FastQCFastQC Report
Fri 10 Feb 2017
ERR1630714.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630714.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1313727
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT130970.996934675164627No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT127980.9741749998287315No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT102550.7806035805003627No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56890.433042785906052No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT42820.3259429089909852No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA39590.30135637008297766No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC31530.24000420178621587No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT29880.22744451472794577No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC29000.22074601496353508No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGT28870.2197564638619744No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG26870.20453260076104093No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC26750.2036191689749849No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG23750.1807833743235847No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC23190.17652069265532339No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC22570.171801295094034No Hit
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT22480.171116221254492TruSeq Adapter, Index 8 (95% over 22bp)
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA22310.16982219289091266No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG22310.16982219289091266No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC22290.1696699542599033No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG22180.16883264178935198No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGG21830.16616846574668862No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC21240.16167742613191324No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC20510.15612071610007253No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCG20420.15543564226053053No Hit
GTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGC19560.14888938112712916No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAG19480.14828042660309182No Hit
GCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAA19470.14820430728758716No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA19250.14652968234648447No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAA19060.1450834153518958No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC18890.14378938698831645No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCC18610.14165804615418576No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGG18220.13868939284950374No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC17960.1367102906463824No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGG17320.1318386544540837No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG17100.130164029512981No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC16860.128337165940869No Hit
GAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATG16790.12780433073233632No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG16560.12605358647572898No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT16260.12377000701058896No Hit
AGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTA16010.12186702412297229No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGG15980.12163866617645827No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC15450.11760434245471091No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAG15330.11669091066865489No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGG15330.11669091066865489No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAG15160.11539688230507555No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGT14850.11303718352443089No Hit
GACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTC14690.1118192744763562No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT14530.11060136542828153No Hit
AATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTT14510.11044912679727219No Hit
CTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCG14420.10976405295773019No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14410.10968793364222551No Hit
AAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGG14400.10961181432672085No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCT14320.10900285980268351No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTT14250.10847002459415084No Hit
GTCTGTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCC14180.10793718938561818No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAAT14180.10793718938561818No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG14000.10656704170653415No Hit
GTGCCAGGTGGGGAGTTTGACTGGGGCGGTACACCTGTCAAAC13720.10443570087240349No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC13630.10375062703286146No Hit
ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGC13380.10184764414524479No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA13330.10146704756772144No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13320.1013909282522168No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTACG250.005496625329.621
AAGTCGT250.005496625329.617
TTAGGTT400.001931333723.1254
GCCGTCT3150.022.3174636
AAGACGG8050.021.1428595
GCAGTCG4600.020.9130429
GTAAGAT806.9616726E-720.81253
AGCGTCA450.003826094820.5555553
ATAATAC450.003826094820.5555553
GCCGCGT450.003826094820.55555521
ACGTGGT450.003826094820.55555511
TATTACG450.003826094820.55555525
TATCGTG555.1436306E-420.18181837
ACATGTT1303.274181E-1119.92307729
CAGTCGG4750.019.86315710
GGTATCA53300.019.784241
TATAACT759.267429E-619.7333324
AGTCGGT4750.019.47368411
GGCAGTC5350.019.0186928
CAAGACG9100.018.9065934
Produced by FastQC (version 0.11.5)