##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630708.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	297576
Sequences flagged as poor quality	0
Sequence length	43
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.560549237841762	31.0	31.0	34.0	30.0	34.0
2	31.630981665188052	31.0	31.0	34.0	30.0	34.0
3	31.595323547597925	31.0	31.0	34.0	28.0	34.0
4	35.33078944538538	37.0	35.0	37.0	33.0	37.0
5	35.364989112025164	37.0	35.0	37.0	33.0	37.0
6	35.46804849853483	37.0	35.0	37.0	33.0	37.0
7	35.40617186869909	37.0	35.0	37.0	33.0	37.0
8	35.50136435734065	37.0	35.0	37.0	33.0	37.0
9	36.980579751055195	39.0	37.0	39.0	33.0	39.0
10	36.90129916391107	39.0	37.0	39.0	33.0	39.0
11	37.109931580503805	39.0	37.0	39.0	33.0	39.0
12	37.06503212624674	39.0	37.0	39.0	33.0	39.0
13	37.145068822754524	39.0	37.0	39.0	33.0	39.0
14	38.16943234669463	40.0	37.0	41.0	33.0	41.0
15	38.098764685324085	40.0	37.0	41.0	33.0	41.0
16	37.952059305858	40.0	37.0	41.0	33.0	41.0
17	38.08706347286071	40.0	37.0	41.0	33.0	41.0
18	38.180552195069495	40.0	38.0	41.0	33.0	41.0
19	38.28437105142888	40.0	38.0	41.0	34.0	41.0
20	38.308912681130195	40.0	38.0	41.0	34.0	41.0
21	38.242778315455546	40.0	37.0	41.0	34.0	41.0
22	38.129862623329835	40.0	37.0	41.0	33.0	41.0
23	38.049372261203864	40.0	37.0	41.0	33.0	41.0
24	37.931977041159236	40.0	37.0	41.0	33.0	41.0
25	37.81216563163696	40.0	37.0	41.0	33.0	41.0
26	37.405718203080895	39.0	36.0	41.0	32.0	41.0
27	37.12318869801328	39.0	35.0	41.0	32.0	41.0
28	36.932595370594406	39.0	35.0	41.0	31.0	41.0
29	36.686513697341184	39.0	35.0	40.0	31.0	41.0
30	36.40780170443853	38.0	35.0	40.0	31.0	41.0
31	36.11101701750141	38.0	35.0	40.0	30.0	41.0
32	35.774797026641934	38.0	35.0	40.0	30.0	41.0
33	35.52168857703578	37.0	35.0	40.0	30.0	41.0
34	35.22775358227814	37.0	35.0	40.0	28.0	41.0
35	34.936483452966634	37.0	34.0	40.0	26.0	41.0
36	34.62944256257225	37.0	34.0	40.0	24.0	41.0
37	34.101782401806595	36.0	33.0	40.0	21.0	41.0
38	33.67621380756513	36.0	33.0	40.0	20.0	41.0
39	33.23768717907358	35.0	33.0	40.0	16.0	41.0
40	32.743638599887085	35.0	33.0	40.0	12.0	41.0
41	32.209496061510336	35.0	31.0	40.0	10.0	41.0
42	31.71759483291663	35.0	31.0	40.0	8.0	41.0
43	30.79660322068984	35.0	28.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	0.0
10	2.0
11	2.0
12	0.0
13	3.0
14	5.0
15	3.0
16	3.0
17	14.0
18	25.0
19	55.0
20	124.0
21	289.0
22	517.0
23	808.0
24	1347.0
25	2140.0
26	2914.0
27	4143.0
28	5599.0
29	7223.0
30	8790.0
31	10568.0
32	12747.0
33	15523.0
34	19206.0
35	23187.0
36	32215.0
37	50498.0
38	58496.0
39	41129.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.50517514853348	21.459391886442454	13.491679436513698	17.543753528510365
2	17.78806086512353	25.093085463881497	32.358120278517085	24.76073339247789
3	24.757036857811112	27.788867381778097	29.31251176170121	18.141583998709574
4	14.42555851278329	18.451756862112536	30.29511788585101	36.827566739253164
5	11.183697610022314	43.96624727800629	30.299150469123852	14.550904642847543
6	30.1408715756647	30.662082963679865	18.221563566954323	20.975481893701104
7	24.579267145199882	36.82991907949566	21.66774202220609	16.923071753098366
8	32.472040755974945	30.168763609968547	19.94885340215609	17.410342231900422
9	28.285211172944052	12.374653869935749	23.859787079603194	35.480347877517005
10	21.363281985106326	30.90101352259591	27.09391886442455	20.641785627873215
11	27.84868403365863	25.217423448127533	19.904831034760868	27.029061483452963
12	20.167284996101838	33.21974890448154	28.28890770761083	18.324058391805792
13	29.324945560125816	20.835685673575828	30.908406591929456	18.9309621743689
14	22.66110170175014	24.451232626287066	31.58083985267629	21.3068258192865
15	28.262023819125197	24.57825899938167	29.20934483963761	17.950372341855527
16	21.285990805710135	25.549439470925073	33.32896470145442	19.835605021910368
17	17.78537247627497	31.54387450600855	31.6477135252843	19.023039492432186
18	17.056819098314378	24.584979971503078	35.97064279377369	22.38755813640885
19	18.04917063204022	28.409549157190096	37.961394736134636	15.579885474635052
20	18.798895072183242	24.056039465548295	39.421525929510445	17.723539532758018
21	21.168037744979433	25.163991719762347	37.064144957926715	16.603825577331506
22	20.578944538538053	26.259174126945723	35.84428851789123	17.317592816624998
23	18.83384412721456	27.014275344785872	36.486813452697795	17.66506707530177
24	17.915423286824208	28.663937951985375	36.30501115681372	17.115627604376694
25	16.628693174180714	28.485160093555933	36.68004140118827	18.206105331075086
26	18.406390300293037	28.269080839852677	36.43371777293868	16.890811086915612
27	17.000026883888488	29.465749926069307	35.776406699465014	17.7578164905772
28	16.4767992042369	28.784579401564642	37.95870634728607	16.779915046912386
29	16.412277871871385	30.365352044519717	36.92804527246821	16.294324811140683
30	15.478062746995725	30.484313251068635	37.83100787697933	16.206616124956312
31	15.636677689060946	30.908070543323383	36.492190230394925	16.963061537220746
32	15.42026238675162	31.58856897061591	36.64307605452052	16.348092588111946
33	15.207879667715138	31.487754388794798	36.85545877355701	16.448907169933058
34	15.992217114283411	31.968975992687582	35.147323708901254	16.891483184127754
35	15.240812431110035	31.779444578863885	35.499502648063014	17.48024034196306
36	15.820496276581444	32.42163346506439	33.87437158910665	17.88349866924752
37	15.282818506868834	32.159179503723415	34.55285372476275	18.005148264644998
38	15.64709519584913	30.909078689141595	35.0276904051402	18.416135709869074
39	16.06984434228567	31.58856897061591	34.36634674839369	17.975239938704735
40	15.274081243111004	31.399373605398285	34.94972712853187	18.37681802295884
41	15.230394924321855	32.06340565099336	33.57091969782509	19.135279726859693
42	15.076820711347688	31.72970938516547	34.201010834207054	18.99245906927978
43	14.855028631341238	31.925625722504503	33.54907653843052	19.670269107723744
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	80.0
1	113.5
2	147.0
3	492.5
4	838.0
5	838.0
6	1397.0
7	1956.0
8	2122.5
9	2289.0
10	3334.5
11	4380.0
12	4380.0
13	8195.0
14	12010.0
15	15657.5
16	19305.0
17	17377.5
18	15450.0
19	15450.0
20	17119.0
21	18788.0
22	13727.0
23	8666.0
24	7564.5
25	6463.0
26	6463.0
27	6853.0
28	7243.0
29	7408.5
30	7574.0
31	8042.0
32	8510.0
33	8510.0
34	8932.0
35	9354.0
36	9559.5
37	9765.0
38	10886.5
39	12008.0
40	12008.0
41	13035.0
42	14062.0
43	14850.0
44	15638.0
45	16417.5
46	17197.0
47	17197.0
48	18588.5
49	19980.0
50	18677.5
51	17375.0
52	15460.0
53	13545.0
54	13545.0
55	11812.5
56	10080.0
57	10172.5
58	10265.0
59	9415.0
60	8565.0
61	8565.0
62	7417.0
63	6269.0
64	5739.5
65	5210.0
66	4704.5
67	4199.0
68	4199.0
69	3858.0
70	3517.0
71	3064.0
72	2611.0
73	2232.5
74	1854.0
75	1854.0
76	1496.5
77	1139.0
78	921.0
79	703.0
80	510.5
81	318.0
82	318.0
83	236.0
84	154.0
85	98.0
86	42.0
87	30.0
88	18.0
89	18.0
90	13.5
91	9.0
92	4.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	297576.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.89567998072718
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.30140865132473	37.31383433443524
2	9.588222065688502	8.801159430219728
3	3.1746380461463692	4.371065154617242
4	1.4906183505565158	2.7365177116216497
5	0.9203367574115033	2.1119740646329253
6	0.6032086646609303	1.6610803100927898
7	0.42365669783668153	1.3610808557922778
8	0.3560329300627967	1.3072298736610188
9	0.25764833233338047	1.0642450867506785
>10	1.6258548151796985	14.22489809821726
>50	0.15854810449022982	4.996311955115398
>100	0.08588022326554115	6.940463818979667
>500	0.007340190022695826	2.2897938344629063
>1k	0.004404114013617495	3.690216516508264
>5k	0.0022020570068087475	7.130128954892954
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8975	3.0160362394816786	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6811	2.288827055945372	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5379	1.8076054520525848	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4516	1.5175955050138452	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1394	0.4684517568621125	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1381	0.4640831249831976	No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGT	1282	0.4308143129822297	No Hit
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT	1249	0.41972470898190717	TruSeq Adapter, Index 5 (95% over 21bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1132	0.38040702207167243	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	941	0.3162217383122295	No Hit
CATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTC	750	0.2520364545527865	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	739	0.2483399198860123	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	708	0.23792241309783047	No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	670	0.22515256606715595	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	637	0.21406296206683334	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	612	0.2056617469150738	No Hit
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCC	592	0.19894077479366618	No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA	591	0.19860472618759578	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	557	0.18717907358120278	No Hit
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATG	481	0.16163937951985374	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	462	0.15525445600451648	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	448	0.15054977551953114	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	411	0.138115977094927	No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	398	0.13374734521601203	No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA	388	0.13038685915530823	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	386	0.12971476194316744	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	378	0.1270263730946044	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	366	0.12299378982175982	No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA	344	0.11560072048821142	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT	324	0.10887974836680378	No Hit
GTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAA	317	0.1065274081243111	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	312	0.1048471650939592	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC	312	0.1048471650939592	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.002016291636422292	0.0
3	0.0	0.0	0.0	0.003024437454633438	0.0
4	0.0	0.0	0.0	0.00336048606070382	0.0
5	0.0	0.0	0.0	0.004704680484985348	0.0
6	0.0	0.0	0.0	0.0050407290910557305	0.0
7	0.0	0.0	0.0	0.0050407290910557305	0.0
8	0.0	0.0	0.0	0.0050407290910557305	0.0
9	0.0	0.0	0.0	0.00672097212140764	0.0
10	0.0	0.0	0.0	0.008737263757829933	0.0
11	0.0	0.0	0.0	0.010753555394252224	0.0
12	0.0	0.0	0.0	0.0131058956367449	0.0
13	0.0	0.0	0.0	0.015794284485307956	0.0
14	0.0	0.0	0.0	0.019154770546011776	0.0
15	0.0	0.0	0.0	0.025203645455278652	0.0
16	0.0	0.0	0.0	0.03965373551630508	0.0
17	0.0	0.0	0.0	0.059144554668387236	0.0
18	0.0	0.0	0.0	0.06720972121407641	0.0
19	0.0	0.0	0.0	0.08199585988117321	0.0
20	0.0	0.0	0.0	0.08838078339651047	0.0
21	0.0	0.0	0.0	0.12299378982175982	0.0
22	0.0	0.0	0.0	0.16735220582305024	0.0
23	0.0	0.0	0.0	0.22448046885501519	0.0
24	0.0	0.0	0.0	0.32227061322149636	0.0
25	0.0	0.0	0.0	0.34512191843428236	0.0
26	0.0	0.0	0.0	0.3874640427991505	0.0
27	0.0	0.0	0.0	0.43619109067935585	0.0
28	0.0	0.0	0.0	0.48928677043847624	0.0
29	0.0	0.0	0.0	0.5702744845014383	0.0
30	0.0	0.0	0.0	0.7070462671720837	0.0
31	0.0	0.0	0.0	1.085436997607334	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTTAT	30	3.5931263E-4	30.833332	1
TTTACCT	25	0.0054898798	29.6	3
TAAGGAA	25	0.0054898798	29.6	21
GCCGTCT	180	0.0	24.666666	36
CCGTCTT	190	0.0	23.368422	37
AAATAAC	40	0.0019278431	23.125002	33
CTATACC	50	2.6955857E-4	22.2	4
CGTTATA	50	2.6955857E-4	22.2	2
CGTCTTC	80	6.9245107E-7	20.8125	37
CCCCTAT	45	0.0038192468	20.555555	1
ACCTTCT	45	0.0038192468	20.555555	8
TAACTAG	45	0.0038192468	20.555555	26
AGTCGGT	360	0.0	19.01389	11
AAGGGTC	50	0.0070229336	18.5	5
GGCCTAA	50	0.0070229336	18.5	6
CAGTCGG	365	0.0	18.246574	10
ATGCCGT	245	0.0	18.122448	34
GCAGTCG	375	0.0	17.760002	9
GTCGGTG	375	0.0	17.760002	12
GGCAGTC	380	0.0	17.526316	8
>>END_MODULE