##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630712.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1434554
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.94500032762796	33.0	31.0	34.0	30.0	34.0
2	32.10439342123057	33.0	31.0	34.0	30.0	34.0
3	32.21647355205869	34.0	31.0	34.0	30.0	34.0
4	35.804061053121735	37.0	35.0	37.0	35.0	37.0
5	35.78251846915487	37.0	35.0	37.0	35.0	37.0
6	35.82495674613852	37.0	35.0	37.0	35.0	37.0
7	35.79881133787923	37.0	35.0	37.0	35.0	37.0
8	35.80188964653823	37.0	35.0	37.0	35.0	37.0
9	37.514212779721085	39.0	37.0	39.0	35.0	39.0
10	37.42375748839012	39.0	37.0	39.0	34.0	39.0
11	37.53681841185483	39.0	37.0	39.0	35.0	39.0
12	37.467595503550235	39.0	37.0	39.0	35.0	39.0
13	37.52390499068003	39.0	37.0	39.0	35.0	39.0
14	38.769397317912045	40.0	38.0	41.0	35.0	41.0
15	38.74341572363257	40.0	38.0	41.0	35.0	41.0
16	38.71169088092885	40.0	38.0	41.0	35.0	41.0
17	38.72633585072434	40.0	38.0	41.0	35.0	41.0
18	38.72573914958935	40.0	38.0	41.0	35.0	41.0
19	38.75797704373624	40.0	38.0	41.0	35.0	41.0
20	38.748497442410674	40.0	38.0	41.0	35.0	41.0
21	38.673966961160055	40.0	38.0	41.0	34.0	41.0
22	38.60716571143366	40.0	38.0	41.0	34.0	41.0
23	38.582138420721705	40.0	38.0	41.0	34.0	41.0
24	38.52951300543584	40.0	38.0	41.0	34.0	41.0
25	38.487647031760396	40.0	38.0	41.0	34.0	41.0
26	38.28139128955759	40.0	38.0	41.0	34.0	41.0
27	38.16360137018195	40.0	38.0	41.0	33.0	41.0
28	38.065877617712545	40.0	37.0	41.0	33.0	41.0
29	37.955055020584794	40.0	37.0	41.0	33.0	41.0
30	37.86616188724858	40.0	37.0	41.0	33.0	41.0
31	37.803070501354426	40.0	37.0	41.0	33.0	41.0
32	37.693529138673064	40.0	37.0	41.0	33.0	41.0
33	37.63828060846786	40.0	37.0	41.0	33.0	41.0
34	37.54275684289333	40.0	36.0	41.0	33.0	41.0
35	37.47057412965981	40.0	36.0	41.0	32.0	41.0
36	37.40647058249463	40.0	36.0	41.0	32.0	41.0
37	37.195828111036604	40.0	36.0	41.0	31.0	41.0
38	37.07720866555041	40.0	36.0	41.0	31.0	41.0
39	36.983065119890924	39.0	36.0	41.0	31.0	41.0
40	36.850358369221375	39.0	35.0	41.0	31.0	41.0
41	36.70780395858225	39.0	35.0	41.0	30.0	41.0
42	36.59224748597822	39.0	35.0	41.0	30.0	41.0
43	35.7729489444106	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	7.0
10	4.0
11	5.0
12	4.0
13	1.0
14	0.0
15	3.0
16	3.0
17	21.0
18	50.0
19	98.0
20	197.0
21	427.0
22	771.0
23	1298.0
24	2250.0
25	3615.0
26	5567.0
27	8403.0
28	12073.0
29	16568.0
30	22253.0
31	29206.0
32	37356.0
33	47939.0
34	62402.0
35	83607.0
36	116331.0
37	180216.0
38	314449.0
39	489430.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.86485276957159	19.943968648095506	12.461921963202501	23.729256619130407
2	18.533216595541194	21.52188066813797	34.837238612140084	25.107664124180758
3	20.747633062261862	24.381863631484073	29.29879251669857	25.571710789555503
4	14.174788819382192	15.763505591284819	34.80433640002398	35.25736918930901
5	13.9481678626249	37.96978015466828	33.99049460668612	14.091557376020699
6	34.090107448029144	34.738113727332674	16.162305497039497	15.009473327598682
7	28.26585823886727	31.2607960383506	22.023987943291086	18.449357779491045
8	27.680589228429188	32.81354344277037	20.408782102311935	19.09708522648851
9	26.729492232429035	13.814049523405881	21.114436960895162	38.34202128326992
10	17.49324180198166	27.3353948335162	32.80587555435348	22.36548781014866
11	34.191044742825994	22.51940324309855	22.005027346478418	21.284524667597037
12	21.76181586751004	26.638314068344588	29.382511916595682	22.21735814754969
13	29.64572961352448	20.55217161570774	26.185560111365625	23.616538659402156
14	22.406894407599854	21.916776921607692	26.7342323816322	28.942096289160258
15	25.35931028040771	28.172728248640343	23.784953372267616	22.68300809868433
16	24.011783453254463	27.023451191101906	25.783623342167672	23.181142013475966
17	22.570708387415184	27.26903274467186	26.83035981914937	23.32989904876359
18	22.36005058018032	25.579448386048902	28.778003477038855	23.28249755673192
19	23.048069295404705	26.187511937508102	28.861583460782935	21.902835306304258
20	23.7942245464444	25.349899690077894	28.339330551516362	22.516545211961347
21	24.172112029243934	25.74646893738402	27.8398721832709	22.241546850101145
22	23.919071711486637	25.741310539721752	27.96506788869572	22.37454986009589
23	23.221363573626366	25.78996677713073	27.882812358405467	23.10585729083743
24	22.76596070973975	26.464462125510785	28.054015394331618	22.715561770417843
25	23.06486894184534	26.221947727307583	27.83485320176166	22.878330129085416
26	22.80186036914609	26.84395289407021	27.86949811579069	22.484688620993005
27	23.082714209433732	26.122474302117592	27.85520796010467	22.939603528344
28	22.452553197718593	26.449962845595216	28.26815860539234	22.829325351293853
29	22.280234832568173	26.918052579407952	28.547897116455704	22.253815471568167
30	22.406824699523337	26.9738887486982	28.073463947679905	22.545822604098557
31	22.28692680791382	27.227556439144152	27.900239377534756	22.58527737540727
32	21.96543315901667	26.66340897589076	28.058825251611303	23.312332613481264
33	21.763837401729038	26.783864532112418	28.444241206674686	23.00805685948385
34	22.96713821856828	26.042867678734993	28.101695718669355	22.88829838402737
35	22.304214410890076	26.14896337119411	28.410851038022965	23.135971179892845
36	21.86526265306151	26.7244732509198	28.229470622925312	23.18079347309338
37	22.310418429700103	25.65013237563731	28.67678734993594	23.362661844726652
38	21.959159432130125	25.597642194019883	28.991170775028337	23.452027598821655
39	21.934901021502153	25.49928409805417	29.250484819672177	23.3153300607715
40	21.2466034739717	25.454949761389255	29.751197933294947	23.5472488313441
41	20.71424289361014	25.652781282544957	30.09541641513669	23.537559408708212
42	21.054209182784337	25.681431301993513	30.092349259769936	23.172010255452218
43	20.849546270129952	25.312954409523797	29.827110028622133	24.010389291724117
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	850.0
1	687.5
2	525.0
3	1195.0
4	1865.0
5	1865.0
6	2468.0
7	3071.0
8	2884.0
9	2697.0
10	4142.5
11	5588.0
12	5588.0
13	9038.0
14	12488.0
15	16338.5
16	20189.0
17	19626.5
18	19064.0
19	19064.0
20	21910.0
21	24756.0
22	23230.0
23	21704.0
24	24062.0
25	26420.0
26	26420.0
27	29450.0
28	32480.0
29	36219.5
30	39959.0
31	44116.5
32	48274.0
33	48274.0
34	53308.5
35	58343.0
36	63127.5
37	67912.0
38	72004.0
39	76096.0
40	76096.0
41	78727.0
42	81358.0
43	83259.5
44	85161.0
45	86097.0
46	87033.0
47	87033.0
48	90023.5
49	93014.0
50	92026.5
51	91039.0
52	89838.0
53	88637.0
54	88637.0
55	85955.0
56	83273.0
57	80081.5
58	76890.0
59	74673.5
60	72457.0
61	72457.0
62	66881.0
63	61305.0
64	55207.0
65	49109.0
66	42575.5
67	36042.0
68	36042.0
69	31327.0
70	26612.0
71	22619.5
72	18627.0
73	14106.0
74	9585.0
75	9585.0
76	7176.5
77	4768.0
78	3819.0
79	2870.0
80	2358.5
81	1847.0
82	1847.0
83	1425.5
84	1004.0
85	858.5
86	713.0
87	633.5
88	554.0
89	554.0
90	436.0
91	318.0
92	182.0
93	46.0
94	25.5
95	5.0
96	5.0
97	5.0
98	5.0
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1434554.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.45409099028188
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.33597058791169	56.93614685988124
2	7.42052900104007	9.565669028581244
3	1.7100720915357832	3.3066342656336705
4	0.6920587553482418	1.7842407195134722
5	0.3842099676570839	1.2381952107371472
6	0.2510525966162874	0.9708820143391635
7	0.16764724076025783	0.7563885357161948
8	0.13194900836797716	0.6803722713141653
9	0.10067951761243321	0.5840286110144511
>10	0.6457894908026525	8.25067343576118
>50	0.0830072895212255	3.767576651373652
>100	0.07345904898960062	8.827592342686527
>500	0.0026002936994090533	1.1382105573728885
>1k	6.500734248522633E-4	0.7223541324906533
>5k	3.2503671242613166E-4	1.4710353635842623
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7668	0.5345215307335939	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7386	0.5148638531557543	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6011	0.41901524794465733	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4575	0.31891445006601354	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.091242295514843E-4	0.0
3	0.0	0.0	0.0	6.970807651716143E-4	0.0
4	0.0	0.0	0.0	0.0018821180659633586	0.0
5	0.0	0.0	0.0	0.0020912422955148427	0.0
6	0.0	0.0	0.0	0.0020912422955148427	0.0
7	0.0	0.0	0.0	0.0023700746015834885	0.0
8	0.0	0.0	0.0	0.00243978267810065	0.0
9	0.0	0.0	0.0	0.004043068437995363	0.0
10	0.0	0.0	0.0	0.005785770350924399	0.0
11	0.0	6.970807651716143E-5	0.0	0.007667888416887757	0.0
12	0.0	6.970807651716143E-5	0.0	0.00968942263588544	0.0
13	0.0	6.970807651716143E-5	0.0	0.010525919554091377	0.0
14	0.0	6.970807651716143E-5	0.0	0.012338329543537573	0.0
15	6.970807651716143E-5	6.970807651716143E-5	0.0	0.014847820298155385	0.0
16	6.970807651716143E-5	6.970807651716143E-5	0.0	0.01993650988390817	0.0
17	6.970807651716143E-5	6.970807651716143E-5	0.0	0.027325565994727283	0.0
18	6.970807651716143E-5	6.970807651716143E-5	0.0	0.03004418097889658	0.0
19	6.970807651716143E-5	6.970807651716143E-5	0.0	0.03464491402902923	0.0
20	6.970807651716143E-5	6.970807651716143E-5	0.0	0.03861827439050743	0.0
21	6.970807651716143E-5	6.970807651716143E-5	0.0	0.04837740510291003	0.0
22	1.3941615303432285E-4	6.970807651716143E-5	0.0	0.06447997077837432	0.0
23	1.3941615303432285E-4	6.970807651716143E-5	0.0	0.09041137524275837	0.0
24	1.3941615303432285E-4	6.970807651716143E-5	0.0	0.13021468693405755	0.0
25	1.3941615303432285E-4	6.970807651716143E-5	0.0	0.14032235802904597	0.0
26	1.3941615303432285E-4	6.970807651716143E-5	0.0	0.15712200446968186	0.0
27	1.3941615303432285E-4	6.970807651716143E-5	0.0	0.18535377545913226	0.0
28	2.091242295514843E-4	6.970807651716143E-5	0.0	0.24606951010557984	0.0
29	2.091242295514843E-4	6.970807651716143E-5	0.0	0.3357838045831666	0.0
30	2.091242295514843E-4	6.970807651716143E-5	0.0	0.46732294497105026	0.0
31	2.091242295514843E-4	6.970807651716143E-5	0.0	0.8678655526386598	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGCTTA	90	2.152974E-6	18.5	25
CAGTCGG	290	0.0	18.5	10
AGTCGGT	290	0.0	18.5	11
GCTACGA	85	2.7238788E-5	17.411764	2
GGTATCA	3805	0.0	17.211563	1
CGAACTA	130	1.3938916E-8	17.076923	24
GCAGTCG	315	0.0	17.031746	9
CGCTTAT	100	5.879836E-6	16.65	26
GTCGGTG	315	0.0	16.444445	12
CTAGGAC	170	8.54925E-11	16.323528	3
TCGTTCT	210	0.0	15.857142	30
CGTCAAT	215	0.0	15.488372	24
GACGGAC	240	0.0	15.416666	7
GACACGC	380	0.0	15.092106	30
TCTAGCG	135	3.973837E-7	15.074073	28
CTTATAC	1475	0.0	15.050847	37
CTAGGTA	415	0.0	14.710843	4
TCGGTGA	355	0.0	14.591549	13
CGGACCA	255	0.0	14.509804	9
TAGACAG	195	6.730261E-10	14.23077	5
>>END_MODULE