FastQCFastQC Report
Fri 10 Feb 2017
ERR1630703.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630703.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1128
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT161.4184397163120568TruSeq Adapter, Index 1 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGT141.2411347517730498No Hit
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC110.975177304964539No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC80.7092198581560284No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT70.6205673758865249No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT50.44326241134751776No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTC50.44326241134751776No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATG40.3546099290780142No Hit
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTC40.3546099290780142RNA PCR Primer, Index 1 (95% over 22bp)
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG30.26595744680851063No Hit
GAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGC30.26595744680851063No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTT30.26595744680851063No Hit
TCACTGTACCGGCCGTGCGTACTTAGACATGCATGGCTTAATC20.1773049645390071No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTC20.1773049645390071No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.1773049645390071No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT20.1773049645390071No Hit
CACCAGGAGTGGAGCCTGCGGCTTAATTTGACCCAACACGGGA20.1773049645390071No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCG20.1773049645390071No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC20.1773049645390071No Hit
GGACTTAATCAACGCAAGCTTATGACTGTCTCTTATACACATC20.1773049645390071No Hit
GGCCGGAGTCGTTGGCTGTGAATACCACCTCTGCATGCTCATG20.1773049645390071No Hit
GTCCAGCGGGCTGAGCTCCCTGGGGACAGCAGCATGCGGGGAC20.1773049645390071No Hit
GAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATG20.1773049645390071No Hit
GTATAAGAGACAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.1773049645390071No Hit
TATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTAT20.1773049645390071No Hit
ACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCA20.1773049645390071No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGT20.1773049645390071No Hit
GCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGAGCGAGC20.1773049645390071No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)