##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630706.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1388782
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.85128695504406	33.0	31.0	34.0	30.0	34.0
2	31.978808049067457	33.0	31.0	34.0	30.0	34.0
3	32.05044708240746	33.0	31.0	34.0	30.0	34.0
4	35.67482873481943	37.0	35.0	37.0	33.0	37.0
5	35.68263269541224	37.0	35.0	37.0	33.0	37.0
6	35.750750657770624	37.0	35.0	37.0	35.0	37.0
7	35.7217605066886	37.0	35.0	37.0	33.0	37.0
8	35.74080453231681	37.0	35.0	37.0	33.0	37.0
9	37.39638042543754	39.0	37.0	39.0	34.0	39.0
10	37.31237012000444	39.0	37.0	39.0	34.0	39.0
11	37.44846131358269	39.0	37.0	39.0	35.0	39.0
12	37.39027507556982	39.0	37.0	39.0	34.0	39.0
13	37.437427904451525	39.0	37.0	39.0	34.0	39.0
14	38.61217239278735	40.0	38.0	41.0	34.0	41.0
15	38.56231647587598	40.0	38.0	41.0	34.0	41.0
16	38.50582524831111	40.0	38.0	41.0	34.0	41.0
17	38.56032408254139	40.0	38.0	41.0	34.0	41.0
18	38.5831858419824	40.0	38.0	41.0	34.0	41.0
19	38.64290651808563	40.0	38.0	41.0	34.0	41.0
20	38.638101588298234	40.0	38.0	41.0	34.0	41.0
21	38.56482010855555	40.0	38.0	41.0	34.0	41.0
22	38.47141740028312	40.0	38.0	41.0	34.0	41.0
23	38.40127032176396	40.0	38.0	41.0	34.0	41.0
24	38.33817906626094	40.0	38.0	41.0	34.0	41.0
25	38.26630889513257	40.0	38.0	41.0	34.0	41.0
26	38.02095433264544	40.0	37.0	41.0	33.0	41.0
27	37.84690181756388	40.0	37.0	41.0	33.0	41.0
28	37.70261855352388	40.0	37.0	41.0	33.0	41.0
29	37.57142661699245	40.0	36.0	41.0	32.0	41.0
30	37.408404630820385	39.0	36.0	41.0	32.0	41.0
31	37.28728770966214	39.0	36.0	41.0	32.0	41.0
32	37.10229971298591	39.0	35.0	41.0	31.0	41.0
33	36.97504359935541	39.0	35.0	41.0	31.0	41.0
34	36.8324035017735	39.0	35.0	41.0	31.0	41.0
35	36.68365445404678	39.0	35.0	41.0	31.0	41.0
36	36.57365806872497	39.0	35.0	41.0	30.0	41.0
37	36.28732659265457	39.0	35.0	41.0	30.0	41.0
38	36.07779550714223	39.0	35.0	41.0	29.0	41.0
39	35.884843697570965	39.0	35.0	41.0	28.0	41.0
40	35.66359515028277	39.0	35.0	40.0	27.0	41.0
41	35.418242027906466	38.0	35.0	40.0	26.0	41.0
42	35.17016925622596	38.0	35.0	40.0	24.0	41.0
43	34.29881363669748	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	2.0
10	4.0
11	7.0
12	9.0
13	8.0
14	9.0
15	10.0
16	12.0
17	23.0
18	70.0
19	145.0
20	324.0
21	652.0
22	1124.0
23	1964.0
24	3243.0
25	4969.0
26	7595.0
27	10996.0
28	15402.0
29	20964.0
30	27217.0
31	34573.0
32	42587.0
33	54022.0
34	69541.0
35	89380.0
36	124041.0
37	196627.0
38	301908.0
39	381352.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.443057297689634	20.430204308523585	12.710562204867287	21.4161761889195
2	19.47267461703853	22.268073750955875	33.47652835362209	24.782723278383504
3	22.709611731718873	25.467207956324316	28.563662259447486	23.25951805250932
4	13.825207988006758	16.28232508773875	33.85333335253481	36.03913357171968
5	12.970574215391617	40.066619527038796	32.39961347425298	14.563192783316603
6	32.53743208077294	33.65431003570035	16.782547584862133	17.025710298664585
7	27.22414317005837	32.99452325850997	21.178485896274577	18.60284767515708
8	29.729287966001866	30.356600243954773	20.122308612870846	19.791803177172515
9	27.798243352808434	12.874518822968618	22.16388173233812	37.16335609188483
10	20.031221602814554	27.468025939276288	30.55216729479501	21.948585163114153
11	33.123557188961264	23.10787438201244	20.394345548833435	23.37422288019286
12	22.116142058292805	28.932186621082355	27.424750608806853	21.526920711817983
13	29.638632989194846	20.565718737714054	27.731206193628662	22.064442079462435
14	22.84527017199244	22.598651192195753	27.562425204243716	26.99365343156809
15	26.795494181232186	26.17214220806433	25.986871949665247	21.045491661038234
16	24.040202133956228	25.921130890233314	27.44858444305874	22.590082532751723
17	22.363481093504955	27.021447570605034	27.11303861945215	23.502032716437856
18	21.49034189671237	23.557476983428643	30.91010684182255	24.042074278036438
19	21.964498387795924	25.485785386043307	31.009042455907405	21.540673770253356
20	23.206665985014208	23.475174649441023	31.838834316689013	21.47932504885576
21	23.89381486799224	24.818294015907462	30.329382149250208	20.95850896685009
22	24.50175765526915	24.229792724848103	30.15916104903433	21.10928857084841
23	22.791482032457218	24.379348234640137	30.667016133561635	22.162153599341007
24	21.325737228737125	26.05362108667883	30.32578187217288	22.294859812411165
25	21.94613697470157	25.517755846489948	30.777328623210842	21.758778555597637
26	21.886156358593357	26.494943050817195	30.066993955854844	21.55190663473461
27	22.662016068756653	25.426956858599837	30.337662786528053	21.57336428611546
28	21.04757982174308	25.77402356885386	31.02308353650897	22.15531307289409
29	21.070693600579503	25.86266239049757	31.500264260337474	21.56637974858545
30	19.92890172827701	27.381763300503607	31.459797145988354	21.22953782523103
31	21.81803911629039	27.285347880372875	29.43276914591347	21.463843857423267
32	20.592144771461612	26.620880742981978	30.33888688073434	22.448087604822067
33	20.090266146882666	26.36029268812528	30.756086988454634	22.793354176537424
34	21.37462899144718	26.077670937555354	30.84702998742783	21.70067008356963
35	20.482984370477155	26.76914015302618	30.898081916384285	21.849793560112385
36	20.468439251084764	27.886666157827506	29.67816403150386	21.966730559583866
37	21.32379307911537	26.40111983018213	30.2886270127349	21.9864600779676
38	20.5862403170548	25.966854409115324	30.202796407211498	23.244108866618372
39	20.909977231847762	26.840785666864917	30.367185058562107	21.88205204272521
40	20.028125364528055	26.55571572788242	30.489810495815757	22.92634841177377
41	19.899595472867592	26.78577343312341	30.74283796881008	22.571793125198916
42	20.12648493428054	26.999773902599543	30.968863363724473	21.904877799395443
43	19.481963331898022	25.801097652475335	30.265225211732293	24.45171380389435
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	797.0
1	740.5
2	684.0
3	1788.0
4	2892.0
5	2892.0
6	4152.0
7	5412.0
8	5319.5
9	5227.0
10	7630.0
11	10033.0
12	10033.0
13	17481.5
14	24930.0
15	31557.5
16	38185.0
17	35497.5
18	32810.0
19	32810.0
20	34738.5
21	36667.0
22	27569.0
23	18471.0
24	18178.5
25	17886.0
26	17886.0
27	19717.0
28	21548.0
29	24443.0
30	27338.0
31	31664.0
32	35990.0
33	35990.0
34	42003.0
35	48016.0
36	52321.5
37	56627.0
38	63345.5
39	70064.0
40	70064.0
41	74471.0
42	78878.0
43	82636.0
44	86394.0
45	85797.0
46	85200.0
47	85200.0
48	90300.0
49	95400.0
50	93190.5
51	90981.0
52	92314.5
53	93648.0
54	93648.0
55	87587.0
56	81526.0
57	75443.0
58	69360.0
59	65741.5
60	62123.0
61	62123.0
62	59470.5
63	56818.0
64	49879.5
65	42941.0
66	39516.0
67	36091.0
68	36091.0
69	29199.5
70	22308.0
71	19042.5
72	15777.0
73	11355.5
74	6934.0
75	6934.0
76	5238.5
77	3543.0
78	2936.5
79	2330.0
80	1881.0
81	1432.0
82	1432.0
83	1300.5
84	1169.0
85	1112.5
86	1056.0
87	870.5
88	685.0
89	685.0
90	600.5
91	516.0
92	292.5
93	69.0
94	43.0
95	17.0
96	17.0
97	13.0
98	9.0
99	4.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1388782.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.44819942946633
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.85924471565366	37.27392433125794
2	9.48115426002374	8.42840470742139
3	2.394487728610205	3.192920042780287
4	0.9941359431929389	1.7675021065216143
5	0.5370451090448873	1.193534405472332
6	0.3659725362728371	0.9760092166777596
7	0.28313192901664613	0.8809293112046973
8	0.2044350363536846	0.7269415412975018
9	0.16779224843210577	0.6712256988925916
>10	1.3437332209724757	12.319599668870977
>50	0.19576895792954405	6.091192011226119
>100	0.15081028246847883	12.963005769758794
>500	0.015455204691536956	4.726691286986511
>1k	0.0061820818766147815	4.536440624928844
>5k	1.626863651740732E-4	0.7177605745284721
>10k+	4.880590955222196E-4	3.5339187021741854
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	18774	1.351832036993567	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	15537	1.1187500990076196	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	14560	1.0484006849167111	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9926	0.7147270053903348	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	3206	0.2308497661980066	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	2863	0.20615186544756486	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2762	0.19887930575137064	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2724	0.19614309517260448	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2491	0.17936580399227525	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	2291	0.16496469568297975	No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC	2232	0.16071636873173759	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	2217	0.15963628560854043	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1971	0.141922922388107	No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA	1959	0.14105885588954925	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	1902	0.13695454002140003	No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC	1896	0.1365225067721212	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1844	0.13277821861170436	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	1752	0.12615370878942844	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	1721	0.12392153700148763	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	1676	0.12068128763189614	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG	1613	0.11614493851446807	No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG	1555	0.11196861710477239	No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT	1506	0.10844034556899498	No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC	1479	0.1064961959472401	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	1434	0.10325594657764861	No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAAT	1413	0.1017438302051726	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	4.320332492788645E-4	0.0
3	0.0	0.0	0.0	0.0010080775816506838	0.0
4	0.0	0.0	0.0	0.001872144080208413	0.0
5	0.0	0.0	0.0	0.0020161551633013676	0.0
6	0.0	0.0	0.0	0.002088160704847845	0.0
7	0.0	0.0	0.0	0.002736210578766142	0.0
8	0.0	0.0	0.0	0.0030242327449520514	0.0
9	0.0	0.0	0.0	0.0048243712836139865	0.0
10	0.0	0.0	0.0	0.006840526446915355	0.0
11	0.0	0.0	0.0	0.007632587403926606	0.0
12	0.0	0.0	0.0	0.009000692693309676	0.0
13	0.0	0.0	0.0	0.009648742567227973	0.0
14	0.0	0.0	0.0	0.011736903272075818	0.0
15	0.0	0.0	0.0	0.01476113601702787	0.0
16	0.0	0.0	0.0	0.021313640297757317	0.0
17	0.0	0.0	0.0	0.03074636624034586	0.0
18	0.0	0.0	0.0	0.03470667102540211	0.0
19	0.0	0.0	0.0	0.041115164223038604	0.0
20	0.0	0.0	0.0	0.04514747454964134	0.0
21	0.0	0.0	0.0	0.05861251081883262	0.0
22	0.0	0.0	0.0	0.08014216774122937	0.0
23	0.0	0.0	0.0	0.10880037327672738	0.0
24	0.0	0.0	0.0	0.1508516095398702	0.0
25	0.0	0.0	0.0	0.16194046293802772	0.0
26	0.0	0.0	0.0	0.18130995361403013	0.0
27	0.0	0.0	0.0	0.20550381557364655	0.0
28	0.0	0.0	0.0	0.24834711279380062	0.0
29	0.0	0.0	0.0	0.3112079505638754	0.0
30	0.0	0.0	0.0	0.42029634600678867	0.0
31	0.0	0.0	0.0	0.7536099978254327	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACCGT	115	0.0	24.130436	17
TACCGTT	115	0.0	24.130436	18
GTTACCG	125	1.8189894E-11	20.72	16
CGAACGA	65	6.903453E-5	19.923077	16
GCGTTAC	130	3.274181E-11	19.923077	14
GGTATCA	7495	0.0	19.27752	1
TCCGATA	70	1.21942925E-4	18.5	8
CCGATAA	70	1.21942925E-4	18.5	9
ACCGTTT	140	9.458745E-11	18.5	19
GGTAAGC	50	0.007035603	18.499998	33
ATGTACG	50	0.007035603	18.499998	11
GCGAGTC	50	0.007035603	18.499998	14
TAACGAA	75	2.0676568E-4	17.266666	13
CGTTTGA	150	2.5102054E-10	17.266666	21
AATCGTC	75	2.0676568E-4	17.266666	28
TGCGTTA	150	2.5102054E-10	17.266666	13
CGTTACC	155	4.0199666E-10	16.709679	15
TCGTGTA	100	5.879565E-6	16.65	13
GCAGCGT	100	5.879565E-6	16.65	1
TTCTCGA	90	4.4470304E-5	16.444445	23
>>END_MODULE