FastQCFastQC Report
Fri 10 Feb 2017
ERR1630716.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630716.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1963620
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT74140.3775679612144916No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT61020.31075258960491337No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT54140.2757152605901345No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38170.19438587914158545No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA22290.11351483484584594No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT20650.10516291339464866No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC20440.1040934600380929No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31650.021.4518171
ACCGAGT609.239992E-418.58
CGAATAC500.00703661518.49999823
ACCGTTA500.00703661518.4999988
GCTACGT500.00703661518.49999813
CGAATTA752.0682064E-417.26666615
TAATACT3600.016.4444454
GCGCAAG5850.016.4444451
CAAGACG5400.016.4444434
AAGACGG5300.016.405665
ATACCGT3800.016.065796
TACCGTC3350.016.0149257
CGCAAGA5950.015.8571432
ATTACGT1403.4768163E-815.85714217
CTTAACG1201.9371018E-615.41666730
GACGGAC5650.015.38937957
TTAGACT1455.351285E-815.3103464
TCTTATA17200.014.84302337
CTTATAC11100.014.83333337
TCTAGCG3500.014.828
Produced by FastQC (version 0.11.5)