##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630696.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1957296
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.100216829748796	33.0	31.0	34.0	30.0	34.0
2	32.26650542380917	34.0	31.0	34.0	30.0	34.0
3	32.371267299376285	34.0	31.0	34.0	30.0	34.0
4	35.94021292640459	37.0	35.0	37.0	35.0	37.0
5	35.90038144460521	37.0	35.0	37.0	35.0	37.0
6	35.97813003245294	37.0	35.0	37.0	35.0	37.0
7	35.92563822743213	37.0	35.0	37.0	35.0	37.0
8	35.93145237102615	37.0	35.0	37.0	35.0	37.0
9	37.634339926102136	39.0	37.0	39.0	35.0	39.0
10	37.562240458264874	39.0	37.0	39.0	35.0	39.0
11	37.65199591681585	39.0	37.0	39.0	35.0	39.0
12	37.600971442234595	39.0	37.0	39.0	35.0	39.0
13	37.63904079914331	39.0	37.0	39.0	35.0	39.0
14	38.93699726561542	40.0	38.0	41.0	36.0	41.0
15	38.94667030433823	40.0	38.0	41.0	36.0	41.0
16	38.903359021834206	40.0	38.0	41.0	35.0	41.0
17	38.909251334494115	40.0	38.0	41.0	35.0	41.0
18	38.90645461902543	40.0	38.0	41.0	35.0	41.0
19	38.964636416770894	40.0	38.0	41.0	35.0	41.0
20	38.94396708520326	40.0	38.0	41.0	35.0	41.0
21	38.89644795677302	40.0	38.0	41.0	35.0	41.0
22	38.82364905461412	40.0	38.0	41.0	35.0	41.0
23	38.765426384154466	40.0	38.0	41.0	35.0	41.0
24	38.714662984035115	40.0	38.0	41.0	35.0	41.0
25	38.66857950969092	40.0	38.0	41.0	34.0	41.0
26	38.537510933451046	40.0	38.0	41.0	34.0	41.0
27	38.40306065102059	40.0	38.0	41.0	34.0	41.0
28	38.297280533961136	40.0	38.0	41.0	34.0	41.0
29	38.22145449640729	40.0	38.0	41.0	34.0	41.0
30	38.12788305907742	40.0	37.0	41.0	33.0	41.0
31	38.06917144877423	40.0	37.0	41.0	33.0	41.0
32	37.96242264838839	40.0	37.0	41.0	33.0	41.0
33	37.85508425910031	40.0	37.0	41.0	33.0	41.0
34	37.78593273577425	40.0	37.0	41.0	33.0	41.0
35	37.69949307616221	40.0	37.0	41.0	33.0	41.0
36	37.64719132926241	40.0	37.0	41.0	33.0	41.0
37	37.49840443141967	40.0	37.0	41.0	33.0	41.0
38	37.33965736403692	40.0	36.0	41.0	32.0	41.0
39	37.260491003915604	40.0	36.0	41.0	32.0	41.0
40	37.061090913178184	40.0	36.0	41.0	31.0	41.0
41	36.9851524756603	40.0	36.0	41.0	31.0	41.0
42	36.82224916415299	39.0	35.0	41.0	31.0	41.0
43	36.05384315913383	39.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	8.0
10	7.0
11	5.0
12	5.0
13	6.0
14	4.0
15	4.0
16	9.0
17	25.0
18	53.0
19	120.0
20	258.0
21	450.0
22	921.0
23	1556.0
24	2582.0
25	4083.0
26	6433.0
27	9764.0
28	14242.0
29	19983.0
30	27113.0
31	35267.0
32	44996.0
33	58998.0
34	78326.0
35	105131.0
36	147188.0
37	235600.0
38	423225.0
39	740932.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.62656644677146	19.293709280558485	12.0791643164856	24.00055995618445
2	18.93755466725523	21.242469202409854	34.25920249160066	25.56077363873425
3	20.886927679819507	23.892604899820977	29.024327439487944	26.196139980871568
4	14.05311204845869	15.205722588714227	34.48522860109048	36.2559367617366
5	14.117026755278712	37.82953625818476	33.767554830746086	14.28588215579044
6	34.847616303308236	34.24050322485715	15.8847716441458	15.027108827688812
7	28.793754240544096	30.912187017191062	21.539409471025333	18.754649271239508
8	28.342315112277344	31.898292338001	20.199857354227465	19.559535195494192
9	27.088493513500257	13.235351219233063	20.704839738087646	38.97131552917903
10	17.57797492050257	26.879378489507978	32.48077960615053	23.06186698383893
11	34.96619826536201	21.969492606126003	21.39451569920952	21.669793429302466
12	22.232457431068166	26.394219372031618	28.729124261225692	22.644198935674524
13	30.03822620594943	19.889326908142664	25.873654265885182	24.198792620022726
14	22.991463733640696	21.51243347965765	26.07234674775813	29.423756038943523
15	25.95826078426564	27.593986806287855	23.357836525492313	23.08991588395419
16	24.754661533053763	26.305372309553587	25.167629219085924	23.772336938306726
17	23.143152594191168	26.535894417604695	26.30087631099231	24.020076677211826
18	22.944613384996444	24.647932658116094	28.44015417187794	23.967299785009523
19	23.538289558656434	25.42671113617971	28.189042434051874	22.845956871111984
20	24.478055439749532	24.460837808895537	27.812093827402702	23.24901292395223
21	25.09783905960059	24.91738602643647	27.16788876082105	22.816886153141887
22	24.649720839362057	24.95958710384122	27.384105418904447	23.006586637892276
23	23.685431329752884	24.98201600575488	27.468405391928457	23.86414727256378
24	23.26137691999575	25.868749540181966	27.58857117165722	23.28130236816506
25	23.652426613041666	25.5851439945721	27.246262190287005	23.516167202099222
26	23.414496325542995	26.184644530004658	27.38032469284155	23.020534451610793
27	23.818574196238078	25.195678119201183	27.26210036703697	23.723647317523767
28	22.99345627845763	25.613703803614783	27.943959421569346	23.44888049635824
29	22.613953127171364	26.157515265958754	28.1610957157221	23.067435891147788
30	22.860619957328886	26.51959642282005	27.774031112310045	22.845752507541015
31	23.006893183248728	26.818375963574237	27.06085334052693	23.113877512650106
32	22.388335744823472	26.129057638701557	27.31528598638121	24.167320630093762
33	22.100745109579748	26.059778388143645	28.085072467322263	23.754404034954348
34	23.47319976130335	25.427886226712772	27.685030777153784	23.413883234830095
35	22.916360121310216	25.567415454790694	28.108778641554473	23.40744578234462
36	22.108051107241828	26.588620218914254	27.68002386966509	23.623304804178826
37	22.966275923518978	25.517142016332738	27.964855596700755	23.55172646344753
38	22.572773867621454	25.296276087009833	28.250096050878355	23.88085399449036
39	22.613135672887495	25.151688860550475	28.411338908371548	23.823836558190482
40	21.878193180796366	25.20369938936165	29.10065723324423	23.817450196597754
41	21.148308687086676	25.387933148588665	29.592049439635087	23.871708724689572
42	21.729518682917657	25.427170954214382	29.521390734973146	23.32191962789481
43	21.318185905453237	24.776937162289197	29.431215309283825	24.473661622973736
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	596.0
1	537.0
2	478.0
3	1332.0
4	2186.0
5	2186.0
6	2898.5
7	3611.0
8	3559.0
9	3507.0
10	5076.0
11	6645.0
12	6645.0
13	11200.5
14	15756.0
15	20756.0
16	25756.0
17	24472.0
18	23188.0
19	23188.0
20	26543.0
21	29898.0
22	27345.5
23	24793.0
24	27662.0
25	30531.0
26	30531.0
27	34530.0
28	38529.0
29	43494.0
30	48459.0
31	54503.5
32	60548.0
33	60548.0
34	67044.5
35	73541.0
36	80077.0
37	86613.0
38	93160.0
39	99707.0
40	99707.0
41	103718.0
42	107729.0
43	109492.5
44	111256.0
45	113284.5
46	115313.0
47	115313.0
48	120135.0
49	124957.0
50	124787.5
51	124618.0
52	127552.0
53	130486.0
54	130486.0
55	128024.5
56	125563.0
57	121720.0
58	117877.0
59	113326.5
60	108776.0
61	108776.0
62	100989.5
63	93203.0
64	83307.5
65	73412.0
66	63537.5
67	53663.0
68	53663.0
69	45771.0
70	37879.0
71	32345.5
72	26812.0
73	20102.0
74	13392.0
75	13392.0
76	9988.0
77	6584.0
78	5320.5
79	4057.0
80	3391.0
81	2725.0
82	2725.0
83	2125.0
84	1525.0
85	1445.0
86	1365.0
87	1258.5
88	1152.0
89	1152.0
90	842.0
91	532.0
92	290.0
93	48.0
94	37.0
95	26.0
96	26.0
97	14.5
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1957296.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.62543635337632
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.47415987104937	51.282107920852695
2	8.065373676955993	9.456721023291607
3	1.8254093820902797	3.210462646457691
4	0.7017636427771116	1.645647990989723
5	0.3821466486610981	1.1201757014368634
6	0.25134009186064593	0.8840953535056835
7	0.1710972566023493	0.7021455929024829
8	0.12125720486169198	0.568700523680595
9	0.09349340562135551	0.49329825306436564
>10	0.704944216222139	8.372392158210939
>50	0.10080881091481866	4.153248179184234
>100	0.09808421694416412	11.598486196492233
>500	0.007678436539811845	3.0594457484310222
>1k	0.0020941190563123213	1.6194718679392355
>5k	1.745099213593601E-4	0.7460241233780539
>10k+	1.745099213593601E-4	1.0875767201824469
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	10903	0.5570440035641007	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	10358	0.5291994670198069	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8552	0.43692931472807384	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6032	0.30818026501867885	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	5.109089274182341E-5	0.0
2	0.0	0.0	0.0	6.641816056437044E-4	0.0
3	0.0	0.0	0.0	0.0010218178548364683	0.0
4	0.0	0.0	0.0	0.0021458174951565832	0.0
5	0.0	0.0	0.0	0.0022479992806402303	0.0
6	0.0	0.0	0.0	0.0022990901733820537	0.0
7	0.0	0.0	0.0	0.0028610899935421112	0.0
8	0.0	0.0	0.0	0.0031165444572512284	0.0
9	0.0	0.0	0.0	0.0058754526653096925	0.0
10	5.109089274182341E-5	0.0	0.0	0.007765815696757159	0.0
11	5.109089274182341E-5	0.0	0.0	0.009247451586270038	0.0
12	5.109089274182341E-5	5.109089274182341E-5	0.0	0.01093345104675021	0.0
13	5.109089274182341E-5	5.109089274182341E-5	0.0	0.011342178188684798	0.0
14	5.109089274182341E-5	5.109089274182341E-5	0.0	0.012517268721746737	0.0
15	5.109089274182341E-5	5.109089274182341E-5	0.0	0.01466308621690332	0.0
16	5.109089274182341E-5	5.109089274182341E-5	0.0	0.01946563013463472	0.0
17	5.109089274182341E-5	5.109089274182341E-5	0.0	0.02411490137414065	0.0
18	5.109089274182341E-5	5.109089274182341E-5	0.0	0.026465082440264527	0.0
19	5.109089274182341E-5	1.0218178548364682E-4	0.0	0.030296899395901283	0.0
20	5.109089274182341E-5	1.0218178548364682E-4	0.0	0.03305580760395975	0.0
21	5.109089274182341E-5	1.0218178548364682E-4	0.0	0.04010635080233138	0.0
22	5.109089274182341E-5	1.0218178548364682E-4	0.0	0.05287907398778723	0.0
23	5.109089274182341E-5	1.0218178548364682E-4	0.0	0.07193597698048737	0.0
24	5.109089274182341E-5	1.5327267822547023E-4	0.0	0.10121105852155218	0.0
25	5.109089274182341E-5	1.5327267822547023E-4	0.0	0.10882360154008387	0.0
26	5.109089274182341E-5	1.5327267822547023E-4	0.0	0.12103432490537967	0.0
27	5.109089274182341E-5	1.5327267822547023E-4	0.0	0.13881395557953422	0.0
28	5.109089274182341E-5	1.5327267822547023E-4	0.0	0.178511579239931	0.0
29	5.109089274182341E-5	2.0436357096729365E-4	0.0	0.23706174232206065	0.0
30	5.109089274182341E-5	2.0436357096729365E-4	0.0	0.3262153501565425	0.0
31	5.109089274182341E-5	2.0436357096729365E-4	0.0	0.6417016128373021	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGGC	55	5.144869E-4	20.181818	36
TTAACGG	55	5.144869E-4	20.181818	35
AAGACGG	410	0.0	18.500002	5
CGAATTA	115	6.411756E-8	17.69565	15
GGTATCA	5140	0.0	17.600195	1
ACGGACC	425	0.0	17.411766	8
CGTTATA	65	0.0015804701	17.076923	2
TCCAACG	65	0.0015804701	17.076923	2
CGCAAGA	440	0.0	16.818182	2
GCGCAAG	450	0.0	16.444443	1
GACGGAC	450	0.0	16.444443	7
CGGACCA	460	0.0	16.086956	9
AGTCGGT	305	0.0	15.770493	11
CAAGACG	495	0.0	15.69697	4
CGAGCCG	405	0.0	15.530864	15
CGGTCCA	290	0.0	15.310345	10
TACCGCA	440	0.0	15.136364	28
TATACTG	285	0.0	14.929826	5
GCTTTAT	595	0.0	14.924369	1
GAGCGAA	530	0.0	14.6603775	16
>>END_MODULE