FastQCFastQC Report
Fri 10 Feb 2017
ERR1630713.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630713.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2333380
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT83570.3581499798575457No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT72670.31143662841028896No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT65840.28216578525572344No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44750.1917818786481413No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT23850.10221224146945633No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35650.019.3562411
AAGACGG4550.016.670335
GCAGTCG2400.016.18759
ACCGTTC803.3844338E-416.18758
TCGCCAT3250.015.93846113
TTCGGGC4200.015.85714235
CGGTGAT2350.015.74468114
CGAACGA4150.015.60240916
TAGGACG1201.937482E-615.4166664
TACCGTC4200.015.4166667
TCGGTGA2650.015.35849113
ACGCTTC4350.015.31034631
TAACGAA4350.014.88505813
ATAACGA4350.014.88505812
GTCTAAT1752.240995E-914.81
TTAACGG1252.961724E-614.79999935
CGGACCA5250.014.7999999
CGCGGTT5900.014.73728810
GTAAACG4650.014.72042927
ATACCGT4400.014.7159096
Produced by FastQC (version 0.11.5)