FastQCFastQC Report
Fri 10 Feb 2017
ERR1630706.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630706.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1388782
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT187741.351832036993567No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT155371.1187500990076196No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT145601.0484006849167111No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99260.7147270053903348No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA32060.2308497661980066No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC28630.20615186544756486No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27620.19887930575137064No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT27240.19614309517260448No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24910.17936580399227525No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC22910.16496469568297975No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC22320.16071636873173759No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG22170.15963628560854043No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19710.141922922388107No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA19590.14105885588954925No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC19020.13695454002140003No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC18960.1365225067721212No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC18440.13277821861170436No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC17520.12615370878942844No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC17210.12392153700148763No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT16760.12068128763189614No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG16130.11614493851446807No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG15550.11196861710477239No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT15060.10844034556899498No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC14790.1064961959472401No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG14340.10325594657764861No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAAT14130.1017438302051726No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACCGT1150.024.13043617
TACCGTT1150.024.13043618
GTTACCG1251.8189894E-1120.7216
CGAACGA656.903453E-519.92307716
GCGTTAC1303.274181E-1119.92307714
GGTATCA74950.019.277521
TCCGATA701.21942925E-418.58
CCGATAA701.21942925E-418.59
ACCGTTT1409.458745E-1118.519
GGTAAGC500.00703560318.49999833
ATGTACG500.00703560318.49999811
GCGAGTC500.00703560318.49999814
TAACGAA752.0676568E-417.26666613
CGTTTGA1502.5102054E-1017.26666621
AATCGTC752.0676568E-417.26666628
TGCGTTA1502.5102054E-1017.26666613
CGTTACC1554.0199666E-1016.70967915
TCGTGTA1005.879565E-616.6513
GCAGCGT1005.879565E-616.651
TTCTCGA904.4470304E-516.44444523
Produced by FastQC (version 0.11.5)