FastQCFastQC Report
Fri 10 Feb 2017
ERR1630700.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630700.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1475534
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT131680.8924226754517348No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT105070.7120811855233427No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT95230.645393464332235No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76500.5184563690162342No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22070.149572968159324No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC21960.14882747534113075No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT21830.14794643837417504No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20430.13845834796080606No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT17990.12192196181179153No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA17920.12144755729112308No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA17040.11548361474557685No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG16000.10843531900993132No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15550.105385575662777No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT1708.54925E-1116.32352836
AGCACCG803.3833797E-416.18755
ATACCGT1403.4748155E-815.8571436
TAGTACG750.0041051414.84
CTAATAC2151.2732926E-1114.6279073
GGTATCA68050.014.5716391
ATAGTAC1405.997663E-714.5357153
GTACAAT1152.2105745E-514.478261
GTACTAT1152.2105745E-514.478261
ATAGACG800.006299739213.8753
GTATACG800.006299739213.8751
CTCGTAT2009.840733E-1013.87499929
ACGCTTC1203.3016066E-513.87499931
ATGCCGT2009.840733E-1013.87499934
GCTTTAT6300.013.8015871
TAACCCG2551.8189894E-1213.78431328
TCGCGTA950.001245366313.6315799
GTAGTAC950.001245366313.6315793
GTATAGG1805.160655E-813.3611111
TATACTG1805.160655E-813.3611115
Produced by FastQC (version 0.11.5)