FastQCFastQC Report
Fri 10 Feb 2017
ERR1630717.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630717.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2550579
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT80450.31541857750730323No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT73060.2864447641104236No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT62730.24594415620923718No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43860.17196095474792195No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG39760.1558861732963378No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG32610.1278533227161362No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT31420.123187715416774No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC29670.11632652821182954No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA29610.11609128750766003No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA27580.10813231034992447No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCGT450.00382706920.55555510
GGTATCA33750.019.5688881
AGTCGTT852.7252237E-517.4117647
GACGGAC4100.017.1463437
CATAACG650.001580648117.0769232
CTTATAC15200.016.91776337
CGAATTA1107.8167795E-716.81818215
AAGACGG4450.016.2134845
TCTAGCG2500.015.54000128
CGCAAGA4650.015.5161292
TCGCCAT9250.015.213
ACGGACC4550.015.0439568
CTAGTAC750.004106229614.83
ACTGTCG750.004106229614.88
AGTCGGT2500.014.06000111
ACACCGT800.006301399313.8756
CGGACCA4700.013.7765969
GTAACAC1753.571222E-813.7428573
GCGCAAG5400.013.7037041
ATAGACG950.00124580213.6315793
Produced by FastQC (version 0.11.5)