FastQCFastQC Report
Fri 10 Feb 2017
ERR1630709.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630709.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2026288
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT65860.3250278341479592No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT54070.26684262059490066No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT49440.2439929565787292No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30500.15052154481495228No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG30310.14958386961774436No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG24450.120663992482806No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA24390.12036788452579297No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC23200.11449507671170141No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG3600.019.52777928
CTAGCGG3700.019.029
CGCAATA3750.018.74666636
GGTATCA32100.017.981311
AAGACGG3950.017.3291135
GCAATAC4450.016.21348237
ATAATAC1601.0977601E-815.031253
GCGCAAG4600.014.8804341
CGACACT750.00410584414.84
GTGTCGA1001.09401786E-414.89
CGAATTA750.00410584414.815
TTCGGGC3250.014.835
ACGCTTC3750.014.79999931
TAATACT3150.014.682544
GCTTAGG10000.014.6151
TCGGTTG1152.2113798E-514.4782624
ATACCGT3450.014.478266
CAAGACG5000.014.4299994
GACGGAC4500.014.3888897
CGGCGCA6500.014.23076933
Produced by FastQC (version 0.11.5)