##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630699.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	630729
Sequences flagged as poor quality	0
Sequence length	43
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.96629455756751	33.0	31.0	34.0	30.0	34.0
2	32.00981404057844	33.0	31.0	34.0	30.0	34.0
3	32.01963600849176	33.0	31.0	34.0	30.0	34.0
4	35.702182712385195	37.0	35.0	37.0	33.0	37.0
5	35.73371447959425	37.0	35.0	37.0	35.0	37.0
6	35.8954416238987	37.0	35.0	37.0	35.0	37.0
7	35.816317309018615	37.0	35.0	37.0	35.0	37.0
8	35.8760862430616	37.0	35.0	37.0	35.0	37.0
9	37.469337861427015	39.0	37.0	39.0	35.0	39.0
10	37.40839726728912	39.0	37.0	39.0	34.0	39.0
11	37.57336193515757	39.0	37.0	39.0	35.0	39.0
12	37.52825539970415	39.0	37.0	39.0	35.0	39.0
13	37.5885253413114	39.0	37.0	39.0	35.0	39.0
14	38.59454060301651	40.0	38.0	41.0	34.0	41.0
15	38.57483324851085	40.0	38.0	41.0	34.0	41.0
16	38.46791886848393	40.0	38.0	41.0	34.0	41.0
17	38.671763942992946	40.0	38.0	41.0	35.0	41.0
18	38.80491938693163	40.0	38.0	41.0	35.0	41.0
19	38.95190961569866	40.0	38.0	41.0	35.0	41.0
20	38.960693102743015	40.0	39.0	41.0	35.0	41.0
21	38.9187590867076	40.0	38.0	41.0	35.0	41.0
22	38.770839774292924	40.0	38.0	41.0	35.0	41.0
23	38.68493441715856	40.0	38.0	41.0	35.0	41.0
24	38.581806132269165	40.0	38.0	41.0	35.0	41.0
25	38.50303854745857	40.0	38.0	41.0	34.0	41.0
26	38.22696752487994	40.0	37.0	41.0	34.0	41.0
27	37.991812648538435	40.0	36.0	41.0	34.0	41.0
28	37.86221974889374	40.0	36.0	41.0	33.0	41.0
29	37.65588549123316	39.0	36.0	41.0	33.0	41.0
30	37.3874310520049	39.0	35.0	41.0	33.0	41.0
31	37.0448163949969	39.0	35.0	41.0	33.0	41.0
32	36.708446892405455	38.0	35.0	41.0	32.0	41.0
33	36.406307621815394	38.0	35.0	41.0	31.0	41.0
34	36.154023360270415	38.0	35.0	41.0	31.0	41.0
35	35.886226889837	38.0	35.0	41.0	30.0	41.0
36	35.60290711224631	38.0	35.0	41.0	30.0	41.0
37	35.203656403938936	38.0	35.0	40.0	26.0	41.0
38	34.81937726028136	38.0	35.0	40.0	24.0	41.0
39	34.48049796346767	37.0	35.0	40.0	21.0	41.0
40	34.00805734316957	37.0	34.0	40.0	18.0	41.0
41	33.5980571687682	37.0	33.0	40.0	15.0	41.0
42	33.12731775453483	36.0	33.0	40.0	10.0	41.0
43	32.31575209004184	35.0	33.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	8.0
11	16.0
12	14.0
13	10.0
14	5.0
15	8.0
16	11.0
17	14.0
18	38.0
19	64.0
20	131.0
21	262.0
22	538.0
23	930.0
24	1534.0
25	2498.0
26	3825.0
27	6082.0
28	8616.0
29	11777.0
30	15035.0
31	17840.0
32	21185.0
33	25892.0
34	32641.0
35	40850.0
36	57402.0
37	109156.0
38	123194.0
39	151150.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	52.118580246032764	18.818066079092606	14.62085935481007	14.442494320064561
2	17.29538359580739	25.61290189605996	35.675385149565024	21.416329358567626
3	23.58477254098036	29.95406902171931	30.34108151044268	16.120076926857653
4	11.321344032064484	16.745068008605916	31.08387278847175	40.84971517085785
5	10.189479158243874	46.160236805347466	29.184007711711367	14.466276324697294
6	29.079683984722436	32.08398535662702	19.5277210973334	19.30860956131714
7	24.299659600240357	35.90686332799031	20.170627955904994	19.62284911586434
8	35.278384218895916	28.465791171802785	19.703866478313188	16.55195813098811
9	29.318296764537543	9.223137036667096	23.039688994798084	38.41887720399728
10	19.522806149709304	32.76034556838198	27.727756294700264	19.98909198720845
11	29.76016641061375	24.469146019922977	18.43406597762272	27.336621591840554
12	20.775483607064206	34.508481455585525	29.222534559216403	15.493500378133874
13	33.41942419010383	20.22310691279456	28.78272602020836	17.574742876893247
14	23.690998828339904	22.752085285439545	34.098479695717174	19.45843619050337
15	27.592833055083883	24.894685356151374	29.507601521414113	18.004880067350637
16	19.994799668320308	24.91656480041349	32.175149707719164	22.913485823547038
17	16.26578134190754	29.889381969118272	31.925755752470554	21.919080936503633
18	16.54957993052484	22.950268657379	37.34821135543157	23.15194005666459
19	18.698838962533827	25.990877223022885	39.3032506829399	16.007033131503388
20	21.45549039286286	22.179890253975955	40.619346819315425	15.745272533845755
21	23.94911285195385	22.39820905650446	36.11392531499265	17.538752776549043
22	20.896930377388703	24.273816488539453	37.558602823082495	17.270650310989346
23	19.29909675946405	24.914979333437977	36.383454700830306	19.402469206267668
24	17.099895517726313	26.677542970118708	38.55617864407693	17.66638286807805
25	17.303628024080073	26.96292702571152	37.179200575841605	18.554244374366803
26	18.713900898801228	26.470005343023708	37.02905685326027	17.78703690491479
27	16.429718627175856	28.116037157004037	37.64849880059423	17.805745415225875
28	16.267366808883054	27.454263241423817	38.328505586392886	17.949864363300243
29	15.275498669000473	28.476889440631396	38.90101771125158	17.346594179116543
30	15.907465805441007	29.90127297143464	37.841291584816936	16.349969638307417
31	15.8410347391669	30.57049858179979	36.49744977636988	17.091016902663426
32	14.068482660540422	31.437907564104396	38.11050387725949	16.383105898095696
33	14.888327633579557	30.356936180197835	38.25462282533386	16.50011336088875
34	15.224446632388872	30.84177198131052	36.6862788931538	17.24750249314682
35	14.307095440355525	31.454079327254654	36.33715906514525	17.90166616724457
36	14.631799076941126	32.24221496078347	35.37874427844605	17.747241683829344
37	14.189136697377162	32.887341473120784	35.15424215471304	17.769279674789015
38	14.71123097241446	31.638944776599775	35.420442059902115	18.22938219108365
39	14.76592958306975	31.907522882252128	35.62465020634853	17.7018973283296
40	14.076885635510655	31.68333785191421	36.20588239957256	18.033894113002574
41	13.71714317876616	32.76494342261098	35.005842445804774	18.51207095281809
42	13.745840131022991	32.316731908632704	35.63511428838693	18.30231367195737
43	13.373413938474368	32.93807641633729	34.69017597097962	18.998333674208734
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	29.0
1	128.0
2	227.0
3	1190.5
4	2154.0
5	2154.0
6	3346.0
7	4538.0
8	4858.0
9	5178.0
10	7148.5
11	9119.0
12	9119.0
13	15594.5
14	22070.0
15	34339.5
16	46609.0
17	40859.0
18	35109.0
19	35109.0
20	37058.0
21	39007.0
22	27650.5
23	16294.0
24	15231.5
25	14169.0
26	14169.0
27	14751.5
28	15334.0
29	16145.5
30	16957.0
31	17334.5
32	17712.0
33	17712.0
34	17822.5
35	17933.0
36	18538.0
37	19143.0
38	21033.0
39	22923.0
40	22923.0
41	24112.5
42	25302.0
43	26742.5
44	28183.0
45	31199.5
46	34216.0
47	34216.0
48	36219.0
49	38222.0
50	37450.5
51	36679.0
52	34947.5
53	33216.0
54	33216.0
55	34164.5
56	35113.0
57	31652.5
58	28192.0
59	26081.0
60	23970.0
61	23970.0
62	19848.0
63	15726.0
64	12540.5
65	9355.0
66	7745.5
67	6136.0
68	6136.0
69	5173.5
70	4211.0
71	3695.5
72	3180.0
73	2631.0
74	2082.0
75	2082.0
76	1630.0
77	1178.0
78	927.0
79	676.0
80	509.0
81	342.0
82	342.0
83	245.5
84	149.0
85	107.0
86	65.0
87	43.5
88	22.0
89	22.0
90	14.0
91	6.0
92	4.0
93	2.0
94	1.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	630729.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.010050295388744
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.1134141710799	46.2708314406797
2	10.001626657457443	11.003799709248696
3	2.6147372637389372	4.315104851625183
4	1.0397472809266282	2.2878620087307007
5	0.5484205183457225	1.5084320148610681
6	0.309279418300999	1.0208085813639918
7	0.2214535174917095	0.8527518394716783
8	0.1575504518823068	0.6933486625685538
9	0.1254032204450544	0.6208593717497548
>10	0.7349914718093141	7.634241101858562
>50	0.07534879838300489	2.905587829459238
>100	0.04994383489578672	5.622826593046513
>500	0.004041697621624265	1.6682989707945806
>1k	0.0028869268725887614	2.556364448632115
>5k	2.886926872588761E-4	1.3654483151729466
>10k+	8.660780617766285E-4	9.673434260736743
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	24014	3.807340395003242	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	20531	3.2551222474311468	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	16367	2.594933798826437	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8598	1.3631845055483416	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2350	0.3725847392461739	No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGT	2142	0.3396070261554487	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2021	0.3204228757517095	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	1594	0.252723435897192	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	1509	0.2392469666053091	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1443	0.2287828845669059	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1376	0.2181602558309512	No Hit
TCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT	1352	0.21435513508971366	Illumina PCR Primer Index 10 (95% over 22bp)
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	1172	0.18581672953043224	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	1138	0.1804261418136791	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	976	0.15474157681032583	No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATG	951	0.15077790937153673	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	947	0.1501437225813305	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	930	0.1474484287229539	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	904	0.14332621458661327	No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	872	0.13825272026496324	No Hit
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCC	758	0.12017839674408502	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	745	0.1181172896759147	No Hit
GAACAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAA	677	0.1073361142424084	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.002695293858376577	0.0
3	0.0	0.0	0.0	0.00428076083389221	0.0
4	0.0	0.0	0.0	0.00570768111185628	0.0
5	0.0	0.0	0.0	0.0065004145996140975	0.0
6	0.0	0.0	0.0	0.006658961297165661	0.0
7	0.0	0.0	0.0	0.007293148087371914	0.0
8	0.0	0.0	0.0	0.007768788180026604	0.0
9	0.0	0.0	0.0	0.014903389569846956	0.0
10	0.0	0.0	0.0	0.022989271144976686	0.0
11	1.5854669755156336E-4	0.0	0.0	0.028538405559281402	0.0
12	1.5854669755156336E-4	0.0	0.0	0.03377044657848299	0.0
13	1.5854669755156336E-4	0.0	0.0	0.03519736685644707	0.0
14	1.5854669755156336E-4	0.0	0.0	0.038526847505029896	0.0
15	1.5854669755156336E-4	0.0	0.0	0.0493080229385362	0.0
16	1.5854669755156336E-4	0.0	0.0	0.0748340412443379	0.0
17	1.5854669755156336E-4	0.0	0.0	0.10543355387178963	0.0
18	1.5854669755156336E-4	0.0	0.0	0.11764164958326001	0.0
19	1.5854669755156336E-4	0.0	0.0	0.14078946742578827	0.0
20	1.5854669755156336E-4	0.0	0.0	0.15553431029808365	0.0
21	3.170933951031267E-4	0.0	0.0	0.20024447900762452	0.0
22	3.170933951031267E-4	0.0	0.0	0.2823716683393343	0.0
23	3.170933951031267E-4	0.0	0.0	0.41951456172143664	0.0
24	3.170933951031267E-4	0.0	0.0	0.6127829860367924	0.0
25	3.170933951031267E-4	0.0	0.0	0.6559076877708175	0.0
26	3.170933951031267E-4	0.0	0.0	0.7087037380554881	0.0
27	3.170933951031267E-4	0.0	0.0	0.753096813369926	0.0
28	3.170933951031267E-4	0.0	0.0	0.8058928636545966	0.0
29	3.170933951031267E-4	0.0	0.0	0.9113264175263861	0.0
30	3.170933951031267E-4	0.0	0.0	1.0934965730131325	0.0
31	3.170933951031267E-4	0.0	0.0	1.6392143059856135	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTCTTT	25	0.005494486	29.6	27
GTACTAG	25	0.005494486	29.6	1
TTAGACC	40	5.9359452E-5	27.75	4
GTTAGAC	35	8.864938E-4	26.428572	3
GTCCTAA	40	0.0019302265	23.125	1
CAGTCGG	540	0.0	21.583334	10
AGTCGGT	540	0.0	21.583334	11
CGCCTTA	460	0.0	20.51087	25
GCAGTCG	570	0.0	20.447369	9
CCGTCTT	210	0.0	20.261906	37
GAAACCG	55	5.139559E-4	20.181818	5
GGAACGC	55	5.139559E-4	20.181818	8
GGCAGTC	600	0.0	20.041666	8
CTCGCCT	455	0.0	19.923077	23
GATTCCT	550	0.0	19.845453	18
CTTATTG	540	0.0	19.527779	28
TCGCCTT	475	0.0	19.473684	24
GCCGTCT	220	0.0	19.340908	36
TCGGTGA	575	0.0	19.304348	13
CGGTGAT	585	0.0	19.290598	14
>>END_MODULE