##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630709.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2026288
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.932398059900667	33.0	31.0	34.0	30.0	34.0
2	32.10480839841128	33.0	31.0	34.0	30.0	34.0
3	32.2039226408092	34.0	31.0	34.0	30.0	34.0
4	35.80087677566072	37.0	35.0	37.0	35.0	37.0
5	35.7766107285835	37.0	35.0	37.0	35.0	37.0
6	35.81108953909809	37.0	35.0	37.0	35.0	37.0
7	35.791798105698696	37.0	35.0	37.0	35.0	37.0
8	35.78291930860766	37.0	35.0	37.0	35.0	37.0
9	37.49886245193181	39.0	37.0	39.0	35.0	39.0
10	37.40296591600009	39.0	37.0	39.0	34.0	39.0
11	37.50893456408961	39.0	37.0	39.0	35.0	39.0
12	37.44014671162243	39.0	37.0	39.0	34.0	39.0
13	37.48369086724099	39.0	37.0	39.0	35.0	39.0
14	38.74609976469288	40.0	38.0	41.0	35.0	41.0
15	38.71966176575097	40.0	38.0	41.0	35.0	41.0
16	38.69689303790972	40.0	38.0	41.0	35.0	41.0
17	38.683196070844815	40.0	38.0	41.0	35.0	41.0
18	38.66907566940139	40.0	38.0	41.0	34.0	41.0
19	38.69984720829418	40.0	38.0	41.0	34.0	41.0
20	38.69157197792219	40.0	38.0	41.0	34.0	41.0
21	38.61425769683283	40.0	38.0	41.0	34.0	41.0
22	38.5564791382074	40.0	38.0	41.0	34.0	41.0
23	38.52561333828162	40.0	38.0	41.0	34.0	41.0
24	38.47188306894183	40.0	38.0	41.0	34.0	41.0
25	38.43436915186785	40.0	38.0	41.0	34.0	41.0
26	38.24382960368911	40.0	38.0	41.0	34.0	41.0
27	38.128337630188796	40.0	38.0	41.0	33.0	41.0
28	38.03187848913876	40.0	37.0	41.0	33.0	41.0
29	37.934265514082895	40.0	37.0	41.0	33.0	41.0
30	37.862858586735946	40.0	37.0	41.0	33.0	41.0
31	37.822271069068165	40.0	37.0	41.0	33.0	41.0
32	37.72390499277497	40.0	37.0	41.0	33.0	41.0
33	37.681150458375114	40.0	37.0	41.0	33.0	41.0
34	37.618454040096964	40.0	37.0	41.0	32.0	41.0
35	37.563939578184346	40.0	37.0	41.0	32.0	41.0
36	37.52492044566222	40.0	37.0	41.0	32.0	41.0
37	37.34307265304833	40.0	36.0	41.0	31.0	41.0
38	37.22731270184693	40.0	36.0	41.0	31.0	41.0
39	37.17171645886468	39.0	36.0	41.0	31.0	41.0
40	37.06653989956018	39.0	36.0	41.0	31.0	41.0
41	36.941984555008965	39.0	35.0	41.0	31.0	41.0
42	36.84743679082144	39.0	35.0	41.0	31.0	41.0
43	36.00015792424374	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	0.0
11	0.0
12	2.0
13	2.0
14	1.0
15	5.0
16	6.0
17	35.0
18	72.0
19	161.0
20	307.0
21	620.0
22	1209.0
23	1881.0
24	3067.0
25	5080.0
26	7673.0
27	11416.0
28	16313.0
29	22303.0
30	30542.0
31	39999.0
32	51617.0
33	66403.0
34	87926.0
35	117766.0
36	165083.0
37	252527.0
38	460931.0
39	683339.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.50911617696991	19.38085800241624	12.207050527861785	24.902975292752068
2	19.178418862471673	21.385854330677574	34.185663637153255	25.250063169697494
3	20.882224047124595	23.776235164991355	29.188101592665998	26.153439195218052
4	14.888604186571703	15.513243921890668	33.939597924875436	35.65855396666219
5	14.395090924883332	37.27959697732997	33.80817534328783	14.517136754498866
6	35.0273011536366	34.0980156818774	15.688638535094714	15.186044629391283
7	28.716401617144253	30.59989498037791	21.513772968107197	19.169930434370634
8	27.084698720024004	32.9820341432215	20.258966148938352	19.674300987816146
9	26.691368650458376	14.021649439763745	20.10508871394392	39.181893195833965
10	17.503730960258366	26.668222878485192	32.404722329698444	23.423323831558
11	35.654112347307	21.810078330424894	21.312370206012176	21.223439116255932
12	21.759345167123332	26.137251960234675	28.13420402233049	23.969198850311503
13	29.42982438824096	20.02913702297008	25.8793912810025	24.661647307786456
14	23.045440727083218	21.53909019843181	24.719783170013347	30.695685904471624
15	25.934023199071405	27.843277954565192	22.697661931571425	23.525036914791976
16	25.75216356213924	26.290241071358068	23.933962003426956	24.02363336307573
17	24.039228382145083	26.45260693445354	25.025366581650783	24.482798101750593
18	23.359167107538514	24.694663344993405	27.3157616291465	24.63040791832158
19	24.122730826022757	25.57943392054831	26.796437623871828	23.501397629557104
20	24.624781867138335	24.47514864619442	26.96289964703932	23.93716983962793
21	24.934165330890774	25.579236515243636	25.911025481076727	23.575572672788862
22	24.817498795827643	25.012091074911364	26.101176140805254	24.06923398845574
23	23.73571772620674	25.335292909991075	26.604855775684406	24.324133588117782
24	24.519466137094035	25.832063359206586	26.105420354855774	23.5430501488436
25	24.55238347164865	25.15866451363281	26.250809361749166	24.03814265296937
26	24.2889460925594	26.037463578721287	26.132070070986945	23.541520257732365
27	24.83709127231667	24.80703631467985	25.948729894269718	24.407142518733764
28	23.48012720797833	25.347877498164127	27.035051285898152	24.136944007959382
29	23.36360872689371	25.846276541143215	26.731343224655134	24.058771507307945
30	22.93553532370522	26.69832718744818	26.839669385595734	23.52646810325087
31	24.143655788318345	26.130934990485066	25.99299803384317	23.732411187353428
32	23.386310336931373	25.636977566861173	26.10749311055487	24.869218985652584
33	23.168621637200634	25.445987934587777	26.779707524300594	24.605682903910996
34	24.091442085231716	25.408678332004136	26.518688360193615	23.981191222570533
35	24.088530356987754	25.390122233364654	26.86903342466619	23.652313984981404
36	23.166104719566025	26.40789463294458	26.429855973089712	23.99614467439969
37	24.509743925838777	25.088042765885206	26.335002724193206	24.067210584082815
38	23.332369337428833	25.230026531272948	27.22021746168363	24.217386669614584
39	23.929372330093255	25.059172240076432	26.880976445599046	24.130478984231267
40	23.259082618068113	24.90588702099603	27.72152823290667	24.113502128029186
41	22.604239871133817	25.507232930363305	27.938871473354233	23.94965572514865
42	23.72288638140284	24.84775115876914	27.811890511121817	23.617471948706207
43	22.73354034569617	24.42777137307234	27.737221954628367	25.101466326603127
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	144.0
1	166.5
2	189.0
3	587.0
4	985.0
5	985.0
6	1450.0
7	1915.0
8	1885.0
9	1855.0
10	2752.5
11	3650.0
12	3650.0
13	6153.5
14	8657.0
15	12131.0
16	15605.0
17	15688.0
18	15771.0
19	15771.0
20	18277.0
21	20783.0
22	20924.0
23	21065.0
24	23349.0
25	25633.0
26	25633.0
27	29506.0
28	33379.0
29	42712.0
30	52045.0
31	55414.5
32	58784.0
33	58784.0
34	65111.0
35	71438.0
36	78093.5
37	84749.0
38	96758.5
39	108768.0
40	108768.0
41	116415.0
42	124062.0
43	120539.5
44	117017.0
45	122802.5
46	128588.0
47	128588.0
48	136415.0
49	144242.0
50	145651.0
51	147060.0
52	154011.0
53	160962.0
54	160962.0
55	147396.0
56	133830.0
57	126212.0
58	118594.0
59	110282.0
60	101970.0
61	101970.0
62	95471.0
63	88972.0
64	80943.5
65	72915.0
66	64421.5
67	55928.0
68	55928.0
69	47434.5
70	38941.0
71	34063.5
72	29186.0
73	22256.0
74	15326.0
75	15326.0
76	11868.5
77	8411.0
78	6966.0
79	5521.0
80	4498.5
81	3476.0
82	3476.0
83	2684.5
84	1893.0
85	1774.5
86	1656.0
87	1521.0
88	1386.0
89	1386.0
90	1102.5
91	819.0
92	449.5
93	80.0
94	54.0
95	28.0
96	28.0
97	17.0
98	6.0
99	5.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2026288.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.762885885913654
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.13994632734574	42.86572319306433
2	8.375676821048314	8.335956997262386
3	2.11697645840402	3.160405737681746
4	0.8883559372038237	1.76828620516591
5	0.4823195708303064	1.2000806881885695
6	0.32195978275078013	0.9612988757328373
7	0.2187186741430091	0.7618850695747829
8	0.1706263450946049	0.6792687472058677
9	0.12651318958966412	0.5666095274952073
>10	0.850413260263209	8.501458058593105
>50	0.131866254378884	4.671025976509932
>100	0.15570771991777674	16.63154431458246
>500	0.014942613592830426	5.060857035840139
>1k	0.005777810589227765	4.2410765205919425
>5k	1.992348479044057E-4	0.5945230525108315
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6586	0.3250278341479592	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5407	0.26684262059490066	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4944	0.2439929565787292	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3050	0.15052154481495228	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	3031	0.14958386961774436	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	2445	0.120663992482806	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	2439	0.12036788452579297	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	2320	0.11449507671170141	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	4.935132616883681E-5	0.0	7.896212187013889E-4	0.0
3	0.0	4.935132616883681E-5	0.0	0.0014805397850651042	0.0
4	0.0	4.935132616883681E-5	0.0	0.0029610795701302084	0.0
5	0.0	1.4805397850651042E-4	0.0	0.00340524150564974	4.935132616883681E-5
6	0.0	1.4805397850651042E-4	0.0	0.00340524150564974	4.935132616883681E-5
7	0.0	1.4805397850651042E-4	0.0	0.003997457419675781	4.935132616883681E-5
8	0.0	1.4805397850651042E-4	0.0	0.004293565376688803	4.935132616883681E-5
9	0.0	1.4805397850651042E-4	0.0	0.005774105161753907	4.935132616883681E-5
10	0.0	1.4805397850651042E-4	0.0	0.008587130753377605	4.935132616883681E-5
11	0.0	1.9740530467534723E-4	0.0	0.010018319212273872	4.935132616883681E-5
12	0.0	1.9740530467534723E-4	0.0	0.010758589104806425	4.935132616883681E-5
13	0.0	1.9740530467534723E-4	0.0	0.011696264302014323	4.935132616883681E-5
14	0.0	1.9740530467534723E-4	0.0	0.012436534194546876	4.935132616883681E-5
15	0.0	1.9740530467534723E-4	0.0	0.013670317348767795	4.935132616883681E-5
16	0.0	1.9740530467534723E-4	0.0	0.014805397850651042	4.935132616883681E-5
17	0.0	1.9740530467534723E-4	0.0	0.016582045592729168	4.935132616883681E-5
18	0.0	1.9740530467534723E-4	0.0	0.017569072116105904	4.935132616883681E-5
19	0.0	2.4675663084418407E-4	0.0	0.01855609863948264	4.935132616883681E-5
20	0.0	2.4675663084418407E-4	0.0	0.020036638424547743	4.935132616883681E-5
21	0.0	2.4675663084418407E-4	0.0	0.022602907385327258	4.935132616883681E-5
22	0.0	2.4675663084418407E-4	0.0	0.026995175414353735	4.935132616883681E-5
23	0.0	2.9610795701302085E-4	0.0	0.032522523945263455	4.935132616883681E-5
24	0.0	2.9610795701302085E-4	0.0	0.0420966812220178	4.935132616883681E-5
25	0.0	2.9610795701302085E-4	0.0	0.045551274053836376	9.870265233767362E-5
26	0.0	2.9610795701302085E-4	0.0	0.052065649108122836	9.870265233767362E-5
27	0.0	3.454592831818577E-4	0.0	0.06351515677929297	9.870265233767362E-5
28	0.0	3.454592831818577E-4	0.0	0.0847362270318928	9.870265233767362E-5
29	0.0	3.9481060935069446E-4	0.0	0.1163704271061172	9.870265233767362E-5
30	0.0	3.9481060935069446E-4	0.0	0.1651788886870968	9.870265233767362E-5
31	0.0	3.9481060935069446E-4	0.0	0.31885891837685465	9.870265233767362E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	360	0.0	19.527779	28
CTAGCGG	370	0.0	19.0	29
CGCAATA	375	0.0	18.746666	36
GGTATCA	3210	0.0	17.98131	1
AAGACGG	395	0.0	17.329113	5
GCAATAC	445	0.0	16.213482	37
ATAATAC	160	1.0977601E-8	15.03125	3
GCGCAAG	460	0.0	14.880434	1
CGACACT	75	0.004105844	14.8	4
GTGTCGA	100	1.09401786E-4	14.8	9
CGAATTA	75	0.004105844	14.8	15
TTCGGGC	325	0.0	14.8	35
ACGCTTC	375	0.0	14.799999	31
TAATACT	315	0.0	14.68254	4
GCTTAGG	1000	0.0	14.615	1
TCGGTTG	115	2.2113798E-5	14.47826	24
ATACCGT	345	0.0	14.47826	6
CAAGACG	500	0.0	14.429999	4
GACGGAC	450	0.0	14.388889	7
CGGCGCA	650	0.0	14.230769	33
>>END_MODULE