FastQCFastQC Report
Fri 10 Feb 2017
ERR1630695.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630695.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences810940
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT141461.7443953930007152No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT133771.6495671689644116No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT111521.3751942190544308No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88431.0904629195748143No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26230.3234517966803956No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT21330.2630280908575234No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19820.2444077243692505No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19590.24157150960613608No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT17190.21197622512146397No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14390.17744839322267986No Hit
GTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGC13930.17177596369645104No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA12930.159444595161171No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG12600.15537524354452859No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT12140.14970281401829974No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT11900.14674328556983254No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC11290.1392211507633117No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG10750.13256221175426047No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC10370.12787629171085407No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG10180.12553333168915087No Hit
AATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTT10070.12417688115027006No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG9920.12232717586997804No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG9830.12121735270180284No Hit
GTCTGTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCC9710.11973758847756924No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC9700.11961427479221644No Hit
GTGCCAGGTGGGGAGTTTGACTGGGGCGGTACACCTGTCAAAC9530.11751794214121883No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCT9470.11677806002910202No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC9430.11628480528769083No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9340.11517498211951563No Hit
ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGC9110.1123387673564012No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA8960.11048906207610922No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC8930.11011912102005081No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCG8830.10888598416652279No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAA8730.10765284731299479No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA8590.10592645571805559No Hit
CCTTAGGACACCTGCGTTACCGTTTGACAGGTGTACCGCCCCA8590.10592645571805559No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA8320.10259698621352999No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC8300.10235035884282437No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8250.10173379041606037No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATGA358.8671345E-426.428573
AAGACGG3600.023.1250025
CCGCTTA802.723391E-823.12525
ACTGTAG400.001930699723.1258
AGCGTCA903.8235157E-922.6111133
ATATACG753.7379505E-722.26
ACGCTAT753.7379505E-722.210
CGCTTAT753.7379505E-722.226
TCTAATA1201.0913936E-1121.5833322
TTGGCGC957.1377144E-921.42105310
GCGCTTG1059.822543E-1021.1428576
TACTGGT1151.3460522E-1020.9130447
TACGCTA806.954924E-720.81259
TTGCTAT450.003824850320.5555571
CGTCTCT450.003824850320.55555720
CAGCGTC1001.2883902E-820.352
GACGGAC4000.020.357
GCAGCGT1001.2883902E-820.351
ACGGACC4100.020.3048768
CAAGACG4150.020.0602424
Produced by FastQC (version 0.11.5)