FastQCFastQC Report
Fri 10 Feb 2017
ERR1630699.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630699.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences630729
Sequences flagged as poor quality0
Sequence length43
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT240143.807340395003242No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT205313.2551222474311468No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT163672.594933798826437No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85981.3631845055483416No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT23500.3725847392461739No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGT21420.3396070261554487No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20210.3204228757517095No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA15940.252723435897192No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG15090.2392469666053091No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14430.2287828845669059No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13760.2181602558309512No Hit
TCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT13520.21435513508971366Illumina PCR Primer Index 10 (95% over 22bp)
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA11720.18581672953043224No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT11380.1804261418136791No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG9760.15474157681032583No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATG9510.15077790937153673No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9470.1501437225813305No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA9300.1474484287229539No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9040.14332621458661327No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA8720.13825272026496324No Hit
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCC7580.12017839674408502No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7450.1181172896759147No Hit
GAACAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAA6770.1073361142424084No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCTTT250.00549448629.627
GTACTAG250.00549448629.61
TTAGACC405.9359452E-527.754
GTTAGAC358.864938E-426.4285723
GTCCTAA400.001930226523.1251
CAGTCGG5400.021.58333410
AGTCGGT5400.021.58333411
CGCCTTA4600.020.5108725
GCAGTCG5700.020.4473699
CCGTCTT2100.020.26190637
GAAACCG555.139559E-420.1818185
GGAACGC555.139559E-420.1818188
GGCAGTC6000.020.0416668
CTCGCCT4550.019.92307723
GATTCCT5500.019.84545318
CTTATTG5400.019.52777928
TCGCCTT4750.019.47368424
GCCGTCT2200.019.34090836
TCGGTGA5750.019.30434813
CGGTGAT5850.019.29059814
Produced by FastQC (version 0.11.5)