	33.0	27.0	34.0
4	34.17821011673152	35.0	35.0	37.0	30.0	37.0
5	34.07859922178989	35.0	33.0	37.0	30.0	37.0
6	34.17665369649806	35.0	33.0	37.0	30.0	37.0
7	34.07704280155642	35.0	33.0	37.0	30.0	37.0
8	33.90817120622568	35.0	33.0	37.0	29.0	37.0
9	35.40544747081712	37.0	34.0	39.0	30.0	39.0
10	35.11828793774319	37.0	34.0	39.0	29.0	39.0
11	35.37509727626459	37.0	34.0	39.0	30.0	39.0
12	35.25136186770428	37.0	34.0	39.0	30.0	39.0
13	35.39066147859922	37.0	35.0	39.0	30.0	39.0
14	36.24357976653697	38.0	34.0	40.0	30.0	41.0
15	36.110505836575875	38.0	34.0	40.0	30.0	41.0
16	36.10894941634241	38.0	34.0	40.0	30.0	41.0
17	36.195330739299614	38.0	34.0	40.0	30.0	41.0
18	36.09338521400778	38.0	34.0	40.0	29.0	41.0
19	36.00622568093385	38.0	34.0	40.0	29.0	41.0
20	36.05058365758755	38.0	34.0	40.0	29.0	41.0
21	36.06848249027237	38.0	34.0	40.0	29.0	41.0
22	35.98287937743191	38.0	34.0	40.0	30.0	41.0
23	35.680155642023344	38.0	34.0	40.0	27.0	41.0
24	35.858365758754864	38.0	34.0	40.0	30.0	41.0
25	35.72996108949416	38.0	34.0	40.0	29.0	41.0
26	35.16809338521401	37.0	34.0	40.0	27.0	41.0
27	35.021011673151754	38.0	33.0	40.0	27.0	40.0
28	34.766536964980546	37.0	33.0	39.0	25.0	40.0
29	34.72529182879377	37.0	33.0	40.0	26.0	40.0
30	34.44824902723735	37.0	32.0	39.0	25.0	40.0
31	34.3307392996109	37.0	33.0	40.0	25.0	40.0
32	34.29182879377432	37.0	33.0	39.0	24.0	40.0
33	34.14007782101167	36.0	32.0	39.0	25.0	40.0
34	33.86147859922179	37.0	32.0	39.0	24.0	40.0
35	33.60233463035019	36.0	31.0	39.0	24.0	40.0
36	33.99377431906615	36.0	32.0	39.0	24.0	40.0
37	33.387548638132294	37.0	31.0	39.0	21.0	40.0
38	33.264591439688715	36.0	31.0	39.0	21.0	40.0
39	33.07704280155642	36.0	31.0	39.0	18.0	40.0
40	32.69182879377432	35.0	30.0	39.0	19.0	40.0
41	32.91439688715953	36.0	30.0	39.0	20.0	40.0
42	32.753307392996106	35.0	30.0	39.0	19.0	40.0
43	31.60389105058366	35.0	29.0	38.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	1.0
21	4.0
22	4.0
23	6.0
24	14.0
25	17.0
26	20.0
27	32.0
28	30.0
29	46.0
30	57.0
31	72.0
32	97.0
33	96.0
34	121.0
35	119.0
36	164.0
37	175.0
38	157.0
39	52.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.07782101167315	20.93385214007782	13.073929961089496	25.914396887159537
2	18.443579766536967	21.71206225680934	33.385214007782096	26.459143968871597
3	21.556420233463037	22.64591439688716	30.35019455252918	25.44747081712062
4	14.319066147859921	14.785992217898833	33.07392996108949	37.82101167315175
5	11.361867704280156	39.61089494163424	34.39688715953307	14.63035019455253
6	34.86381322957198	34.474708171206224	15.252918287937744	15.408560311284047
7	25.136186770428015	30.972762645914397	25.603112840466924	18.28793774319066
8	27.626459143968873	31.75097276264591	20.311284046692606	20.311284046692606
9	26.303501945525294	14.319066147859921	22.17898832684825	37.19844357976654
10	18.28793774319066	28.638132295719842	30.58365758754864	22.490272373540858
11	34.78599221789883	20.93385214007782	22.2568093385214	22.023346303501945
12	21.32295719844358	25.603112840466924	29.416342412451364	23.65758754863813
13	28.638132295719842	20.622568093385212	26.69260700389105	24.04669260700389
14	21.011673151750973	21.011673151750973	27.237354085603112	30.739299610894943
15	24.04669260700389	27.47081712062257	24.82490272373541	23.65758754863813
16	23.501945525291827	25.136186770428015	26.07003891050584	25.291828793774318
17	22.957198443579767	25.36964980544747	28.871595330739297	22.801556420233464
18	22.17898832684825	23.424124513618676	28.871595330739297	25.525291828793772
19	22.101167315175097	24.747081712062258	29.64980544747082	23.501945525291827
20	23.73540856031128	25.525291828793772	29.18287937743191	21.556420233463037
21	22.879377431906615	28.326848249027236	28.715953307392994	20.07782101167315
22	23.65758754863813	26.770428015564203	29.18287937743191	20.389105058365757
23	21.867704280155642	25.214007782101167	28.56031128404669	24.357976653696497
24	21.78988326848249	25.836575875486385	30.739299610894943	21.634241245136188
25	22.64591439688716	26.225680933852143	29.571984435797667	21.556420233463037
26	22.101167315175097	25.36964980544747	30.19455252918288	22.334630350194555
27	23.501945525291827	24.43579766536965	28.249027237354085	23.813229571984433
28	22.957198443579767	25.680933852140075	28.56031128404669	22.801556420233464
29	23.19066147859922	27.47081712062257	26.926070038910506	22.412451361867706
30	21.71206225680934	27.54863813229572	28.638132295719842	22.101167315175097
31	22.490272373540858	27.003891050583658	27.315175097276263	23.19066147859922
32	21.089494163424124	24.747081712062258	30.739299610894943	23.424124513618676
33	21.011673151750973	24.90272373540856	30.428015564202333	23.65758754863813
34	24.669260700389106	23.035019455252918	30.66147859922179	21.634241245136188
35	23.96887159533074	25.44747081712062	30.27237354085603	20.311284046692606
36	23.891050583657588	27.39299610894942	26.303501945525294	22.412451361867706
37	21.867704280155642	26.6147859922179	30.116731517509727	21.40077821011673
38	21.47859922178988	25.44747081712062	29.105058365758758	23.96887159533074
39	22.17898832684825	25.914396887159537	28.326848249027236	23.57976653696498
40	20.233463035019454	28.01556420233463	29.883268482490273	21.867704280155642
41	20.0	26.6147859922179	29.571984435797667	23.813229571984433
42	22.801556420233464	26.381322957198446	28.871595330739297	21.945525291828794
43	22.17898832684825	27.47081712062257	27.003891050583658	23.346303501945524
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	1.0
4	2.0
5	2.0
6	2.0
7	2.0
8	2.5
9	3.0
10	7.0
11	11.0
12	11.0
13	14.0
14	17.0
15	19.0
16	21.0
17	16.0
18	11.0
19	11.0
20	18.5
21	26.0
22	20.0
23	14.0
24	21.0
25	28.0
26	28.0
27	24.5
28	21.0
29	27.0
30	33.0
31	38.5
32	44.0
33	44.0
34	43.0
35	42.0
36	45.0
37	48.0
38	60.0
39	72.0
40	72.0
41	74.0
42	76.0
43	72.5
44	69.0
45	74.0
46	79.0
47	79.0
48	84.0
49	89.0
50	87.0
51	85.0
52	89.0
53	93.0
54	93.0
55	94.0
56	95.0
57	82.0
58	69.0
59	68.0
60	67.0
61	67.0
62	59.0
63	51.0
64	46.5
65	42.0
66	38.0
67	34.0
68	34.0
69	24.5
70	15.0
71	14.5
72	14.0
73	8.0
74	2.0
75	2.0
76	4.0
77	6.0
78	4.0
79	2.0
80	1.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.5
88	1.0
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1285.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.57587548638132
#Duplication Level	Percentage of deduplicated	Percentage of total
1	97.82433521353747	94.47470817120622
2	1.6921837228041903	3.268482490272374
3	0.16116035455278002	0.4669260700389105
4	0.08058017727639001	0.311284046692607
5	0.08058017727639001	0.38910505836575876
6	0.08058017727639001	0.4669260700389105
7	0.0	0.0
8	0.08058017727639001	0.622568093385214
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	8	0.622568093385214	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6	0.4669260700389105	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5	0.38910505836575876	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4	0.311284046692607	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	3	0.23346303501945526	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3	0.23346303501945526	No Hit
ACTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT	2	0.1556420233463035	TruSeq Adapter, Index 11 (95% over 21bp)
TCCATGAGCAGCGCCAGCCCAGCTCTCCCCTCTGCACCCTTGG	2	0.1556420233463035	No Hit
CCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGG	2	0.1556420233463035	No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAG	2	0.1556420233463035	No Hit
AACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAACTTC	2	0.1556420233463035	No Hit
GTGAAAGGCCGAGGAAGGCGAGATTTCCCAGAAGAGGTCGCCA	2	0.1556420233463035	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	2	0.1556420233463035	No Hit
CATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGC	2	0.1556420233463035	No Hit
CCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTA	2	0.1556420233463035	No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAAT	2	0.1556420233463035	No Hit
CCGCTACACCATTGCCGCCCTGCTGAGCCCCTACTCCTATTCC	2	0.1556420233463035	No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCC	2	0.1556420233463035	No Hit
TGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGA	2	0.1556420233463035	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2	0.1556420233463035	No Hit
GAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTG	2	0.1556420233463035	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2	0.1556420233463035	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA	2	0.1556420233463035	No Hit
GATTAAAGTCCTACGTGATCTGAGTTCAGACCGGAGTAATCCA	2	0.1556420233463035	No Hit
GTCGTAGGAGACAGAAGGTGGCATTATAAGTCCAGCGGGCTGA	2	0.1556420233463035	No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTG	2	0.1556420233463035	No Hit
TAGGTGGCCTGACTGGCATTGTATTAGCAAACTCATCACTAGA	2	0.1556420233463035	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.07782101167315175	0.0
19	0.0	0.0	0.0	0.07782101167315175	0.0
20	0.0	0.0	0.0	0.07782101167315175	0.0
21	0.0	0.0	0.0	0.07782101167315175	0.0
22	0.0	0.0	0.0	0.07782101167315175	0.0
23	0.0	0.0	0.0	0.07782101167315175	0.0
24	0.0	0.0	0.0	0.07782101167315175	0.0
25	0.0	0.0	0.0	0.07782101167315175	0.0
26	0.0	0.0	0.0	0.1556420233463035	0.0
27	0.0	0.0	0.0	0.1556420233463035	0.0
28	0.0	0.0	0.0	0.1556420233463035	0.0
29	0.0	0.0	0.0	0.311284046692607	0.0
30	0.0	0.0	0.0	0.311284046692607	0.0
31	0.0	0.0	0.0	0.7003891050583657	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE