FastQCFastQC Report
Fri 10 Feb 2017
ERR1630704.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630704.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences986044
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT51150.5187395288648377No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT43880.4450105674797473No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT37570.3810174799501848No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28370.2877153555013772No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAATAC555.1423814E-420.1818183
GTATTAC759.263582E-619.7333341
GGTATCA21450.018.8881131
TAATAGT902.1515843E-618.54
AACCGGC904.4449247E-516.44444516
GTATTAG2300.016.0869561
CGAACTA700.00259214315.85714324
CTATACA700.00259214315.8571434
ATTCGGA700.00259214315.85714311
GCAGTCG1059.340323E-615.8571429
AGTCGGT1059.340323E-615.85714211
TATACTA1302.587676E-715.6538485
GAACCGG957.0580485E-515.57894815
ATACTGA1508.095776E-814.86
GTAGACC750.00410385614.83
TAGTTCG908.272543E-414.38888812
GTGTAAC908.272543E-414.3888881
GTAACAC908.272543E-414.3888883
TGAACCG1051.655254E-414.09523814
ACGGTAT1051.655254E-414.0952389
Produced by FastQC (version 0.11.5)