FastQCFastQC Report
Fri 10 Feb 2017
ERR1630702.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630702.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1133989
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT49650.437834934906776No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT41390.36499472217102635No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT36910.32548816611095877No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28510.251413373498332No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTGA2300.018.513
CATTACG500.00703482418.529
TTAACGG500.00703482418.535
TATACTG701.21917896E-418.55
CTAGCGG1103.851528E-818.529
TCTAGAC3350.018.223883
GGTATCA26800.018.223881
CTAGACA3550.017.7183114
TAGACAG953.6062993E-617.5263165
CGCAGTA852.7230639E-517.4117642
GGCCGCT2450.017.36734611
ACTATAG752.067235E-417.2666663
CTCTAGT2900.017.22413827
GTCTAGA2600.017.0769231
TAAGCTA1005.8776477E-616.65000233
GTATTAT803.3825164E-416.18751
CCTATGC700.002592464715.8571438
AAGACGG2100.015.8571425
GACGGAC2100.015.8571427
CGAACTA957.05954E-515.57894824
Produced by FastQC (version 0.11.5)