FastQCFastQC Report
Fri 10 Feb 2017
ERR1630696.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630696.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1957296
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT109030.5570440035641007No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT103580.5291994670198069No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT85520.43692931472807384No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60320.30818026501867885No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGGC555.144869E-420.18181836
TTAACGG555.144869E-420.18181835
AAGACGG4100.018.5000025
CGAATTA1156.411756E-817.6956515
GGTATCA51400.017.6001951
ACGGACC4250.017.4117668
CGTTATA650.001580470117.0769232
TCCAACG650.001580470117.0769232
CGCAAGA4400.016.8181822
GCGCAAG4500.016.4444431
GACGGAC4500.016.4444437
CGGACCA4600.016.0869569
AGTCGGT3050.015.77049311
CAAGACG4950.015.696974
CGAGCCG4050.015.53086415
CGGTCCA2900.015.31034510
TACCGCA4400.015.13636428
TATACTG2850.014.9298265
GCTTTAT5950.014.9243691
GAGCGAA5300.014.660377516
Produced by FastQC (version 0.11.5)