FastQCFastQC Report
Fri 10 Feb 2017
ERR1630708.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630708.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences297576
Sequences flagged as poor quality0
Sequence length43
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT89753.0160362394816786No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT68112.288827055945372No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT53791.8076054520525848No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45161.5175955050138452No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13940.4684517568621125No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT13810.4640831249831976No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGT12820.4308143129822297No Hit
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT12490.41972470898190717TruSeq Adapter, Index 5 (95% over 21bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11320.38040702207167243No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT9410.3162217383122295No Hit
CATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTC7500.2520364545527865No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA7390.2483399198860123No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7080.23792241309783047No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA6700.22515256606715595No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG6370.21406296206683334No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6120.2056617469150738No Hit
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCC5920.19894077479366618No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA5910.19860472618759578No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5570.18717907358120278No Hit
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATG4810.16163937951985374No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA4620.15525445600451648No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA4480.15054977551953114No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4110.138115977094927No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA3980.13374734521601203No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA3880.13038685915530823No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG3860.12971476194316744No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC3780.1270263730946044No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA3660.12299378982175982No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA3440.11560072048821142No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT3240.10887974836680378No Hit
GTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAA3170.1065274081243111No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA3120.1048471650939592No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC3120.1048471650939592No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT303.5931263E-430.8333321
TTTACCT250.005489879829.63
TAAGGAA250.005489879829.621
GCCGTCT1800.024.66666636
CCGTCTT1900.023.36842237
AAATAAC400.001927843123.12500233
CTATACC502.6955857E-422.24
CGTTATA502.6955857E-422.22
CGTCTTC806.9245107E-720.812537
CCCCTAT450.003819246820.5555551
ACCTTCT450.003819246820.5555558
TAACTAG450.003819246820.55555526
AGTCGGT3600.019.0138911
AAGGGTC500.007022933618.55
GGCCTAA500.007022933618.56
CAGTCGG3650.018.24657410
ATGCCGT2450.018.12244834
GCAGTCG3750.017.7600029
GTCGGTG3750.017.76000212
GGCAGTC3800.017.5263168
Produced by FastQC (version 0.11.5)