FastQCFastQC Report
Fri 10 Feb 2017
ERR1630710.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630710.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1930106
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT80110.41505492444456416No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT68460.35469554521876No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT58390.30252224489224944No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44390.22998736856939464No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36600.019.0054651
CGAATTA1302.5914233E-715.65384615
CTTATAC13050.015.45210637
ATGCCGT1201.93706E-615.41666634
CGAACTA1101.4528063E-515.13636424
TACGTTA1353.9759107E-715.07407319
CGGGTTT750.004105748614.837
TTATGCG750.004105748614.84
ATTACGT1752.240995E-914.817
ATAGCGG1551.2113378E-714.32258137
TTGACGG3100.014.32258133
ACGAACC1956.730261E-1014.2307726
CCTACAC2750.014.1272743
TTACACT2500.014.0599994
TCTTATA20300.013.85221737
TACTCCG2850.013.6315795
ATACCGT2457.2759576E-1213.5918366
AAGACGG4500.013.5666675
GACGGAC4100.013.5365857
CTAGCGG2051.4242687E-913.53658529
Produced by FastQC (version 0.11.5)