##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630716.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1963620
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.78481732718143	31.0	31.0	34.0	30.0	34.0
2	31.953287295912652	33.0	31.0	34.0	30.0	34.0
3	32.05355109440727	33.0	31.0	34.0	30.0	34.0
4	35.6852853403408	37.0	35.0	37.0	33.0	37.0
5	35.651664782391705	37.0	35.0	37.0	33.0	37.0
6	35.68674438027724	37.0	35.0	37.0	33.0	37.0
7	35.66624601501309	37.0	35.0	37.0	33.0	37.0
8	35.65815840132001	37.0	35.0	37.0	33.0	37.0
9	37.35058310671108	39.0	37.0	39.0	34.0	39.0
10	37.22273046719834	39.0	37.0	39.0	34.0	39.0
11	37.353012293620964	39.0	37.0	39.0	34.0	39.0
12	37.26766380460578	39.0	37.0	39.0	34.0	39.0
13	37.32835884743484	39.0	37.0	39.0	34.0	39.0
14	38.53051914321508	40.0	38.0	41.0	34.0	41.0
15	38.50636783084303	40.0	38.0	41.0	34.0	41.0
16	38.482808282661615	40.0	38.0	41.0	34.0	41.0
17	38.46537721147676	40.0	38.0	41.0	34.0	41.0
18	38.454561473197465	40.0	38.0	41.0	34.0	41.0
19	38.48722512502419	40.0	38.0	41.0	34.0	41.0
20	38.470031370631794	40.0	38.0	41.0	34.0	41.0
21	38.38762998950917	40.0	38.0	41.0	34.0	41.0
22	38.325415304386794	40.0	38.0	41.0	34.0	41.0
23	38.2827069392245	40.0	38.0	41.0	34.0	41.0
24	38.231962905246434	40.0	38.0	41.0	34.0	41.0
25	38.188679072325606	40.0	38.0	41.0	33.0	41.0
26	37.98460343651012	40.0	37.0	41.0	33.0	41.0
27	37.85063250527088	40.0	37.0	41.0	33.0	41.0
28	37.741692893737074	40.0	37.0	41.0	33.0	41.0
29	37.63336236135301	40.0	37.0	41.0	32.0	41.0
30	37.5602942524521	40.0	37.0	41.0	32.0	41.0
31	37.513079414550674	40.0	37.0	41.0	32.0	41.0
32	37.41010022305741	40.0	36.0	41.0	32.0	41.0
33	37.36392479196586	40.0	36.0	41.0	32.0	41.0
34	37.289950703292895	39.0	36.0	41.0	31.0	41.0
35	37.23332569438079	39.0	36.0	41.0	31.0	41.0
36	37.18634002505576	39.0	36.0	41.0	31.0	41.0
37	36.99599973518298	39.0	36.0	41.0	31.0	41.0
38	36.87365070634848	39.0	35.0	41.0	30.0	41.0
39	36.80810085454416	39.0	35.0	41.0	30.0	41.0
40	36.69297012660291	39.0	35.0	41.0	30.0	41.0
41	36.560365040079034	39.0	35.0	41.0	30.0	41.0
42	36.463369185483955	39.0	35.0	41.0	30.0	41.0
43	35.614741650624865	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	1.0
10	5.0
11	5.0
12	3.0
13	3.0
14	4.0
15	7.0
16	12.0
17	51.0
18	95.0
19	212.0
20	415.0
21	786.0
22	1377.0
23	2258.0
24	3901.0
25	5930.0
26	8988.0
27	12932.0
28	18587.0
29	24765.0
30	33792.0
31	43378.0
32	55247.0
33	71093.0
34	92339.0
35	122579.0
36	172199.0
37	263229.0
38	479640.0
39	549785.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.22460557541683	19.918161355048326	12.366649351707562	24.490583717827278
2	19.1803913180758	21.32877033234536	34.43609252299324	25.054745826585588
3	20.766186940446726	23.676271376335546	29.338161151342927	26.219380531874805
4	14.697446552795348	15.599403143174342	34.42203685030709	35.28111345372322
5	14.318198022020553	37.31567207504507	34.04935781872256	14.316772084211813
6	34.77261384585612	34.793340870433184	15.616565323229544	14.817479960481153
7	28.684572371436428	30.825770770311973	21.638962732096843	18.850694126154753
8	27.199610922683615	33.0530347012151	20.294405231154705	19.452949144946576
9	26.591041036453085	13.725313451686173	20.545777696295616	39.13786781556513
10	17.20139334494454	26.636518267281854	33.01713162424502	23.144956763528583
11	35.382100406392276	21.734653344333424	21.58915676149153	21.29408948778277
12	21.576374247563173	26.12501400474634	28.927236430673958	23.37137531701653
13	29.59926054939347	20.07292653364704	25.871502632892312	24.456310284067182
14	22.884977745184916	21.767042503131968	25.47152707754046	29.876452674142655
15	25.726719018954785	27.96696916918752	22.90361678939917	23.40269502245852
16	25.246585388211567	26.56756398895917	24.435532333139815	23.75031828968945
17	23.33812041026268	26.57326774019413	25.920442855542312	24.168168994000876
18	23.251596541082286	24.805257636406232	27.731740357095568	24.21140546541592
19	23.57798352023304	25.649260040129963	27.334005561157454	23.438750878479542
20	24.391328261068843	24.715983744308982	27.32178323708253	23.570904757539644
21	25.037532720180078	25.40679968629368	26.411780283354215	23.14388731017203
22	24.740683024210387	25.099764720261557	26.722685652010064	23.43686660351799
23	23.748281235676963	25.170756052596733	27.13788818610525	23.943074525621046
24	23.744359906702925	25.891058351412187	26.781862071072815	23.58271967081207
25	24.11912691865025	25.351035332701848	26.754769252706733	23.77506849594117
26	23.778989824915207	26.283700512319086	26.717185606176347	23.220124056589363
27	24.426467442784244	25.277599535551687	26.46224829651358	23.833684725150487
28	23.43681567716768	25.488230920442856	27.44130738126521	23.63364602112425
29	22.895264867947972	25.912803902995492	27.461474215988837	23.730457013067703
30	22.929894786160254	26.567360283557917	27.336704657724	23.16604027255783
31	23.70305863659975	26.390696774324972	26.377659628645056	23.528584960430226
32	23.028029863211824	25.841761644309997	26.645939642089612	24.484268850388567
33	22.86878316578564	25.639431254519714	27.44303887717583	24.048746702518816
34	23.789684358480763	25.3504242164981	27.130656644360922	23.72923478066021
35	23.665016652916552	25.320886933317038	27.73780059278272	23.276295820983695
36	22.87993603650401	26.119870443364807	27.04092441511087	23.95926910502032
37	23.792536234098247	25.218270337438	27.108809240077004	23.880384188386756
38	22.952353306647925	25.106690703904018	27.890019453865822	24.05093653558224
39	23.26030494698567	24.997199050732828	27.666045365192858	24.07645063708864
40	22.684022366853057	25.095588759535957	28.43615363461362	23.78423523899736
41	22.14262433668429	25.209765636935867	28.853546001772234	23.79406402460761
42	22.896945437508275	25.12023711308705	28.56331673134313	23.41950071806154
43	22.095313757244273	24.429319318401728	28.662979598904066	24.812387325449933
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	377.0
1	361.5
2	346.0
3	846.0
4	1346.0
5	1346.0
6	1881.0
7	2416.0
8	2270.5
9	2125.0
10	3192.0
11	4259.0
12	4259.0
13	7378.5
14	10498.0
15	13808.5
16	17119.0
17	17252.0
18	17385.0
19	17385.0
20	20800.5
21	24216.0
22	23820.5
23	23425.0
24	26592.0
25	29759.0
26	29759.0
27	33925.5
28	38092.0
29	44936.5
30	51781.0
31	57467.0
32	63153.0
33	63153.0
34	69516.0
35	75879.0
36	83193.0
37	90507.0
38	97383.5
39	104260.0
40	104260.0
41	109174.5
42	114089.0
43	114423.0
44	114757.0
45	117406.5
46	120056.0
47	120056.0
48	124098.5
49	128141.0
50	127881.0
51	127621.0
52	133502.0
53	139383.0
54	139383.0
55	135369.5
56	131356.0
57	125567.5
58	119779.0
59	111996.5
60	104214.0
61	104214.0
62	96782.5
63	89351.0
64	79531.5
65	69712.0
66	60460.0
67	51208.0
68	51208.0
69	43352.5
70	35497.0
71	30664.5
72	25832.0
73	19991.5
74	14151.0
75	14151.0
76	10889.0
77	7627.0
78	6384.0
79	5141.0
80	4209.0
81	3277.0
82	3277.0
83	2539.0
84	1801.0
85	1656.5
86	1512.0
87	1429.0
88	1346.0
89	1346.0
90	1024.5
91	703.0
92	394.0
93	85.0
94	56.0
95	27.0
96	27.0
97	18.0
98	9.0
99	5.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1963620.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.85881944293049
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.60224372880184	50.10703583257404
2	8.506703423615686	9.843756348830771
3	2.017908398973885	3.502613931258089
4	0.8098831911182311	1.8743554129909639
5	0.44229847760187513	1.2795433877724967
6	0.28038768045563656	0.973374010650286
7	0.1762063595160738	0.7136564357955627
8	0.12584693942558806	0.5825084276536409
9	0.10789181811910846	0.5618243901530693
>10	0.7114985768174386	8.271572768769717
>50	0.11010677815035741	4.468439570776593
>100	0.09807899567870038	11.570992867434809
>500	0.007767867676248664	2.9785591571128727
>1k	0.0029129503785932493	2.3049614766745727
>5k	2.648136707812044E-4	0.9668059815525838
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7414	0.3775679612144916	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6102	0.31075258960491337	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5414	0.2757152605901345	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3817	0.19438587914158545	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	2229	0.11351483484584594	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	2065	0.10516291339464866	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	2044	0.1040934600380929	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	5.092635031217853E-5	0.0	5.601898534339638E-4	0.0
3	0.0	5.092635031217853E-5	0.0	0.0012731587578044632	0.0
4	5.092635031217853E-5	5.092635031217853E-5	0.0	0.0017824222609262484	0.0
5	5.092635031217853E-5	5.092635031217853E-5	0.0	0.001935201311862784	0.0
6	5.092635031217853E-5	5.092635031217853E-5	0.0	0.001935201311862784	0.0
7	5.092635031217853E-5	5.092635031217853E-5	0.0	0.002138906713111498	0.0
8	5.092635031217853E-5	5.092635031217853E-5	0.0	0.0023426121143602123	0.0
9	5.092635031217853E-5	5.092635031217853E-5	0.0	0.00346299182122814	0.0
10	5.092635031217853E-5	5.092635031217853E-5	0.0	0.005245414082154389	0.0
11	5.092635031217853E-5	5.092635031217853E-5	0.0	0.005805603935588352	0.0
12	1.0185270062435706E-4	5.092635031217853E-5	0.0	0.006365793789022316	0.0
13	1.0185270062435706E-4	5.092635031217853E-5	0.0	0.006773204591519744	0.0
14	1.0185270062435706E-4	5.092635031217853E-5	0.0	0.007180615394017173	0.0
15	1.0185270062435706E-4	5.092635031217853E-5	0.0	0.00799543699901203	0.0
16	1.0185270062435706E-4	5.092635031217853E-5	0.0	0.009879711960562634	0.0
17	1.0185270062435706E-4	5.092635031217853E-5	0.0	0.012375103125859382	0.0
18	1.0185270062435706E-4	5.092635031217853E-5	0.0	0.013393630132102952	0.0
19	1.0185270062435706E-4	5.092635031217853E-5	0.0	0.015074199692404843	0.0
20	1.0185270062435706E-4	5.092635031217853E-5	0.0	0.01670384290239456	0.0
21	1.0185270062435706E-4	5.092635031217853E-5	0.0	0.019861276621749625	0.0
22	1.0185270062435706E-4	5.092635031217853E-5	0.0	0.025412248805777086	0.0
23	1.0185270062435706E-4	1.5277905093653558E-4	0.0	0.0332039804035404	0.0
24	1.0185270062435706E-4	1.5277905093653558E-4	0.0	0.044713335574092745	0.0
25	1.0185270062435706E-4	1.5277905093653558E-4	0.0	0.04858373819781832	0.0
26	1.0185270062435706E-4	1.5277905093653558E-4	0.0	0.0559680589930842	0.0
27	1.0185270062435706E-4	1.5277905093653558E-4	0.0	0.06788482496613397	0.0
28	1.0185270062435706E-4	2.0370540124871412E-4	0.0	0.09355170552347196	0.0
29	1.0185270062435706E-4	2.5463175156089263E-4	0.0	0.13505668102789745	0.0
30	1.0185270062435706E-4	2.5463175156089263E-4	0.0	0.19840906081624754	0.0
31	1.0185270062435706E-4	2.5463175156089263E-4	0.0	0.4196331265723511	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3165	0.0	21.451817	1
ACCGAGT	60	9.239992E-4	18.5	8
CGAATAC	50	0.007036615	18.499998	23
ACCGTTA	50	0.007036615	18.499998	8
GCTACGT	50	0.007036615	18.499998	13
CGAATTA	75	2.0682064E-4	17.266666	15
TAATACT	360	0.0	16.444445	4
GCGCAAG	585	0.0	16.444445	1
CAAGACG	540	0.0	16.444443	4
AAGACGG	530	0.0	16.40566	5
ATACCGT	380	0.0	16.06579	6
TACCGTC	335	0.0	16.014925	7
CGCAAGA	595	0.0	15.857143	2
ATTACGT	140	3.4768163E-8	15.857142	17
CTTAACG	120	1.9371018E-6	15.416667	30
GACGGAC	565	0.0	15.3893795	7
TTAGACT	145	5.351285E-8	15.310346	4
TCTTATA	1720	0.0	14.843023	37
CTTATAC	1110	0.0	14.833333	37
TCTAGCG	350	0.0	14.8	28
>>END_MODULE