FastQCFastQC Report
Fri 10 Feb 2017
ERR1630697.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630697.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1593797
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT98860.6202797470443224No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT93480.5865238797663692No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT77200.4843778724643101No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48540.3045557244743214No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA20090.12605118468663198No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC19730.12379242776840464No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTTA1001.2907549E-820.3525
AGTCGGT3750.019.73333211
TATACTG1800.019.5277775
GCAGTCG3850.019.220789
GACGATA500.007036046618.57
AATACGC500.007036046618.55
TCGGTGA4300.018.06976713
TACGGGT1054.8001493E-717.6190474
GACGGAC2550.017.4117647
TAATACT3850.016.8181824
ACGGACC2750.016.8181828
GTTATAC1005.880658E-616.6500023
TCTGTCG2250.016.4444458
CGGTGAT4400.016.39772814
CAAGACG3050.016.377054
CGGTCCA1251.6587728E-716.2810
CAGTCGG4550.016.26373510
CGGACCA2850.016.228079
CGCTTAT1151.2428554E-616.08695626
CGCAAGA3000.016.0333332
Produced by FastQC (version 0.11.5)