FastQCFastQC Report
Fri 10 Feb 2017
ERR1630715.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630715.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2012799
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT85680.42567588716011884No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT71870.35706496277074856No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT71180.353636900654263No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51720.25695561255743865No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34800.020.9454041
CGAATTA806.9654743E-720.812515
CCGTCTA500.007036674318.49999827
AAGACGG3650.017.7397255
TACACCG752.068239E-417.2666665
GACGGAC3800.016.065797
GCGCAAG4150.016.0481931
ACCGCTT700.002593401615.8571428
CACTACG700.002593401615.8571427
TACACGG700.002593401615.8571425
CGCAAGA4000.015.7249992
GTAAACG2550.015.23529427
CGAAAGC4150.015.15662719
AGCGTCA1101.45286685E-515.1363643
TCTTATA19350.015.10594437
ACGGACC4200.014.5357148
GCGTTAT2550.014.5098041
CAAGACG4750.014.4105274
ACGAACG1601.7852472E-713.87515
TCTAGCG2009.840733E-1013.87499928
Produced by FastQC (version 0.11.5)