##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630710.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1930106
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.952234229622622	33.0	31.0	34.0	30.0	34.0
2	32.12188864238544	33.0	31.0	34.0	30.0	34.0
3	32.22555963247614	34.0	31.0	34.0	30.0	34.0
4	35.80995914214038	37.0	35.0	37.0	35.0	37.0
5	35.7904208369903	37.0	35.0	37.0	35.0	37.0
6	35.82982489044643	37.0	35.0	37.0	35.0	37.0
7	35.808093441500105	37.0	35.0	37.0	35.0	37.0
8	35.79930584123359	37.0	35.0	37.0	35.0	37.0
9	37.518942482951715	39.0	37.0	39.0	35.0	39.0
10	37.4236658504766	39.0	37.0	39.0	34.0	39.0
11	37.53585813421646	39.0	37.0	39.0	35.0	39.0
12	37.465565621784506	39.0	37.0	39.0	35.0	39.0
13	37.51624159502121	39.0	37.0	39.0	35.0	39.0
14	38.774376640453944	40.0	38.0	41.0	35.0	41.0
15	38.75142194262906	40.0	38.0	41.0	35.0	41.0
16	38.72445451182474	40.0	38.0	41.0	35.0	41.0
17	38.72213805873874	40.0	38.0	41.0	35.0	41.0
18	38.711495638063404	40.0	38.0	41.0	35.0	41.0
19	38.74952256508192	40.0	38.0	41.0	34.0	41.0
20	38.73302968852488	40.0	38.0	41.0	34.0	41.0
21	38.6604963665208	40.0	38.0	41.0	34.0	41.0
22	38.603690160022296	40.0	38.0	41.0	34.0	41.0
23	38.57246182334027	40.0	38.0	41.0	34.0	41.0
24	38.52418934504115	40.0	38.0	41.0	34.0	41.0
25	38.479915092746204	40.0	38.0	41.0	34.0	41.0
26	38.28989910398703	40.0	38.0	41.0	34.0	41.0
27	38.16999273615024	40.0	38.0	41.0	33.0	41.0
28	38.08090747347555	40.0	38.0	41.0	33.0	41.0
29	37.97689919620995	40.0	37.0	41.0	33.0	41.0
30	37.90044899088444	40.0	37.0	41.0	33.0	41.0
31	37.84502457377989	40.0	37.0	41.0	33.0	41.0
32	37.74128001260034	40.0	37.0	41.0	33.0	41.0
33	37.70310594340415	40.0	37.0	41.0	33.0	41.0
34	37.6257848014565	40.0	37.0	41.0	33.0	41.0
35	37.56686057656937	40.0	37.0	41.0	32.0	41.0
36	37.51912951931138	40.0	37.0	41.0	32.0	41.0
37	37.33455001953261	40.0	36.0	41.0	32.0	41.0
38	37.220108118414224	40.0	36.0	41.0	31.0	41.0
39	37.15406045056593	40.0	36.0	41.0	31.0	41.0
40	37.049566707735224	40.0	36.0	41.0	31.0	41.0
41	36.91937230390455	39.0	36.0	41.0	31.0	41.0
42	36.82018604159564	39.0	35.0	41.0	30.0	41.0
43	35.99109530771885	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	8.0
11	3.0
12	2.0
13	3.0
14	2.0
15	6.0
16	9.0
17	34.0
18	62.0
19	137.0
20	280.0
21	507.0
22	1007.0
23	1741.0
24	2929.0
25	4632.0
26	7187.0
27	10606.0
28	15599.0
29	21395.0
30	29034.0
31	38254.0
32	48993.0
33	62749.0
34	83369.0
35	111029.0
36	154668.0
37	238343.0
38	431049.0
39	666468.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.23793615480186	19.954344476417358	12.452735756481768	24.354983612299012
2	18.536598508061218	21.544671639795947	35.21153760467042	24.707192247472417
3	20.40748021093142	24.069040767709133	29.839397421696013	25.68408159966344
4	14.44832563600134	15.859647086740313	34.678302642445544	35.0137246348128
5	13.971771498560182	37.74803041905471	34.14967882592977	14.130519256455345
6	34.11444760028724	34.844977426110276	16.049947515835917	14.990627457766568
7	28.084675142194264	31.322217536238945	21.95387196350874	18.639235358058055
8	26.79567857931119	33.60364663909651	20.59689985938596	19.003774922206347
9	26.411658219807617	14.209375029143478	20.79683706490732	38.58212968614159
10	17.06812993690502	27.151203094545068	33.27584080874315	22.50482615980677
11	34.46396208291151	22.281833225739934	22.021433019740886	21.232771671607672
12	21.04599436507632	26.622579278029292	29.50418267183253	22.827243685061855
13	29.249533445313364	20.508303689020188	26.348760119910512	23.89340274575593
14	22.51860778630811	22.070704925014482	26.037740932363302	29.37294635631411
15	25.396221761913594	28.09172138732277	23.52026261770079	22.991794233062848
16	24.5865771102727	27.053954549646498	25.10064214089796	23.25882619918284
17	22.922575236800466	27.225862206531666	26.327414142021215	23.524148414646657
18	22.4806305974905	25.534089837552965	28.31098395632157	23.674295608634964
19	23.255665751000205	26.119239046974624	28.120579905974076	22.504515296051096
20	23.89210748010731	25.23068681201965	28.11332641834179	22.76387928953125
21	24.225042562429213	25.993131983424746	27.032556761131254	22.749268693014788
22	23.950912540554768	25.727654336083095	27.305495138608965	23.01593798475317
23	23.16577431498581	25.940129713083117	27.622109873758227	23.271986098172846
24	23.46736396861105	26.423367421271166	27.336426082298072	22.772842527819716
25	23.59771950348841	25.864071714196008	27.458854591405863	23.079354190909722
26	23.277115350141393	26.556106244941986	27.3204165988811	22.846361806035524
27	23.65538473016508	25.897385946678575	27.00136676431243	23.44586255884392
28	22.821440894956027	26.212083688667875	27.84049166211597	23.12598375426013
29	22.522804447009644	26.58164888353282	27.890074431145234	23.0054722383123
30	22.454103557006714	27.120790257115413	27.78230832917985	22.642797856698028
31	23.148987672179665	26.7310707287579	27.204723471146142	22.91521812791629
32	22.46886958540101	26.49486608507512	27.329276215917673	23.706988113606194
33	22.4435859999399	26.193017378320153	27.947169740936506	23.416226880803436
34	23.099560335028233	26.014477961314043	27.610089808539012	23.275871895118712
35	23.069199308224523	26.080432888141896	27.97898146526667	22.871386338366907
36	22.474568754254946	26.590145826187783	27.558693667601673	23.37659175195559
37	23.23297269683634	25.53212103376706	27.617705970552915	23.61720029884369
38	22.479801627475382	25.644912766449096	28.451908858891688	23.423376747183834
39	22.632539352760936	25.596780694946286	28.297824057331567	23.47285589496121
40	22.161114467288325	25.437929315799234	29.045503200342367	23.355453016570074
41	21.636790932725976	25.675014740123082	29.29466050051137	23.393533826639572
42	22.325665015289314	25.362389423171578	29.064051404430636	23.24789415710847
43	21.550837104283392	25.108465545415644	29.085034707938323	24.255662642362648
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	609.0
1	528.5
2	448.0
3	1122.5
4	1797.0
5	1797.0
6	2390.0
7	2983.0
8	2844.5
9	2706.0
10	4037.5
11	5369.0
12	5369.0
13	8891.0
14	12413.0
15	16446.0
16	20479.0
17	20433.5
18	20388.0
19	20388.0
20	24026.0
21	27664.0
22	27681.5
23	27699.0
24	30621.0
25	33543.0
26	33543.0
27	37381.5
28	41220.0
29	49060.5
30	56901.0
31	61620.5
32	66340.0
33	66340.0
34	71762.5
35	77185.0
36	83521.0
37	89857.0
38	97728.5
39	105600.0
40	105600.0
41	110579.5
42	115559.0
43	113617.5
44	111676.0
45	115208.0
46	118740.0
47	118740.0
48	122367.5
49	125995.0
50	127691.5
51	129388.0
52	133748.0
53	138108.0
54	138108.0
55	130102.5
56	122097.0
57	116186.0
58	110275.0
59	101699.5
60	93124.0
61	93124.0
62	86196.0
63	79268.0
64	70343.0
65	61418.0
66	53359.0
67	45300.0
68	45300.0
69	38633.0
70	31966.0
71	27380.5
72	22795.0
73	17828.0
74	12861.0
75	12861.0
76	9977.5
77	7094.0
78	5773.0
79	4452.0
80	3573.5
81	2695.0
82	2695.0
83	2069.0
84	1443.0
85	1281.5
86	1120.0
87	1030.0
88	940.0
89	940.0
90	727.0
91	514.0
92	282.5
93	51.0
94	35.5
95	20.0
96	20.0
97	12.5
98	5.0
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1930106.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.77394392867856
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.7344957373432	50.10993893411475
2	8.248400286480017	9.530852313047854
3	2.0280658897809007	3.5150809499960203
4	0.843733335516313	1.9498320966750562
5	0.469385930271923	1.3559138208220352
6	0.2822325629811994	0.9783412961113833
7	0.19882770802672864	0.8040942598502725
8	0.1539666103842176	0.711620665218119
9	0.09838520242757333	0.511569105161608
>10	0.7102269300583676	8.080025807920164
>50	0.11114407662506755	4.543238387985005
>100	0.11178372431423662	12.78908942440227
>500	0.007823312536001676	2.9969111599186005
>1k	0.0012589238563680857	1.0482902850750186
>5k	2.697693977931612E-4	1.075201493701684
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8011	0.41505492444456416	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6846	0.35469554521876	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5839	0.30252224489224944	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4439	0.22998736856939464	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.0724250377958517E-4	0.0
3	0.0	5.181062594489629E-5	0.0	4.662956335040666E-4	0.0
4	0.0	5.181062594489629E-5	0.0	0.0010362125188979258	0.0
5	0.0	1.0362125188979258E-4	0.0	0.0013988869005121998	0.0
6	0.0	1.0362125188979258E-4	0.0	0.0015025081524019923	0.0
7	0.0	1.0362125188979258E-4	0.0	0.0017097506561815775	0.0
8	0.0	1.0362125188979258E-4	0.0	0.0018651825340162665	0.0
9	0.0	1.5543187783468887E-4	0.0	0.0029013950529141923	0.0
10	0.0	1.5543187783468887E-4	0.0	0.00461114570909577	0.0
11	0.0	1.5543187783468887E-4	0.0	0.005595547602048799	0.0
12	0.0	1.5543187783468887E-4	0.0	0.00642451761716714	0.0
13	0.0	1.5543187783468887E-4	0.0	0.006994434502561	0.0
14	0.0	1.5543187783468887E-4	0.0	0.007978836395514029	0.0
15	0.0	1.5543187783468887E-4	0.0	0.009429533921971125	0.0
16	0.0	1.5543187783468887E-4	0.0	0.011968254593271043	0.0
17	0.0	1.5543187783468887E-4	0.0	0.015491377157523991	0.0
18	0.0	1.5543187783468887E-4	0.0	0.01694207468398109	0.0
19	0.0	1.5543187783468887E-4	0.0	0.019118120973666733	0.0
20	0.0	1.5543187783468887E-4	0.0	0.021553220393076857	0.0
21	0.0	1.5543187783468887E-4	0.0	0.025387206712999183	0.0
22	0.0	1.5543187783468887E-4	0.0	0.03253707309339487	0.0
23	0.0	3.1086375566937775E-4	0.0	0.044453517060721016	0.0
24	5.181062594489629E-5	3.1086375566937775E-4	0.0	0.06087748548525314	0.0
25	5.181062594489629E-5	3.1086375566937775E-4	0.0	0.06595492682785298	0.0
26	5.181062594489629E-5	3.1086375566937775E-4	0.0	0.0753844607498241	0.0
27	5.181062594489629E-5	3.1086375566937775E-4	0.0	0.09237834605975008	0.0
28	5.181062594489629E-5	3.6267438161427404E-4	0.0	0.1299928604957448	0.0
29	5.181062594489629E-5	3.6267438161427404E-4	0.0	0.18403134335627164	0.0
30	5.181062594489629E-5	3.6267438161427404E-4	0.0	0.2658921323492078	0.0
31	5.181062594489629E-5	3.6267438161427404E-4	0.0	0.5241162920585709	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3660	0.0	19.005465	1
CGAATTA	130	2.5914233E-7	15.653846	15
CTTATAC	1305	0.0	15.452106	37
ATGCCGT	120	1.93706E-6	15.416666	34
CGAACTA	110	1.4528063E-5	15.136364	24
TACGTTA	135	3.9759107E-7	15.074073	19
CGGGTTT	75	0.0041057486	14.8	37
TTATGCG	75	0.0041057486	14.8	4
ATTACGT	175	2.240995E-9	14.8	17
ATAGCGG	155	1.2113378E-7	14.322581	37
TTGACGG	310	0.0	14.322581	33
ACGAACC	195	6.730261E-10	14.23077	26
CCTACAC	275	0.0	14.127274	3
TTACACT	250	0.0	14.059999	4
TCTTATA	2030	0.0	13.852217	37
TACTCCG	285	0.0	13.631579	5
ATACCGT	245	7.2759576E-12	13.591836	6
AAGACGG	450	0.0	13.566667	5
GACGGAC	410	0.0	13.536585	7
CTAGCGG	205	1.4242687E-9	13.536585	29
>>END_MODULE