FastQCFastQC Report
Fri 10 Feb 2017
ERR1630712.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630712.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1434554
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT76680.5345215307335939No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT73860.5148638531557543No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT60110.41901524794465733No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45750.31891445006601354No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTTA902.152974E-618.525
CAGTCGG2900.018.510
AGTCGGT2900.018.511
GCTACGA852.7238788E-517.4117642
GGTATCA38050.017.2115631
CGAACTA1301.3938916E-817.07692324
GCAGTCG3150.017.0317469
CGCTTAT1005.879836E-616.6526
GTCGGTG3150.016.44444512
CTAGGAC1708.54925E-1116.3235283
TCGTTCT2100.015.85714230
CGTCAAT2150.015.48837224
GACGGAC2400.015.4166667
GACACGC3800.015.09210630
TCTAGCG1353.973837E-715.07407328
CTTATAC14750.015.05084737
CTAGGTA4150.014.7108434
TCGGTGA3550.014.59154913
CGGACCA2550.014.5098049
TAGACAG1956.730261E-1014.230775
Produced by FastQC (version 0.11.5)