##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630703.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1128
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.749113475177303	31.0	30.0	31.0	26.0	34.0
2	30.041666666666668	31.0	30.0	31.0	26.0	34.0
3	29.88918439716312	31.0	30.0	31.0	26.0	34.0
4	33.73492907801418	35.0	33.0	37.0	28.0	37.0
5	33.472517730496456	35.0	33.0	37.0	28.0	37.0
6	33.422872340425535	35.0	33.0	37.0	28.0	37.0
7	33.35017730496454	35.0	33.0	36.0	28.0	37.0
8	33.39716312056738	35.0	33.0	37.0	28.0	37.0
9	34.72429078014184	37.0	33.0	39.0	28.0	39.0
10	34.416666666666664	37.0	32.0	39.0	27.0	39.0
11	34.741134751773046	37.0	33.0	39.0	29.0	39.0
12	34.50797872340426	37.0	33.0	39.0	27.0	39.0
13	34.6072695035461	37.0	33.0	39.0	27.0	39.0
14	35.32890070921986	37.0	33.0	39.0	29.0	40.0
15	35.21719858156028	37.0	33.0	39.0	27.0	41.0
16	35.42553191489362	37.0	33.0	39.0	27.0	40.0
17	35.001773049645394	37.0	33.0	39.0	27.0	40.0
18	35.001773049645394	37.0	33.0	39.0	27.0	40.0
19	35.17464539007092	37.0	33.0	39.0	27.0	40.0
20	35.093971631205676	37.0	33.0	39.0	27.0	40.0
21	34.95744680851064	37.0	33.0	39.0	27.0	40.0
22	34.9104609929078	37.0	33.0	39.0	27.0	41.0
23	34.830673758865245	37.0	32.0	39.0	26.0	40.0
24	34.491134751773046	37.0	32.0	39.0	25.0	40.0
25	34.1072695035461	36.0	32.0	39.0	25.0	40.0
26	33.5354609929078	36.0	31.0	39.0	24.0	40.0
27	33.2508865248227	36.0	31.0	39.0	23.0	40.0
28	33.326241134751776	36.0	31.0	39.0	23.0	40.0
29	33.156028368794324	36.0	31.0	39.0	22.0	40.0
30	32.87056737588652	35.0	30.0	38.0	22.0	40.0
31	32.780141843971634	35.0	30.0	38.0	21.0	40.0
32	32.587765957446805	35.0	30.0	38.0	19.0	40.0
33	32.541666666666664	35.0	30.0	39.0	19.0	40.0
34	32.22517730496454	35.0	30.0	38.0	19.0	40.0
35	32.551418439716315	35.0	30.0	39.0	19.0	40.0
36	32.408687943262414	35.0	30.0	38.0	19.0	40.0
37	32.001773049645394	35.0	30.0	38.0	18.0	40.0
38	31.967198581560282	35.0	30.0	38.0	15.0	40.0
39	31.671985815602838	35.0	28.0	38.0	17.0	40.0
40	31.43705673758865	35.0	29.0	38.0	15.0	40.0
41	31.50709219858156	34.0	27.0	38.0	17.0	40.0
42	31.620567375886523	34.0	29.0	38.0	17.0	40.0
43	30.545212765957448	33.0	27.0	38.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	2.0
20	6.0
21	5.0
22	7.0
23	9.0
24	14.0
25	21.0
26	38.0
27	30.0
28	43.0
29	57.0
30	55.0
31	81.0
32	93.0
33	99.0
34	113.0
35	113.0
36	116.0
37	97.0
38	82.0
39	45.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.184397163120565	20.47872340425532	14.184397163120568	26.152482269503547
2	17.109929078014186	22.340425531914892	34.13120567375886	26.418439716312058
3	20.124113475177303	22.163120567375884	32.62411347517731	25.088652482269502
4	14.095744680851062	17.02127659574468	31.648936170212767	37.234042553191486
5	13.741134751773048	34.04255319148936	36.08156028368794	16.134751773049647
6	33.244680851063826	35.37234042553192	15.24822695035461	16.134751773049647
7	25.44326241134752	31.73758865248227	20.212765957446805	22.606382978723406
8	30.0531914893617	32.71276595744681	20.212765957446805	17.02127659574468
9	25.44326241134752	12.145390070921986	20.56737588652482	41.843971631205676
10	18.97163120567376	28.102836879432623	29.432624113475175	23.49290780141844
11	35.726950354609926	19.9468085106383	20.124113475177303	24.20212765957447
12	19.680851063829788	27.925531914893615	26.24113475177305	26.152482269503547
13	28.54609929078014	19.414893617021274	27.393617021276594	24.645390070921984
14	25.0	20.74468085106383	21.71985815602837	32.5354609929078
15	24.73404255319149	27.30496453900709	22.78368794326241	25.177304964539005
16	27.30496453900709	22.96099290780142	24.73404255319149	25.0
17	21.71985815602837	30.141843971631204	24.02482269503546	24.113475177304963
18	26.063829787234045	23.847517730496453	24.379432624113477	25.70921985815603
19	23.31560283687943	25.79787234042553	28.98936170212766	21.897163120567377
20	24.911347517730498	25.0	26.418439716312058	23.670212765957448
21	27.570921985815605	25.53191489361702	24.46808510638298	22.429078014184398
22	25.177304964539005	24.73404255319149	26.50709219858156	23.581560283687946
23	22.340425531914892	23.93617021276596	25.79787234042553	27.925531914893615
24	23.404255319148938	27.393617021276594	26.152482269503547	23.04964539007092
25	22.69503546099291	28.368794326241137	26.595744680851062	22.340425531914892
26	26.152482269503547	26.50709219858156	26.418439716312058	20.921985815602838
27	23.93617021276596	26.684397163120565	27.482269503546096	21.897163120567377
28	24.46808510638298	21.099290780141843	30.141843971631204	24.29078014184397
29	23.581560283687946	27.74822695035461	27.127659574468083	21.54255319148936
30	23.670212765957448	24.113475177304963	30.0531914893617	22.163120567375884
31	23.93617021276596	24.55673758865248	26.684397163120565	24.822695035460992
32	25.620567375886527	20.921985815602838	27.393617021276594	26.063829787234045
33	21.45390070921986	24.822695035460992	29.25531914893617	24.46808510638298
34	25.44326241134752	24.73404255319149	26.95035460992908	22.872340425531913
35	22.78368794326241	22.163120567375884	32.358156028368796	22.69503546099291
36	25.354609929078016	23.404255319148938	26.152482269503547	25.088652482269502
37	22.872340425531913	25.79787234042553	26.861702127659576	24.46808510638298
38	24.55673758865248	23.75886524822695	28.191489361702125	23.49290780141844
39	24.73404255319149	20.74468085106383	29.78723404255319	24.73404255319149
40	22.340425531914892	22.78368794326241	30.76241134751773	24.113475177304963
41	22.25177304964539	22.69503546099291	27.482269503546096	27.570921985815605
42	23.04964539007092	22.340425531914892	31.29432624113475	23.31560283687943
43	22.69503546099291	20.74468085106383	28.900709219858157	27.659574468085108
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	1.0
2	1.0
3	1.5
4	2.0
5	2.0
6	1.5
7	1.0
8	1.0
9	1.0
10	4.0
11	7.0
12	7.0
13	9.0
14	11.0
15	10.5
16	10.0
17	12.5
18	15.0
19	15.0
20	17.0
21	19.0
22	18.5
23	18.0
24	15.5
25	13.0
26	13.0
27	16.0
28	19.0
29	19.5
30	20.0
31	20.0
32	20.0
33	20.0
34	24.0
35	28.0
36	28.5
37	29.0
38	40.0
39	51.0
40	51.0
41	46.5
42	42.0
43	46.5
44	51.0
45	64.0
46	77.0
47	77.0
48	82.5
49	88.0
50	99.0
51	110.0
52	91.0
53	72.0
54	72.0
55	86.0
56	100.0
57	93.5
58	87.0
59	74.5
60	62.0
61	62.0
62	52.5
63	43.0
64	41.0
65	39.0
66	36.0
67	33.0
68	33.0
69	30.0
70	27.0
71	21.0
72	15.0
73	10.5
74	6.0
75	6.0
76	6.5
77	7.0
78	5.0
79	3.0
80	1.5
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1128.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	92.2872340425532
#Duplication Level	Percentage of deduplicated	Percentage of total
1	97.31027857829011	89.80496453900709
2	1.536983669548511	2.8368794326241136
3	0.2881844380403458	0.7978723404255319
4	0.19212295869356388	0.7092198581560284
5	0.19212295869356388	0.8865248226950355
6	0.0	0.0
7	0.09606147934678194	0.6205673758865249
8	0.09606147934678194	0.7092198581560284
9	0.0	0.0
>10	0.2881844380403458	3.6347517730496453
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT	16	1.4184397163120568	TruSeq Adapter, Index 1 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGT	14	1.2411347517730498	No Hit
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC	11	0.975177304964539	No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC	8	0.7092198581560284	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7	0.6205673758865249	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5	0.44326241134751776	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTC	5	0.44326241134751776	No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATG	4	0.3546099290780142	No Hit
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTC	4	0.3546099290780142	RNA PCR Primer, Index 1 (95% over 22bp)
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG	3	0.26595744680851063	No Hit
GAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGC	3	0.26595744680851063	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTT	3	0.26595744680851063	No Hit
TCACTGTACCGGCCGTGCGTACTTAGACATGCATGGCTTAATC	2	0.1773049645390071	No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTC	2	0.1773049645390071	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2	0.1773049645390071	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2	0.1773049645390071	No Hit
CACCAGGAGTGGAGCCTGCGGCTTAATTTGACCCAACACGGGA	2	0.1773049645390071	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCG	2	0.1773049645390071	No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC	2	0.1773049645390071	No Hit
GGACTTAATCAACGCAAGCTTATGACTGTCTCTTATACACATC	2	0.1773049645390071	No Hit
GGCCGGAGTCGTTGGCTGTGAATACCACCTCTGCATGCTCATG	2	0.1773049645390071	No Hit
GTCCAGCGGGCTGAGCTCCCTGGGGACAGCAGCATGCGGGGAC	2	0.1773049645390071	No Hit
GAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATG	2	0.1773049645390071	No Hit
GTATAAGAGACAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2	0.1773049645390071	No Hit
TATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTAT	2	0.1773049645390071	No Hit
ACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCA	2	0.1773049645390071	No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGT	2	0.1773049645390071	No Hit
GCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGAGCGAGC	2	0.1773049645390071	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.08865248226950355	0.0
23	0.0	0.0	0.0	0.08865248226950355	0.0
24	0.0	0.0	0.0	0.1773049645390071	0.0
25	0.0	0.0	0.0	0.26595744680851063	0.0
26	0.0	0.0	0.0	0.5319148936170213	0.0
27	0.0	0.0	0.0	0.6205673758865248	0.0
28	0.0	0.0	0.0	0.7092198581560284	0.0
29	0.0	0.0	0.0	0.7978723404255319	0.0
30	0.0	0.0	0.0	0.8865248226950354	0.0
31	0.0	0.0	0.0	1.1524822695035462	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE