##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630717.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2550579
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.06925251090047	33.0	31.0	34.0	30.0	34.0
2	32.25223253229953	33.0	31.0	34.0	30.0	34.0
3	32.35713969259529	34.0	31.0	34.0	30.0	34.0
4	35.925739606575604	37.0	35.0	37.0	35.0	37.0
5	35.901273005070614	37.0	35.0	37.0	35.0	37.0
6	35.939159304612794	37.0	35.0	37.0	35.0	37.0
7	35.92075171951153	37.0	35.0	37.0	35.0	37.0
8	35.91806095792367	37.0	35.0	37.0	35.0	37.0
9	37.659087995314	39.0	37.0	39.0	35.0	39.0
10	37.578746629686826	39.0	37.0	39.0	35.0	39.0
11	37.673057764531116	39.0	37.0	39.0	35.0	39.0
12	37.610159889185944	39.0	37.0	39.0	35.0	39.0
13	37.64952114794327	39.0	37.0	39.0	35.0	39.0
14	38.95646909976127	40.0	38.0	41.0	36.0	41.0
15	38.94203669049263	40.0	38.0	41.0	36.0	41.0
16	38.9213178654729	40.0	38.0	41.0	36.0	41.0
17	38.91589595930963	40.0	38.0	41.0	36.0	41.0
18	38.90141767810368	40.0	38.0	41.0	35.0	41.0
19	38.94349871146904	40.0	38.0	41.0	35.0	41.0
20	38.925623554494884	40.0	38.0	41.0	35.0	41.0
21	38.86146596517889	40.0	38.0	41.0	35.0	41.0
22	38.81545366757901	40.0	38.0	41.0	35.0	41.0
23	38.79240713579152	40.0	38.0	41.0	35.0	41.0
24	38.75299490821496	40.0	38.0	41.0	35.0	41.0
25	38.71206694636786	40.0	38.0	41.0	35.0	41.0
26	38.550500494201515	40.0	38.0	41.0	34.0	41.0
27	38.450715700239044	40.0	38.0	41.0	34.0	41.0
28	38.376918338934026	40.0	38.0	41.0	34.0	41.0
29	38.29392973124926	40.0	38.0	41.0	34.0	41.0
30	38.23342699834038	40.0	38.0	41.0	34.0	41.0
31	38.19100565008964	40.0	38.0	41.0	34.0	41.0
32	38.09450167981466	40.0	38.0	41.0	33.0	41.0
33	38.0722408519791	40.0	38.0	41.0	33.0	41.0
34	38.013578093444664	40.0	37.0	41.0	33.0	41.0
35	37.976803306229684	40.0	37.0	41.0	33.0	41.0
36	37.93352097700169	40.0	37.0	41.0	33.0	41.0
37	37.7735016245331	40.0	37.0	41.0	33.0	41.0
38	37.6710448882391	40.0	37.0	41.0	33.0	41.0
39	37.62239907095604	40.0	37.0	41.0	33.0	41.0
40	37.54298337749977	40.0	37.0	41.0	33.0	41.0
41	37.44136605845183	40.0	37.0	41.0	32.0	41.0
42	37.367652207596784	40.0	37.0	41.0	32.0	41.0
43	36.56772050581456	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	6.0
10	6.0
11	6.0
12	3.0
13	4.0
14	3.0
15	5.0
16	11.0
17	22.0
18	78.0
19	142.0
20	268.0
21	477.0
22	951.0
23	1687.0
24	2701.0
25	4487.0
26	7062.0
27	10656.0
28	15861.0
29	22515.0
30	31559.0
31	41962.0
32	55291.0
33	73267.0
34	99104.0
35	136011.0
36	193333.0
37	301101.0
38	559620.0
39	992378.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.92746862575125	19.832986941396445	12.588710249711928	24.650834183140375
2	18.034179690180153	21.493315831424944	36.064556322309556	24.407948156085343
3	19.952136358058308	23.9709101345224	30.47998121210909	25.5969722953102
4	14.42209788444114	15.742190302672451	35.03443727875122	34.80127453413519
5	13.707083764117872	37.883711894436516	34.72921246509126	13.67999187635435
6	34.06712750320614	35.21125987471864	16.121202283873583	14.60041033820164
7	27.780006030003385	31.398831402595256	22.11740941958669	18.703753147814673
8	25.96771164508137	34.6125722826072	20.76900970328698	18.650706369024444
9	25.92152605349609	14.542854779248163	20.71419861921548	38.82142054804027
10	16.414469028404923	27.57985539753915	33.84066911865894	22.16500645539699
11	34.334792217767024	22.40546166184227	22.490736417103722	20.76900970328698
12	20.692321233727714	26.572358668365105	29.690082134291863	23.04523796361532
13	28.900379090394768	20.68012792389493	26.377618572096768	24.041874413613538
14	22.208447572100294	22.090356738607195	25.885612639326204	29.815583049966303
15	24.975740802382518	28.689015317698452	23.589702573415682	22.74554130650335
16	24.487812375150895	27.519869017975918	24.705253199371594	23.287065407501593
17	22.99156387628064	27.455530685385554	26.180251621298538	23.372653817035268
18	22.124662674631917	25.989392996648995	28.192186950492413	23.693757378226667
19	23.25922859084153	26.52009602525544	28.06190280716653	22.1587725767365
20	23.71869289286864	25.56874341080986	28.14631501317936	22.566248683142142
21	23.602876052849176	26.668376082450294	26.75729706862638	22.971450796074148
22	23.593662458602537	26.121127790983927	27.0524849455751	23.23272480483843
23	22.8097228119576	26.554754822336417	27.394642549789676	23.24087981591631
24	23.95420804452636	26.59133475183478	26.98649992805555	22.46795727558331
25	23.59813203198176	25.9466576020582	27.31587612067691	23.13933424528313
26	23.384102197971522	26.55761691757048	27.115372627156418	22.94290825730158
27	23.6088746908055	25.985001836837835	26.849864285717086	23.556259186639583
28	22.62125580113378	26.39192904826708	27.735035848722976	23.251779301876162
29	22.653679811525148	26.98763692479237	27.453021451207743	22.905661812474737
30	22.34939596068187	27.639292882125982	27.308662072415714	22.70264908477644
31	23.15666364382362	26.819949509503527	27.175202179583536	22.848184667089317
32	22.27372686750734	26.768039727450123	27.231659948584223	23.72657345645832
33	22.514691762144988	26.305752536972975	27.69492730866207	23.484628392219964
34	22.929460330379886	26.263566037358576	27.45541306503347	23.35156056722807
35	23.138040421410196	26.242904062175686	27.602085644083168	23.01696987233095
36	22.53033526897226	26.721422861240523	27.446630745411138	23.301611124376073
37	23.41052757040656	25.491349219138087	27.373745333902615	23.724377876552737
38	22.53104099108477	25.84668030278615	28.419311850368096	23.202966855760987
39	22.620706906157388	25.64100151377393	28.386887839976726	23.351403740091957
40	22.295408219074965	25.22231226713621	29.199605266098406	23.282674247690426
41	21.505901209098013	25.93234712588789	29.354785717282233	23.206965947731867
42	22.302739887688247	25.19388734871572	29.0812399851171	23.422132778478925
43	21.43693647599231	25.177655740128024	29.067321576787077	24.318086207092584
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	528.0
1	491.0
2	454.0
3	1185.0
4	1916.0
5	1916.0
6	2456.5
7	2997.0
8	2970.0
9	2943.0
10	4320.0
11	5697.0
12	5697.0
13	9210.0
14	12723.0
15	17658.5
16	22594.0
17	23305.0
18	24016.0
19	24016.0
20	27513.0
21	31010.0
22	33207.5
23	35405.0
24	39365.0
25	43325.0
26	43325.0
27	48426.0
28	53527.0
29	67920.5
30	82314.0
31	86157.5
32	90001.0
33	90001.0
34	98346.5
35	106692.0
36	112306.0
37	117920.0
38	133411.0
39	148902.0
40	148902.0
41	157891.5
42	166881.0
43	159191.5
44	151502.0
45	157602.0
46	163702.0
47	163702.0
48	170459.0
49	177216.0
50	179801.5
51	182387.0
52	190348.5
53	198310.0
54	198310.0
55	176539.5
56	154769.0
57	145691.5
58	136614.0
59	124936.5
60	113259.0
61	113259.0
62	104048.5
63	94838.0
64	83816.5
65	72795.0
66	62958.5
67	53122.0
68	53122.0
69	45263.0
70	37404.0
71	32339.5
72	27275.0
73	21348.0
74	15421.0
75	15421.0
76	12046.5
77	8672.0
78	7035.0
79	5398.0
80	4346.0
81	3294.0
82	3294.0
83	2539.5
84	1785.0
85	1549.5
86	1314.0
87	1181.0
88	1048.0
89	1048.0
90	788.0
91	528.0
92	293.5
93	59.0
94	38.5
95	18.0
96	18.0
97	10.0
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2550579.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.75865956271627
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.97436350602601	43.981591522669014
2	9.394663884858975	9.725104194451223
3	2.3421408544807507	3.636782134049956
4	0.9652976376488638	1.9985004721504713
5	0.4794475179186792	1.2407780429071111
6	0.3196120275485321	0.9925614075620379
7	0.22472449065317812	0.8142006884985863
8	0.162291199974813	0.6719979975616842
9	0.12503711772108367	0.5824578247945961
>10	0.7636858382706557	7.767049038685064
>50	0.10828863020527517	3.929222665132057
>100	0.1181859961991074	13.377996463698755
>500	0.016411059640782787	5.7377552783243155
>1k	0.005622307469527379	4.693644026672275
>5k	2.2793138389975862E-4	0.8503582428429345
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8045	0.31541857750730323	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7306	0.2864447641104236	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6273	0.24594415620923718	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4386	0.17196095474792195	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	3976	0.1558861732963378	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	3261	0.1278533227161362	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	3142	0.123187715416774	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	2967	0.11632652821182954	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	2961	0.11609128750766003	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	2758	0.10813231034992447	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	7.841356805650796E-5	0.0
2	0.0	7.841356805650796E-5	0.0	5.488949763955556E-4	0.0
3	0.0	7.841356805650796E-5	0.0	7.841356805650795E-4	0.0
4	0.0	7.841356805650796E-5	0.0	0.0013330306569606352	0.0
5	0.0	2.744474881977778E-4	0.0	0.001450651009045397	0.0
6	3.920678402825398E-5	2.744474881977778E-4	0.0	0.001489857793073651	0.0
7	3.920678402825398E-5	3.136542722260318E-4	0.0	0.001803512065299683	0.0
8	3.920678402825398E-5	3.136542722260318E-4	0.0	0.001803512065299683	0.0
9	3.920678402825398E-5	3.136542722260318E-4	0.0	0.0027836816660060325	0.0
10	3.920678402825398E-5	3.136542722260318E-4	0.0	0.004508780163249207	0.0
11	3.920678402825398E-5	3.528610562542858E-4	0.0	0.005292915843814287	0.0
12	7.841356805650796E-5	3.9206784028253974E-4	0.0	0.005802604036181588	0.0
13	7.841356805650796E-5	4.3127462431079375E-4	0.0	0.006233878660492382	0.0
14	7.841356805650796E-5	4.3127462431079375E-4	0.0	0.00670436006883143	0.0
15	7.841356805650796E-5	4.3127462431079375E-4	0.0	0.0073708753973117476	0.0
16	7.841356805650796E-5	4.3127462431079375E-4	0.0	0.009095973894554923	0.0
17	7.841356805650796E-5	4.3127462431079375E-4	0.0	0.011173933448052384	0.0
18	7.841356805650796E-5	4.3127462431079375E-4	0.0	0.012154103048758733	0.0
19	7.841356805650796E-5	4.3127462431079375E-4	0.0	0.013722374409888893	0.0
20	1.568271361130159E-4	4.3127462431079375E-4	0.0	0.01517302541893429	0.0
21	1.568271361130159E-4	4.3127462431079375E-4	0.0	0.01776067316479905	0.0
22	1.568271361130159E-4	4.704814083390477E-4	0.0	0.021720558351652704	0.0
23	1.568271361130159E-4	4.704814083390477E-4	0.0	0.027131094547551752	0.0
24	1.568271361130159E-4	5.096881923673017E-4	0.0	0.039089163676169214	0.0
25	1.568271361130159E-4	5.096881923673017E-4	0.0	0.04297063529496636	0.0
26	1.568271361130159E-4	5.096881923673017E-4	0.0	0.048773239331147945	0.0
27	1.568271361130159E-4	5.488949763955556E-4	0.0	0.06206433911672605	0.0
28	1.568271361130159E-4	5.881017604238097E-4	0.0	0.09264563065876415	0.0
29	1.568271361130159E-4	5.881017604238097E-4	0.0	0.13773343229125623	0.0
30	1.568271361130159E-4	6.273085444520637E-4	0.0	0.20956026063101751	0.0
31	1.568271361130159E-4	6.273085444520637E-4	0.0	0.4632281532938207	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCGT	45	0.003827069	20.555555	10
GGTATCA	3375	0.0	19.568888	1
AGTCGTT	85	2.7252237E-5	17.411764	7
GACGGAC	410	0.0	17.146343	7
CATAACG	65	0.0015806481	17.076923	2
CTTATAC	1520	0.0	16.917763	37
CGAATTA	110	7.8167795E-7	16.818182	15
AAGACGG	445	0.0	16.213484	5
TCTAGCG	250	0.0	15.540001	28
CGCAAGA	465	0.0	15.516129	2
TCGCCAT	925	0.0	15.2	13
ACGGACC	455	0.0	15.043956	8
CTAGTAC	75	0.0041062296	14.8	3
ACTGTCG	75	0.0041062296	14.8	8
AGTCGGT	250	0.0	14.060001	11
ACACCGT	80	0.0063013993	13.875	6
CGGACCA	470	0.0	13.776596	9
GTAACAC	175	3.571222E-8	13.742857	3
GCGCAAG	540	0.0	13.703704	1
ATAGACG	95	0.001245802	13.631579	3
>>END_MODULE