FastQCFastQC Report
Fri 10 Feb 2017
ERR1630698.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630698.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1396059
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT110750.7933045809668502No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT78970.5656637720898615No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT77100.5522689227317756No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41630.2981965661909704No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGACG500.00703561918.53
TAGGACG609.2381675E-418.54
TACAGCG609.2381675E-418.57
CGCCTTA1800.017.47222125
ATGGGCG852.7237937E-517.4117645
ACAGCGG852.7237937E-517.4117648
TAAGCGT650.001580163717.0769234
TCGCCAT2500.017.0213
TCAATAC1201.0416443E-716.9583343
ACAGCGT1107.8105586E-716.8181828
ATAACGA1107.8105586E-716.81818212
GTATAGG904.4470573E-516.4444451
CGAACGA1151.2425862E-616.08695616
CTATAGT1151.2425862E-616.0869564
ATACCGT1151.2425862E-616.0869566
ACGAACG1151.2425862E-616.08695615
ATCTCGT2200.015.97727337
TTCGGGC1302.5899317E-715.65384635
CGCTAGA1557.210474E-915.51612937
AGTCGGT2150.015.48837211
Produced by FastQC (version 0.11.5)