FastQCFastQC Report
Fri 10 Feb 2017
ERR1630711.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630711.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1568518
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC32040.2042692528871202No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA31930.20356795395398714No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC23580.1503329894843413No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC21030.13407560512534764No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC20720.13209921722288173No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC19990.1274451424848169No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC19760.12597879017008412No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA18630.11877453749335361No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG18280.11654313179702114No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT18130.11558681507002151No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG17280.11016768695035696No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC17060.10876508908409083No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC17040.10863758018715755No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG15820.10085953747422727No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9800.023.0306131
GTACCGT450.003826419820.5555576
TTAACGG909.483847E-820.55555735
CTAGTAC1001.2907549E-820.3499983
TAACGGC951.67709E-719.47368436
GTCTAGA1800.018.51
CTTATAC7700.018.01948237
CTAGAAC2100.015.8571443
TAGAACT2450.015.8571434
CTAGCGG3050.015.77049329
TACCGCA5350.015.56074728
TCTAGCG3100.015.516129528
GTTCAAA6450.015.4883721
GTCCTAA2750.015.4727281
CCGATTT1201.936498E-615.41666620
CGAGCCG5400.015.41666615
GTATCAA14400.015.2881952
TCTTATA10450.015.04784737
GTGGGTA3500.014.89
ATTTGCG3250.014.816
Produced by FastQC (version 0.11.5)