##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630714.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1313727
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.76123958782913	31.0	31.0	34.0	30.0	34.0
2	31.889078172253445	33.0	31.0	34.0	30.0	34.0
3	31.975098327125803	33.0	31.0	34.0	30.0	34.0
4	35.617728036342406	37.0	35.0	37.0	33.0	37.0
5	35.61759482754027	37.0	35.0	37.0	33.0	37.0
6	35.65899231727749	37.0	35.0	37.0	33.0	37.0
7	35.62712496584146	37.0	35.0	37.0	33.0	37.0
8	35.639836891530734	37.0	35.0	37.0	33.0	37.0
9	37.29331208082044	39.0	37.0	39.0	34.0	39.0
10	37.18889312619745	39.0	37.0	39.0	34.0	39.0
11	37.32293847960801	39.0	37.0	39.0	34.0	39.0
12	37.23593181840672	39.0	37.0	39.0	34.0	39.0
13	37.28473876231515	39.0	37.0	39.0	34.0	39.0
14	38.417646893152074	40.0	38.0	41.0	34.0	41.0
15	38.367035160273026	40.0	38.0	41.0	34.0	41.0
16	38.31017859874997	40.0	38.0	41.0	34.0	41.0
17	38.33384637751983	40.0	38.0	41.0	34.0	41.0
18	38.34667019860291	40.0	38.0	41.0	34.0	41.0
19	38.35828448376261	40.0	38.0	41.0	34.0	41.0
20	38.35317154934016	40.0	38.0	41.0	34.0	41.0
21	38.267439886673564	40.0	38.0	41.0	34.0	41.0
22	38.15234672043735	40.0	38.0	41.0	33.0	41.0
23	38.07166633554765	40.0	37.0	41.0	33.0	41.0
24	37.97879011392778	40.0	37.0	41.0	33.0	41.0
25	37.91273301073967	40.0	37.0	41.0	33.0	41.0
26	37.65720427455628	40.0	37.0	41.0	32.0	41.0
27	37.485373292929204	40.0	36.0	41.0	32.0	41.0
28	37.27238383621559	39.0	36.0	41.0	31.0	41.0
29	37.12966164203065	39.0	36.0	41.0	31.0	41.0
30	36.9904447423247	39.0	35.0	41.0	31.0	41.0
31	36.89115622956672	39.0	35.0	41.0	31.0	41.0
32	36.72386880988211	39.0	35.0	41.0	30.0	41.0
33	36.55176455991237	39.0	35.0	40.0	30.0	41.0
34	36.431261593923246	39.0	35.0	40.0	30.0	41.0
35	36.27711617406052	38.0	35.0	40.0	30.0	41.0
36	36.18934755851102	38.0	35.0	40.0	30.0	41.0
37	35.93542341749846	38.0	35.0	40.0	29.0	41.0
38	35.723303243367916	38.0	35.0	40.0	28.0	41.0
39	35.56910834595011	38.0	35.0	40.0	27.0	41.0
40	35.325888864276976	38.0	34.0	40.0	26.0	41.0
41	35.07575394279024	38.0	34.0	40.0	26.0	41.0
42	34.85667570202942	38.0	34.0	40.0	24.0	41.0
43	33.896939013965614	37.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	6.0
11	6.0
12	1.0
13	6.0
14	7.0
15	11.0
16	13.0
17	39.0
18	99.0
19	218.0
20	410.0
21	760.0
22	1432.0
23	2428.0
24	3751.0
25	5833.0
26	8329.0
27	12034.0
28	16597.0
29	22069.0
30	28049.0
31	35051.0
32	43124.0
33	54978.0
34	71481.0
35	92859.0
36	129937.0
37	199273.0
38	297502.0
39	287419.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.338546745252245	19.151086945765748	11.769720801962661	22.74064550701934
2	21.01182361327734	21.388157509132416	31.442453416881893	26.157565460708348
3	23.0661317001173	23.58914751694987	27.381944650600925	25.962776132331904
4	14.27511195248328	15.156649745342829	32.36737921957911	38.20085908259478
5	15.310410762662258	37.36697198124116	30.871786908543402	16.450830347553183
6	35.11764620807824	33.087163466991235	15.377700237568384	16.41749008736214
7	29.184602280382453	30.722669169469764	19.549419323801672	20.543309226346114
8	31.120620950928163	28.482706072113917	19.12025862298636	21.27641435397156
9	27.77099047214528	11.586121012965403	20.27925132086042	40.3636371940289
10	20.92733117306716	24.516661376374238	28.109569187510036	26.44643826304856
11	38.22323816135316	20.62582256435317	18.39537438143541	22.755564892858256
12	23.542790853807528	26.562900815770707	25.174180023703556	24.720128306718216
13	31.0250150906543	17.547861922606447	25.683189886483266	25.74393310025599
14	24.81010133764473	20.806073103468226	23.722584677029552	30.661240881857495
15	28.28030481218701	25.086947288135207	22.375196673281437	24.25755122639635
16	27.780200909321344	23.98610974730671	23.480753611671222	24.752935731700727
17	24.32308995704587	25.670858557371506	23.821159190608093	26.184892294974528
18	25.20980386335974	20.74076273076522	27.28253282455183	26.76690058132321
19	24.4198376070523	24.053779818790357	26.96991079577416	24.55647177838318
20	25.602884008625843	21.275729280132023	26.999368970874464	26.12201774036767
21	27.762769586070775	22.485721919394212	26.312848864337873	23.438659630197144
22	27.471537084949915	22.052450775541647	26.347026436999464	24.128985702508967
23	24.685950734056618	22.078559700759747	26.989930175751887	26.245559389431744
24	24.306724304212366	24.096482754788475	27.03697191273377	24.559821028265386
25	24.70467608567077	23.830750224361683	26.66901114158421	24.79556254838334
26	24.72888202800125	25.714855521733206	26.172789323809287	23.38347312645626
27	26.305617529364927	23.12763610704507	26.40579054856907	24.16095581502093
28	23.178636048433198	23.214107649458374	28.592089528494125	25.015166773614304
29	22.74300520579999	24.13568420227338	28.532183627191948	24.58912696473468
30	22.257135614933695	25.892974720014127	28.49008964571787	23.359800019334305
31	23.989002281295885	25.692704800921344	25.619021303512827	24.699271614269936
32	23.836002457131507	24.28571537313308	26.004489517228464	25.87379265250695
33	22.36728026446895	23.849551695291336	27.696241304319695	26.08692673592002
34	24.984795166727945	23.844908417045552	27.478996777869373	23.691299638357133
35	24.46368233278299	23.411256676615462	28.378498729188028	23.74656226141352
36	21.476684273064343	26.093853593630946	26.74794687176255	25.68151526154216
37	24.391673460315573	24.59772844738671	26.976609295538573	24.033988796759147
38	22.826660333539618	22.83389166851256	28.27170332953498	26.067744668412846
39	24.213249784772636	23.83744872412609	27.066353968518577	24.8829475225827
40	22.72055000772611	23.972560509146877	28.74592666512906	24.560962817997954
41	21.25685168988686	25.046451812286723	29.080547176087574	24.61614932173884
42	23.001354162622828	24.462007707841888	28.838335514151726	23.69830261538356
43	22.13770440890687	22.468975669983184	28.533553774871034	26.859766146238904
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	600.0
1	470.0
2	340.0
3	790.5
4	1241.0
5	1241.0
6	1964.5
7	2688.0
8	2736.0
9	2784.0
10	4089.0
11	5394.0
12	5394.0
13	9645.0
14	13896.0
15	20570.5
16	27245.0
17	24668.5
18	22092.0
19	22092.0
20	23335.0
21	24578.0
22	17710.5
23	10843.0
24	9717.0
25	8591.0
26	8591.0
27	8956.0
28	9321.0
29	10189.5
30	11058.0
31	12425.0
32	13792.0
33	13792.0
34	17438.5
35	21085.0
36	25620.0
37	30155.0
38	36816.0
39	43477.0
40	43477.0
41	54952.5
42	66428.0
43	67415.5
44	68403.0
45	72015.5
46	75628.0
47	75628.0
48	84806.0
49	93984.0
50	92035.5
51	90087.0
52	95131.0
53	100175.0
54	100175.0
55	102501.0
56	104827.0
57	104130.5
58	103434.0
59	99081.0
60	94728.0
61	94728.0
62	89873.0
63	85018.0
64	72530.0
65	60042.0
66	52706.0
67	45370.0
68	45370.0
69	37220.5
70	29071.0
71	24339.5
72	19608.0
73	14350.0
74	9092.0
75	9092.0
76	6886.0
77	4680.0
78	4527.0
79	4374.0
80	3582.5
81	2791.0
82	2791.0
83	2431.0
84	2071.0
85	2066.5
86	2062.0
87	1758.0
88	1454.0
89	1454.0
90	1259.0
91	1064.0
92	593.0
93	122.0
94	73.5
95	25.0
96	25.0
97	16.0
98	7.0
99	4.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1313727.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.341276348283316
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.56982575874504	22.83451697161629
2	9.565488502648009	5.42196306119748
3	3.0640506420100593	2.6051731797102606
4	1.4587697093572365	1.653735818455935
5	0.881252378116845	1.2487908590395684
6	0.5884213531546594	1.0005967307392207
7	0.4306542366267232	0.8543703511557871
8	0.3396183656271152	0.7700174362552318
9	0.2704599495966035	0.6898662155394106
>10	2.0857327325475907	12.12242779218023
>50	0.3337707807209418	6.717610946176625
>100	0.3367359508973488	19.76197698702242
>500	0.04933761330174278	9.616039104959178
>1k	0.024803608873007296	11.50602414465886
>5k	2.6960444427181845E-4	0.43469273866389685
>10k+	8.088133328154553E-4	2.762197662629614
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	13097	0.996934675164627	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	12798	0.9741749998287315	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	10255	0.7806035805003627	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5689	0.433042785906052	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	4282	0.3259429089909852	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	3959	0.30135637008297766	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	3153	0.24000420178621587	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	2988	0.22744451472794577	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	2900	0.22074601496353508	No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGT	2887	0.2197564638619744	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG	2687	0.20453260076104093	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC	2675	0.2036191689749849	No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG	2375	0.1807833743235847	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	2319	0.17652069265532339	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC	2257	0.171801295094034	No Hit
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT	2248	0.171116221254492	TruSeq Adapter, Index 8 (95% over 22bp)
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA	2231	0.16982219289091266	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	2231	0.16982219289091266	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC	2229	0.1696699542599033	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG	2218	0.16883264178935198	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGG	2183	0.16616846574668862	No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC	2124	0.16167742613191324	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	2051	0.15612071610007253	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCG	2042	0.15543564226053053	No Hit
GTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGC	1956	0.14888938112712916	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAG	1948	0.14828042660309182	No Hit
GCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAA	1947	0.14820430728758716	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA	1925	0.14652968234648447	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAA	1906	0.1450834153518958	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	1889	0.14378938698831645	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCC	1861	0.14165804615418576	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGG	1822	0.13868939284950374	No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC	1796	0.1367102906463824	No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGG	1732	0.1318386544540837	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG	1710	0.130164029512981	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	1686	0.128337165940869	No Hit
GAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATG	1679	0.12780433073233632	No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG	1656	0.12605358647572898	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1626	0.12377000701058896	No Hit
AGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTA	1601	0.12186702412297229	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGG	1598	0.12163866617645827	No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC	1545	0.11760434245471091	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAG	1533	0.11669091066865489	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGG	1533	0.11669091066865489	No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAG	1516	0.11539688230507555	No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGT	1485	0.11303718352443089	No Hit
GACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTC	1469	0.1118192744763562	No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT	1453	0.11060136542828153	No Hit
AATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTT	1451	0.11044912679727219	No Hit
CTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCG	1442	0.10976405295773019	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1441	0.10968793364222551	No Hit
AAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGG	1440	0.10961181432672085	No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCT	1432	0.10900285980268351	No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTT	1425	0.10847002459415084	No Hit
GTCTGTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCC	1418	0.10793718938561818	No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAAT	1418	0.10793718938561818	No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG	1400	0.10656704170653415	No Hit
GTGCCAGGTGGGGAGTTTGACTGGGGCGGTACACCTGTCAAAC	1372	0.10443570087240349	No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC	1363	0.10375062703286146	No Hit
ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGC	1338	0.10184764414524479	No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA	1333	0.10146704756772144	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1332	0.1013909282522168	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.522386310093345E-4	0.0
2	0.0	0.0	0.0	0.0016746249411026797	0.0
3	0.0	0.0	0.0	0.002664176042663354	0.0
4	0.0	0.0	0.0	0.003958204406242697	0.0
5	0.0	0.0	0.0	0.004414920299270701	0.0
6	0.0	0.0	0.0	0.004643278245784702	0.0
7	0.0	0.0	0.0	0.005099994138812706	0.0
8	0.0	7.611931550466725E-5	0.0	0.005480590716336042	0.0
9	0.0	7.611931550466725E-5	0.0	0.008068647443494729	0.0
10	0.0	7.611931550466725E-5	0.0	0.011646255272214091	0.0
11	0.0	7.611931550466725E-5	0.0	0.013396999528821438	0.0
12	0.0	7.611931550466725E-5	0.0	0.015147743785428784	0.0
13	0.0	7.611931550466725E-5	0.0	0.0172029653040548	0.0
14	0.0	7.611931550466725E-5	0.0	0.018953709560662148	0.0
15	0.0	7.611931550466725E-5	0.0	0.021237289025802165	0.0
16	0.0	7.611931550466725E-5	0.0	0.02473877753901686	0.0
17	0.0	7.611931550466725E-5	0.0	0.029229817153792227	0.0
18	0.0	7.611931550466725E-5	0.0	0.032274589773978915	0.0
19	0.0	7.611931550466725E-5	0.0	0.03638503281123095	0.0
20	0.0	7.611931550466725E-5	0.0	0.039810402008940975	0.0
21	0.0	7.611931550466725E-5	0.0	0.04711785629738903	0.0
22	0.0	7.611931550466725E-5	0.0	0.06158052624327581	0.0
23	0.0	1.522386310093345E-4	0.0	0.07847901428531194	0.0
24	0.0	1.522386310093345E-4	0.0	0.10618644512901082	0.0
25	0.0	1.522386310093345E-4	0.0	0.11539688230507555	0.0
26	0.0	1.522386310093345E-4	0.0	0.13069686472151368	0.0
27	0.0	1.522386310093345E-4	0.0	0.15330430142639986	0.0
28	0.0	1.522386310093345E-4	0.0	0.20932811763783496	0.0
29	0.0	1.522386310093345E-4	0.0	0.2926026487999409	0.0
30	0.0	1.522386310093345E-4	0.0	0.430302490547884	0.0
31	0.0	1.522386310093345E-4	0.0	0.8210229370333411	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTACG	25	0.0054966253	29.6	21
AAGTCGT	25	0.0054966253	29.6	17
TTAGGTT	40	0.0019313337	23.125	4
GCCGTCT	315	0.0	22.31746	36
AAGACGG	805	0.0	21.142859	5
GCAGTCG	460	0.0	20.913042	9
GTAAGAT	80	6.9616726E-7	20.8125	3
AGCGTCA	45	0.0038260948	20.555555	3
ATAATAC	45	0.0038260948	20.555555	3
GCCGCGT	45	0.0038260948	20.555555	21
ACGTGGT	45	0.0038260948	20.555555	11
TATTACG	45	0.0038260948	20.555555	25
TATCGTG	55	5.1436306E-4	20.181818	37
ACATGTT	130	3.274181E-11	19.923077	29
CAGTCGG	475	0.0	19.863157	10
GGTATCA	5330	0.0	19.78424	1
TATAACT	75	9.267429E-6	19.733332	4
AGTCGGT	475	0.0	19.473684	11
GGCAGTC	535	0.0	19.018692	8
CAAGACG	910	0.0	18.906593	4
>>END_MODULE