##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630713.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2333380
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.973395246380786	33.0	31.0	34.0	30.0	34.0
2	32.146996631495945	33.0	31.0	34.0	30.0	34.0
3	32.250113569157186	34.0	31.0	34.0	30.0	34.0
4	35.83661255346322	37.0	35.0	37.0	35.0	37.0
5	35.81386700837412	37.0	35.0	37.0	35.0	37.0
6	35.847781330087685	37.0	35.0	37.0	35.0	37.0
7	35.82638961506484	37.0	35.0	37.0	35.0	37.0
8	35.82283725754056	37.0	35.0	37.0	35.0	37.0
9	37.54656378301006	39.0	37.0	39.0	35.0	39.0
10	37.449295442662574	39.0	37.0	39.0	34.0	39.0
11	37.558379689549064	39.0	37.0	39.0	35.0	39.0
12	37.481099949429584	39.0	37.0	39.0	35.0	39.0
13	37.53253306362444	39.0	37.0	39.0	35.0	39.0
14	38.79806718151351	40.0	38.0	41.0	35.0	41.0
15	38.77275668772339	40.0	38.0	41.0	35.0	41.0
16	38.75137482964627	40.0	38.0	41.0	35.0	41.0
17	38.74865988394518	40.0	38.0	41.0	35.0	41.0
18	38.73679254986329	40.0	38.0	41.0	35.0	41.0
19	38.76510384078033	40.0	38.0	41.0	35.0	41.0
20	38.74757047716189	40.0	38.0	41.0	34.0	41.0
21	38.68159836803264	40.0	38.0	41.0	34.0	41.0
22	38.622372695403236	40.0	38.0	41.0	34.0	41.0
23	38.589355784312886	40.0	38.0	41.0	34.0	41.0
24	38.54219501324259	40.0	38.0	41.0	34.0	41.0
25	38.497701617396224	40.0	38.0	41.0	34.0	41.0
26	38.32007816986518	40.0	38.0	41.0	34.0	41.0
27	38.21297302625376	40.0	38.0	41.0	34.0	41.0
28	38.11106892147871	40.0	38.0	41.0	33.0	41.0
29	38.01600682272069	40.0	37.0	41.0	33.0	41.0
30	37.94712048616171	40.0	37.0	41.0	33.0	41.0
31	37.9024055233181	40.0	37.0	41.0	33.0	41.0
32	37.806539869202616	40.0	37.0	41.0	33.0	41.0
33	37.76145848511601	40.0	37.0	41.0	33.0	41.0
34	37.695713085738284	40.0	37.0	41.0	33.0	41.0
35	37.644144117117655	40.0	37.0	41.0	33.0	41.0
36	37.59245386520841	40.0	37.0	41.0	33.0	41.0
37	37.42067301511113	40.0	36.0	41.0	32.0	41.0
38	37.315103840780324	40.0	36.0	41.0	31.0	41.0
39	37.25114212001474	40.0	36.0	41.0	31.0	41.0
40	37.14736090992466	40.0	36.0	41.0	31.0	41.0
41	37.029046276217336	39.0	36.0	41.0	31.0	41.0
42	36.93555314607993	39.0	36.0	41.0	31.0	41.0
43	36.11661238203807	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	6.0
10	3.0
11	3.0
12	6.0
13	1.0
14	3.0
15	3.0
16	14.0
17	33.0
18	99.0
19	161.0
20	287.0
21	618.0
22	1184.0
23	2127.0
24	3334.0
25	5400.0
26	8293.0
27	12183.0
28	18073.0
29	24972.0
30	33666.0
31	44624.0
32	57668.0
33	74806.0
34	98709.0
35	133059.0
36	186859.0
37	286731.0
38	520655.0
39	819800.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.02059673092252	20.053527500878555	12.393137851528683	24.53273791667024
2	18.32864771276003	21.66269531752222	35.07624133231621	24.932415637401537
3	20.11704051633253	24.285628573142823	30.12291182747774	25.47441908304691
4	14.213330019113904	15.73824237800958	35.42419151616968	34.62423608670684
5	13.92237869528324	37.72025988051668	34.321070721442716	14.036290702757373
6	33.68855480033257	35.62878742425151	16.06137877242455	14.621279002991368
7	27.899484867445505	31.5192124728934	22.206798721168433	18.37450393849266
8	26.919104475053356	33.4592308153837	20.628144579965543	18.993520129597407
9	26.468084924015805	14.146259931944218	20.843068852908655	38.54258629113132
10	17.021745279380127	26.96410357507136	33.189579065561546	22.82457207998697
11	34.9168159493953	22.249140731471083	22.067258654826905	20.766784664306716
12	21.21047579048419	26.65450976694752	29.266043250563563	22.868971192004732
13	29.120803298219748	20.286108563543014	26.32635918710197	24.266728951135264
14	22.396480641815735	22.02993082995483	25.73687097686618	29.836717551363257
15	25.139925772913113	28.342961712194324	23.433774181659224	23.083338333233336
16	24.723576957032286	27.104757904841904	24.818932192784715	23.352732945341092
17	23.073652812658032	27.067387223684097	26.175290779898685	23.683669183759182
18	22.716574239943775	25.240209481524655	28.193393274991642	23.84982300353993
19	23.32594776675895	26.146791349887287	27.818400774841646	22.708860108512116
20	23.96742065158697	25.182310639501498	27.75313065167268	23.097138057238855
21	24.524209515809684	26.094720962723606	26.729208272977395	22.651861248489315
22	24.21950132425923	25.62047330481962	27.061344487395967	23.098680883525187
23	23.21554997471479	25.709185816283675	27.5142497150057	23.561014493995835
24	23.709682949198157	26.205504461339345	27.04055918881622	23.044253400646273
25	23.73282534349313	25.66791521312431	27.279440125483205	23.319819317899356
26	23.41174605079327	26.550711842906	27.10102940798327	22.93651269831746
27	23.87575105640744	25.777327310596643	26.80026399472011	23.546657638275807
28	22.863614156288303	26.07886413700297	27.85217152799801	23.205350178710713
29	22.659661092492435	26.496584354027203	27.740959466524956	23.102795086955403
30	22.499978571857135	27.044501967103514	27.646032793629843	22.80948666740951
31	23.190436191276177	26.71845134525881	26.908390403620498	23.18272205984452
32	22.681517798215463	26.183347761616194	27.00978837566106	24.12534606450728
33	22.381395229238272	26.043636270131742	27.856714294285545	23.718254206344444
34	23.254592051016125	25.982437494107263	27.37500964266429	23.387960812212327
35	23.329804832474778	25.94043833409046	27.860614216287104	22.869142617147656
36	22.592633861608483	26.569740033770756	27.33511044064833	23.502515663972435
37	23.463345018813907	25.43546271931704	27.41585168296634	23.685340578902707
38	22.551963246449358	25.5068612913456	28.407374709648664	23.533800752556377
39	22.977611876333903	25.456548011896906	28.01502541377744	23.550814697991754
40	22.508635541574883	25.3358647112772	28.844037404966187	23.311462342181727
41	21.780721528426575	25.777455879453843	29.117074801361113	23.324747790758472
42	22.591476741893736	25.411120349021594	28.897307768130354	23.100095140954323
43	21.83574899930573	24.950886696551784	28.79668120923296	24.41668309490953
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	702.0
1	617.0
2	532.0
3	1216.0
4	1900.0
5	1900.0
6	2505.5
7	3111.0
8	2920.5
9	2730.0
10	4139.5
11	5549.0
12	5549.0
13	9071.0
14	12593.0
15	16993.5
16	21394.0
17	21862.5
18	22331.0
19	22331.0
20	26556.5
21	30782.0
22	32000.5
23	33219.0
24	37000.5
25	40782.0
26	40782.0
27	45158.5
28	49535.0
29	60029.0
30	70523.0
31	75783.5
32	81044.0
33	81044.0
34	87882.0
35	94720.0
36	102897.5
37	111075.0
38	120306.0
39	129537.0
40	129537.0
41	136594.0
42	143651.0
43	141022.0
44	138393.0
45	141800.5
46	145208.0
47	145208.0
48	150394.5
49	155581.0
50	154212.5
51	152844.0
52	157232.5
53	161621.0
54	161621.0
55	153045.0
56	144469.0
57	137094.5
58	129720.0
59	121416.5
60	113113.0
61	113113.0
62	105065.5
63	97018.0
64	86402.5
65	75787.0
66	65919.5
67	56052.0
68	56052.0
69	48063.0
70	40074.0
71	34487.5
72	28901.0
73	22269.0
74	15637.0
75	15637.0
76	11935.5
77	8234.0
78	6717.0
79	5200.0
80	4399.5
81	3599.0
82	3599.0
83	2827.0
84	2055.0
85	1902.0
86	1749.0
87	1625.5
88	1502.0
89	1502.0
90	1146.5
91	791.0
92	438.0
93	85.0
94	57.5
95	30.0
96	30.0
97	17.5
98	5.0
99	3.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2333380.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.66329853392152
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.55044151631039	45.05368441282248
2	9.029872248339	9.510857158749
3	2.244208191891472	3.5456221794555853
4	0.8832357682187674	1.8605643575016984
5	0.4654769040576269	1.2256774579516172
6	0.28709827362208434	0.9071725255399988
7	0.2079701843026251	0.7666677131458678
8	0.15596189971543853	0.6570774467705336
9	0.12235651701703561	0.5799328004915095
>10	0.7856162740187326	8.232336065942805
>50	0.12038757989446434	4.505631151006181
>100	0.12966039993044715	14.334233034127896
>500	0.013061192760843306	4.554869868099611
>1k	0.004408152556784612	3.3109442833154894
>5k	2.448973642658118E-4	0.9547295450798585
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8357	0.3581499798575457	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7267	0.31143662841028896	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6584	0.28216578525572344	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4475	0.1917818786481413	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	2385	0.10221224146945633	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.285628573142823E-5	0.0
2	0.0	0.0	0.0	5.142754287771387E-4	0.0
3	0.0	0.0	0.0	7.285568574342799E-4	0.0
4	0.0	0.0	0.0	0.0014571137148685599	0.0
5	0.0	4.285628573142823E-5	0.0	0.0017999640007199855	0.0
6	0.0	4.285628573142823E-5	0.0	0.0017999640007199855	0.0
7	0.0	4.285628573142823E-5	0.0	0.0019713891436456987	0.0
8	0.0	4.285628573142823E-5	0.0	0.0020999580008399833	0.0
9	0.0	4.285628573142823E-5	0.0	0.003557071715708543	0.0
10	0.0	4.285628573142823E-5	0.0	0.0049284728591142465	0.0
11	0.0	4.285628573142823E-5	0.0	0.0058713111452056675	0.0
12	0.0	4.285628573142823E-5	0.0	0.0067712931455656605	0.0
13	0.0	8.571257146285646E-5	0.0	0.007328424860074227	0.0
14	0.0	8.571257146285646E-5	0.0	0.008014125431777078	0.0
15	0.0	8.571257146285646E-5	0.0	0.009085532575062784	0.0
16	0.0	8.571257146285646E-5	0.0	0.011056921718708482	0.0
17	0.0	8.571257146285646E-5	0.0	0.013242592291011322	0.0
18	0.0	8.571257146285646E-5	0.0	0.0142711431485656	0.0
19	0.0	8.571257146285646E-5	0.0	0.01551397543477702	0.0
20	0.0	8.571257146285646E-5	0.0	0.01714251429257129	0.0
21	0.0	8.571257146285646E-5	0.0	0.020528160865354122	0.0
22	0.0	8.571257146285646E-5	0.0	0.0251994960100798	0.0
23	0.0	8.571257146285646E-5	0.0	0.03265648972734831	0.0
24	0.0	8.571257146285646E-5	0.0	0.04422768687483393	0.0
25	0.0	8.571257146285646E-5	0.0	0.049027590876753895	0.0
26	0.0	8.571257146285646E-5	0.0	0.0551131834506167	0.0
27	0.0	8.571257146285646E-5	0.0	0.06822720688443373	0.0
28	0.0	1.2856885719428468E-4	0.0	0.09814089432497064	0.0
29	0.0	1.714251429257129E-4	0.0	0.13962577891299316	0.0
30	0.0	1.714251429257129E-4	0.0	0.20725299779718692	0.0
31	0.0	1.714251429257129E-4	0.0	0.4321199290299908	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3565	0.0	19.356241	1
AAGACGG	455	0.0	16.67033	5
GCAGTCG	240	0.0	16.1875	9
ACCGTTC	80	3.3844338E-4	16.1875	8
TCGCCAT	325	0.0	15.938461	13
TTCGGGC	420	0.0	15.857142	35
CGGTGAT	235	0.0	15.744681	14
CGAACGA	415	0.0	15.602409	16
TAGGACG	120	1.937482E-6	15.416666	4
TACCGTC	420	0.0	15.416666	7
TCGGTGA	265	0.0	15.358491	13
ACGCTTC	435	0.0	15.310346	31
TAACGAA	435	0.0	14.885058	13
ATAACGA	435	0.0	14.885058	12
GTCTAAT	175	2.240995E-9	14.8	1
TTAACGG	125	2.961724E-6	14.799999	35
CGGACCA	525	0.0	14.799999	9
CGCGGTT	590	0.0	14.737288	10
GTAAACG	465	0.0	14.720429	27
ATACCGT	440	0.0	14.715909	6
>>END_MODULE